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Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil

Phenolic compounds are regarded as the most abundant plant metabolites that are known to decompose progressively into soils, likened to other soil organic materials. Once assimilated into soils, they can control soil processes, including organic matter decomposition and nutrient cycling. Established...

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Autores principales: Akomeng, Nicholas, Adusei, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605349/
https://www.ncbi.nlm.nih.gov/pubmed/34841107
http://dx.doi.org/10.1016/j.heliyon.2021.e08388
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author Akomeng, Nicholas
Adusei, Stephen
author_facet Akomeng, Nicholas
Adusei, Stephen
author_sort Akomeng, Nicholas
collection PubMed
description Phenolic compounds are regarded as the most abundant plant metabolites that are known to decompose progressively into soils, likened to other soil organic materials. Once assimilated into soils, they can control soil processes, including organic matter decomposition and nutrient cycling. Established that phenolic compounds can influence nutrients availability and soil quality, it becomes crucial to investigate into soil phenolics through the application of appropriate extraction technique and quantification of ​total phenolic content ​in soils. This study therefore aimed at utilizing ethanol, hexane and diethyl ether organic solvents to extract and quantify total phenolic content of soil, sampled from a vegetable growing area. Conventional organic solvent extraction method was employed to extract phenolics, while spectrophotometric technique was utilized to quantify total phenolic content. The highest extraction yield of 34.52% was achieved with ethanol followed by diethyl ether (28.23%) and hexane (25.47%). Interestingly, hexane, which had the least extraction yield, rather recorded the highest phenolics concentration of 5.50 ± 0.02 mgGAE/g, with ethanol producing a concentration of 2.04 ± 0.05 mgGAE/g and 3.82 ± 0.01 mgGAE/g for diethyl ether. The percent recovery, limit of detection (LOD) and limit of quantification (LOQ) of phenolic compounds were found to be 102%, 0.8 mg/g and 1.5 mg/g for ethanol; 96%, 0.6 mg/g and 1.2 mg/g for diethyl ether and 94%, 0.4 mg/g and 1.0 mg/g for hexane respectively. These results indicate that for an extraction efficiency and greater yield, the use of ethanol as solvent is preferred whereas extraction using hexane is suitable for total phenolics quantification. The findings of this study have provided a vital insight regarding the influence of organic solvents on the extractability and quantification of total phenolic content of soil.
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spelling pubmed-86053492021-11-26 Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil Akomeng, Nicholas Adusei, Stephen Heliyon Research Article Phenolic compounds are regarded as the most abundant plant metabolites that are known to decompose progressively into soils, likened to other soil organic materials. Once assimilated into soils, they can control soil processes, including organic matter decomposition and nutrient cycling. Established that phenolic compounds can influence nutrients availability and soil quality, it becomes crucial to investigate into soil phenolics through the application of appropriate extraction technique and quantification of ​total phenolic content ​in soils. This study therefore aimed at utilizing ethanol, hexane and diethyl ether organic solvents to extract and quantify total phenolic content of soil, sampled from a vegetable growing area. Conventional organic solvent extraction method was employed to extract phenolics, while spectrophotometric technique was utilized to quantify total phenolic content. The highest extraction yield of 34.52% was achieved with ethanol followed by diethyl ether (28.23%) and hexane (25.47%). Interestingly, hexane, which had the least extraction yield, rather recorded the highest phenolics concentration of 5.50 ± 0.02 mgGAE/g, with ethanol producing a concentration of 2.04 ± 0.05 mgGAE/g and 3.82 ± 0.01 mgGAE/g for diethyl ether. The percent recovery, limit of detection (LOD) and limit of quantification (LOQ) of phenolic compounds were found to be 102%, 0.8 mg/g and 1.5 mg/g for ethanol; 96%, 0.6 mg/g and 1.2 mg/g for diethyl ether and 94%, 0.4 mg/g and 1.0 mg/g for hexane respectively. These results indicate that for an extraction efficiency and greater yield, the use of ethanol as solvent is preferred whereas extraction using hexane is suitable for total phenolics quantification. The findings of this study have provided a vital insight regarding the influence of organic solvents on the extractability and quantification of total phenolic content of soil. Elsevier 2021-11-14 /pmc/articles/PMC8605349/ /pubmed/34841107 http://dx.doi.org/10.1016/j.heliyon.2021.e08388 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Akomeng, Nicholas
Adusei, Stephen
Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil
title Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil
title_full Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil
title_fullStr Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil
title_full_unstemmed Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil
title_short Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil
title_sort organic solvent extraction and spectrophotometric quantification of total phenolic content of soil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605349/
https://www.ncbi.nlm.nih.gov/pubmed/34841107
http://dx.doi.org/10.1016/j.heliyon.2021.e08388
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