Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays

Specific antigen recognition by T cell receptor (TCR) activates TCR signaling pathway, leading to T cell proliferation and differentiation into effector and memory cells. Herein, we describe protocols for TCR stimulation assays, including procedures for the isolation and enrichment of mouse splenic...

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Autores principales: Grosjean, Clémence, Quessada, Julie, Nozais, Mathis, Loosveld, Marie, Payet-Bornet, Dominique, Mionnet, Cyrille
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605083/
https://www.ncbi.nlm.nih.gov/pubmed/34825221
http://dx.doi.org/10.1016/j.xpro.2021.100961
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author Grosjean, Clémence
Quessada, Julie
Nozais, Mathis
Loosveld, Marie
Payet-Bornet, Dominique
Mionnet, Cyrille
author_facet Grosjean, Clémence
Quessada, Julie
Nozais, Mathis
Loosveld, Marie
Payet-Bornet, Dominique
Mionnet, Cyrille
author_sort Grosjean, Clémence
collection PubMed
description Specific antigen recognition by T cell receptor (TCR) activates TCR signaling pathway, leading to T cell proliferation and differentiation into effector and memory cells. Herein, we describe protocols for TCR stimulation assays, including procedures for the isolation and enrichment of mouse splenic T cells for ex vivo TCR stimulation with anti-CD3/CD28 antibodies, and the use of ovalbumin-OT-II mouse model for in vivo TCR stimulation. We applied this protocol to show that MYC protein is essential for T cell proliferation and differentiation. For complete details on the use and execution of this protocol, please refer to Nozais et al. (2021).
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spelling pubmed-86050832021-11-24 Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays Grosjean, Clémence Quessada, Julie Nozais, Mathis Loosveld, Marie Payet-Bornet, Dominique Mionnet, Cyrille STAR Protoc Protocol Specific antigen recognition by T cell receptor (TCR) activates TCR signaling pathway, leading to T cell proliferation and differentiation into effector and memory cells. Herein, we describe protocols for TCR stimulation assays, including procedures for the isolation and enrichment of mouse splenic T cells for ex vivo TCR stimulation with anti-CD3/CD28 antibodies, and the use of ovalbumin-OT-II mouse model for in vivo TCR stimulation. We applied this protocol to show that MYC protein is essential for T cell proliferation and differentiation. For complete details on the use and execution of this protocol, please refer to Nozais et al. (2021). Elsevier 2021-11-16 /pmc/articles/PMC8605083/ /pubmed/34825221 http://dx.doi.org/10.1016/j.xpro.2021.100961 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Grosjean, Clémence
Quessada, Julie
Nozais, Mathis
Loosveld, Marie
Payet-Bornet, Dominique
Mionnet, Cyrille
Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays
title Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays
title_full Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays
title_fullStr Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays
title_full_unstemmed Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays
title_short Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays
title_sort isolation and enrichment of mouse splenic t cells for ex vivo and in vivo t cell receptor stimulation assays
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605083/
https://www.ncbi.nlm.nih.gov/pubmed/34825221
http://dx.doi.org/10.1016/j.xpro.2021.100961
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