Experimental strategies to achieve efficient targeted knock-in via tandem paired nicking

Tandem paired nicking (TPN) is a method of genome editing that enables precise and relatively efficient targeted knock-in without appreciable restraint by p53-mediated DNA damage response. TPN is initiated by introducing two site-specific nicks on the same DNA strand using Cas9 nickases in such a wa...

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Detalles Bibliográficos
Autores principales: Rahman, Md. Lutfur, Hyodo, Toshinori, Karnan, Sivasundaram, Ota, Akinobu, Hasan, Muhammad Nazmul, Mihara, Yuko, Wahiduzzaman, Md, Tsuzuki, Shinobu, Hosokawa, Yoshitaka, Konishi, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604973/
https://www.ncbi.nlm.nih.gov/pubmed/34799652
http://dx.doi.org/10.1038/s41598-021-01978-w

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