Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing

Teeth and the surrounding periodontal tissues are affected by many pathologies that compromise their integrity and significantly affect life quality. The study of the main dental tissues, the dental pulp and periodontium, is made arduous by their close association with highly mineralized tissues (de...

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Detalles Bibliográficos
Autores principales: Pagella, Pierfrancesco, Stadlinger, Bernd, Mitsiadis, Thimios A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605092/
https://www.ncbi.nlm.nih.gov/pubmed/34825216
http://dx.doi.org/10.1016/j.xpro.2021.100953
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author Pagella, Pierfrancesco
Stadlinger, Bernd
Mitsiadis, Thimios A.
author_facet Pagella, Pierfrancesco
Stadlinger, Bernd
Mitsiadis, Thimios A.
author_sort Pagella, Pierfrancesco
collection PubMed
description Teeth and the surrounding periodontal tissues are affected by many pathologies that compromise their integrity and significantly affect life quality. The study of the main dental tissues, the dental pulp and periodontium, is made arduous by their close association with highly mineralized tissues (dentin, cementum, and alveolar bone). Here we describe a protocol to isolate all cells composing human dental pulp and periodontium for single-cell RNA sequencing analysis. For complete details on the use and execution of this protocol, please refer to Pagella et al. (2021).
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spelling pubmed-86050922021-11-24 Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing Pagella, Pierfrancesco Stadlinger, Bernd Mitsiadis, Thimios A. STAR Protoc Protocol Teeth and the surrounding periodontal tissues are affected by many pathologies that compromise their integrity and significantly affect life quality. The study of the main dental tissues, the dental pulp and periodontium, is made arduous by their close association with highly mineralized tissues (dentin, cementum, and alveolar bone). Here we describe a protocol to isolate all cells composing human dental pulp and periodontium for single-cell RNA sequencing analysis. For complete details on the use and execution of this protocol, please refer to Pagella et al. (2021). Elsevier 2021-11-17 /pmc/articles/PMC8605092/ /pubmed/34825216 http://dx.doi.org/10.1016/j.xpro.2021.100953 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Pagella, Pierfrancesco
Stadlinger, Bernd
Mitsiadis, Thimios A.
Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing
title Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing
title_full Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing
title_fullStr Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing
title_full_unstemmed Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing
title_short Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing
title_sort isolation of dental pulp and periodontal cells from human teeth for single-cell rna sequencing
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605092/
https://www.ncbi.nlm.nih.gov/pubmed/34825216
http://dx.doi.org/10.1016/j.xpro.2021.100953
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AT mitsiadisthimiosa isolationofdentalpulpandperiodontalcellsfromhumanteethforsinglecellrnasequencing

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