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Resistance training mitigates hepato-cardiac changes and muscle mitochondrial protein reductions in rats with diet-induced obesity

AIM: To investigate the effect of resistance training (RT) on hepatocardiovascular and muscle mitochondrial parameters in rats that were fed a high-calorie diet for 12 weeks. MAIN METHODS: The animals were divided into four groups: control (C), exercise (E), obese (O), and obese plus exercise (OE)....

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Autores principales: Laurindo, Caroline Pancera, Rego Gregorio, Karen C., Rippi Moreno, Ana Caroline, Viudes Agostinho, Julia Maia, Campos, Evelyn Carvalho, Nai, Gisele Alborghetti, Nunes, Maria Tereza, Seraphim, Patrícia Monteiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605435/
https://www.ncbi.nlm.nih.gov/pubmed/34841103
http://dx.doi.org/10.1016/j.heliyon.2021.e08374
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author Laurindo, Caroline Pancera
Rego Gregorio, Karen C.
Rippi Moreno, Ana Caroline
Viudes Agostinho, Julia Maia
Campos, Evelyn Carvalho
Nai, Gisele Alborghetti
Nunes, Maria Tereza
Seraphim, Patrícia Monteiro
author_facet Laurindo, Caroline Pancera
Rego Gregorio, Karen C.
Rippi Moreno, Ana Caroline
Viudes Agostinho, Julia Maia
Campos, Evelyn Carvalho
Nai, Gisele Alborghetti
Nunes, Maria Tereza
Seraphim, Patrícia Monteiro
author_sort Laurindo, Caroline Pancera
collection PubMed
description AIM: To investigate the effect of resistance training (RT) on hepatocardiovascular and muscle mitochondrial parameters in rats that were fed a high-calorie diet for 12 weeks. MAIN METHODS: The animals were divided into four groups: control (C), exercise (E), obese (O), and obese plus exercise (OE). Group E and OE rats performed resistance training by climbing on a vertical ladder with load attached to the end of the tail (1×/day, 3×/week, for 12 weeks). Group O and OE rats were fed a high-calorie diet containing chow and a cafeteria diet for 12 weeks. Under anesthesia, the heart and liver were removed for histopathological analysis, and the gastrocnemius muscle was removed for Western blotting. KEY FINDINGS: Group O rats were heavier, with increased fat mass, elevated fasting glycemia, and total triglycerides, and exhibited a significant number of Kupffer cells and diffuse steatosis in the liver. Group O rats also showed increased thickness of the right ventricle, septum, and pulmonary artery. All of these parameters were attenuated by RT. PGC1-α protein levels were increased in both exercise groups. The protein levels of OXPHOS complexes III, IV, and V were reduced in Group O, while RT prevented this alteration. SIGNIFICANCE: RT exerts a protective effect against hepato-cardiac alterations and prevents changes in the muscle mitochondrial protein profile induced by a high-calorie diet.
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spelling pubmed-86054352021-11-26 Resistance training mitigates hepato-cardiac changes and muscle mitochondrial protein reductions in rats with diet-induced obesity Laurindo, Caroline Pancera Rego Gregorio, Karen C. Rippi Moreno, Ana Caroline Viudes Agostinho, Julia Maia Campos, Evelyn Carvalho Nai, Gisele Alborghetti Nunes, Maria Tereza Seraphim, Patrícia Monteiro Heliyon Research Article AIM: To investigate the effect of resistance training (RT) on hepatocardiovascular and muscle mitochondrial parameters in rats that were fed a high-calorie diet for 12 weeks. MAIN METHODS: The animals were divided into four groups: control (C), exercise (E), obese (O), and obese plus exercise (OE). Group E and OE rats performed resistance training by climbing on a vertical ladder with load attached to the end of the tail (1×/day, 3×/week, for 12 weeks). Group O and OE rats were fed a high-calorie diet containing chow and a cafeteria diet for 12 weeks. Under anesthesia, the heart and liver were removed for histopathological analysis, and the gastrocnemius muscle was removed for Western blotting. KEY FINDINGS: Group O rats were heavier, with increased fat mass, elevated fasting glycemia, and total triglycerides, and exhibited a significant number of Kupffer cells and diffuse steatosis in the liver. Group O rats also showed increased thickness of the right ventricle, septum, and pulmonary artery. All of these parameters were attenuated by RT. PGC1-α protein levels were increased in both exercise groups. The protein levels of OXPHOS complexes III, IV, and V were reduced in Group O, while RT prevented this alteration. SIGNIFICANCE: RT exerts a protective effect against hepato-cardiac alterations and prevents changes in the muscle mitochondrial protein profile induced by a high-calorie diet. Elsevier 2021-11-14 /pmc/articles/PMC8605435/ /pubmed/34841103 http://dx.doi.org/10.1016/j.heliyon.2021.e08374 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Laurindo, Caroline Pancera
Rego Gregorio, Karen C.
Rippi Moreno, Ana Caroline
Viudes Agostinho, Julia Maia
Campos, Evelyn Carvalho
Nai, Gisele Alborghetti
Nunes, Maria Tereza
Seraphim, Patrícia Monteiro
Resistance training mitigates hepato-cardiac changes and muscle mitochondrial protein reductions in rats with diet-induced obesity
title Resistance training mitigates hepato-cardiac changes and muscle mitochondrial protein reductions in rats with diet-induced obesity
title_full Resistance training mitigates hepato-cardiac changes and muscle mitochondrial protein reductions in rats with diet-induced obesity
title_fullStr Resistance training mitigates hepato-cardiac changes and muscle mitochondrial protein reductions in rats with diet-induced obesity
title_full_unstemmed Resistance training mitigates hepato-cardiac changes and muscle mitochondrial protein reductions in rats with diet-induced obesity
title_short Resistance training mitigates hepato-cardiac changes and muscle mitochondrial protein reductions in rats with diet-induced obesity
title_sort resistance training mitigates hepato-cardiac changes and muscle mitochondrial protein reductions in rats with diet-induced obesity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605435/
https://www.ncbi.nlm.nih.gov/pubmed/34841103
http://dx.doi.org/10.1016/j.heliyon.2021.e08374
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