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circ_0082375 promotes the progression of glioma by regulating Wnt7B
Circular RNAs contribute to the progression of glioma. However, the biological role and underlying mechanism of circ_0082375 in glioma remain unclear. Quantitative real-time PCR and Western blot assay were used to evaluate the expression levels of circ_0082375, microRNA-485-5p, and Wnt family member...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
De Gruyter
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605768/ https://www.ncbi.nlm.nih.gov/pubmed/34868669 http://dx.doi.org/10.1515/tnsci-2020-0181 |
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author | Meng, Xianbing Tian, Hailong Guo, Wenqiang Wang, Zhigang |
author_facet | Meng, Xianbing Tian, Hailong Guo, Wenqiang Wang, Zhigang |
author_sort | Meng, Xianbing |
collection | PubMed |
description | Circular RNAs contribute to the progression of glioma. However, the biological role and underlying mechanism of circ_0082375 in glioma remain unclear. Quantitative real-time PCR and Western blot assay were used to evaluate the expression levels of circ_0082375, microRNA-485-5p, and Wnt family member 7B (Wnt7B). The overall survival of glioma patients was estimated by the Kaplan–Meier curve. Cell proliferation, apoptosis, invasion, and migration were detected by cell counting kit-8, 5-ethynyl-2 -deoxyuridine (EdU) staining, flow cytometry, and transwell assays, respectively. Glucose level and lactate production were determined using glucose and lactate assay kits. In vitro angiogenesis assay was used to evaluate the angiogenesis of glioma cells. The interaction between microRNA (miR)-485-5p and circ_0082375 or Wnt family member 7B (Wnt7B) was verified by dual-luciferase reporter and RNA immunoprecipitation assays. A xenograft model was used to verify the function of circ_0082375 in vivo. circ_0082375 was upregulated in glioma tissues, and it was closely related to the prognosis of glioma patients. circ_0082375 knockdown suppressed cell proliferation, migration, invasion, angiogenesis, glycolysis, and epithelial-mesenchymal transition (EMT), and promoted cell apoptosis in glioma cells. irc_0082375 was a sponge of miR-485-5p, which directly targeted Wnt7B. Knockdown of circ_0082375 inhibited the malignancy, angiogenesis, and glycolysis of glioma cells in vitro by sponging miR-485-5p. Besides, circ_0082375 knockdown hampered the growth of glioma growth by regulating the miR-485-5p/Wnt7B axis in vivo. Altogether, circ_0082375 regulated miR-485-5p/Wnt7B axis to promote the malignancy, angiogenesis, and glycolysis of glioma cells, thereby contributing to the progression of glioma. |
format | Online Article Text |
id | pubmed-8605768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | De Gruyter |
record_format | MEDLINE/PubMed |
spelling | pubmed-86057682021-12-02 circ_0082375 promotes the progression of glioma by regulating Wnt7B Meng, Xianbing Tian, Hailong Guo, Wenqiang Wang, Zhigang Transl Neurosci Research Article Circular RNAs contribute to the progression of glioma. However, the biological role and underlying mechanism of circ_0082375 in glioma remain unclear. Quantitative real-time PCR and Western blot assay were used to evaluate the expression levels of circ_0082375, microRNA-485-5p, and Wnt family member 7B (Wnt7B). The overall survival of glioma patients was estimated by the Kaplan–Meier curve. Cell proliferation, apoptosis, invasion, and migration were detected by cell counting kit-8, 5-ethynyl-2 -deoxyuridine (EdU) staining, flow cytometry, and transwell assays, respectively. Glucose level and lactate production were determined using glucose and lactate assay kits. In vitro angiogenesis assay was used to evaluate the angiogenesis of glioma cells. The interaction between microRNA (miR)-485-5p and circ_0082375 or Wnt family member 7B (Wnt7B) was verified by dual-luciferase reporter and RNA immunoprecipitation assays. A xenograft model was used to verify the function of circ_0082375 in vivo. circ_0082375 was upregulated in glioma tissues, and it was closely related to the prognosis of glioma patients. circ_0082375 knockdown suppressed cell proliferation, migration, invasion, angiogenesis, glycolysis, and epithelial-mesenchymal transition (EMT), and promoted cell apoptosis in glioma cells. irc_0082375 was a sponge of miR-485-5p, which directly targeted Wnt7B. Knockdown of circ_0082375 inhibited the malignancy, angiogenesis, and glycolysis of glioma cells in vitro by sponging miR-485-5p. Besides, circ_0082375 knockdown hampered the growth of glioma growth by regulating the miR-485-5p/Wnt7B axis in vivo. Altogether, circ_0082375 regulated miR-485-5p/Wnt7B axis to promote the malignancy, angiogenesis, and glycolysis of glioma cells, thereby contributing to the progression of glioma. De Gruyter 2021-11-19 /pmc/articles/PMC8605768/ /pubmed/34868669 http://dx.doi.org/10.1515/tnsci-2020-0181 Text en © 2021 Xianbing Meng et al., published by De Gruyter https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. |
spellingShingle | Research Article Meng, Xianbing Tian, Hailong Guo, Wenqiang Wang, Zhigang circ_0082375 promotes the progression of glioma by regulating Wnt7B |
title | circ_0082375 promotes the progression of glioma by regulating Wnt7B |
title_full | circ_0082375 promotes the progression of glioma by regulating Wnt7B |
title_fullStr | circ_0082375 promotes the progression of glioma by regulating Wnt7B |
title_full_unstemmed | circ_0082375 promotes the progression of glioma by regulating Wnt7B |
title_short | circ_0082375 promotes the progression of glioma by regulating Wnt7B |
title_sort | circ_0082375 promotes the progression of glioma by regulating wnt7b |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605768/ https://www.ncbi.nlm.nih.gov/pubmed/34868669 http://dx.doi.org/10.1515/tnsci-2020-0181 |
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