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Optimized electroporation of CRISPR-Cas9/gRNA ribonucleoprotein complex for selection-free homologous recombination in human pluripotent stem cells

Selection-free, scarless genome editing in human pluripotent stem cells (PSCs) by utilizing ribonucleoprotein (RNP) of CRISPR-Cas9 is a useful tool for a variety of applications. However, the process can be hampered by time-consuming subcloning steps and inefficient delivery of the RNP complex and s...

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Detalles Bibliográficos
Autores principales:	Xu, Huaigeng, Kita, Yuto, Bang, Uikyu, Gee, Peter, Hotta, Akitsu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605105/ https://www.ncbi.nlm.nih.gov/pubmed/34825222 http://dx.doi.org/10.1016/j.xpro.2021.100965

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