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Quantifying the global binding and target-search dynamics of epigenetic regulatory factors using live-cell single-molecule tracking

This protocol provides instructions to track the global dynamics of single epigenetic regulatory factors in live cells. We describe an approach to generate cell lines that stably express HaloTag-fused proteins. We then use live-cell single-molecule tracking to obtain kinetic populations and residenc...

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Detalles Bibliográficos
Autores principales:	Brown, Kyle, Kent, Samantha, Ren, Xiaojun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605088/ https://www.ncbi.nlm.nih.gov/pubmed/34825219 http://dx.doi.org/10.1016/j.xpro.2021.100959

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