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Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4
Fowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), distributed widely in the poultry farms in China. Hexon is one of the major capsid proteins associated with viral species or serotypes. However, the epitopes of Hexon protein remain largely unknown...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605946/ https://www.ncbi.nlm.nih.gov/pubmed/34800173 http://dx.doi.org/10.1186/s13568-021-01309-2 |
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author | Liu, Jingyi Mei, Nan Wang, Yaling Shi, Xinjin Chen, Hongjun |
author_facet | Liu, Jingyi Mei, Nan Wang, Yaling Shi, Xinjin Chen, Hongjun |
author_sort | Liu, Jingyi |
collection | PubMed |
description | Fowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), distributed widely in the poultry farms in China. Hexon is one of the major capsid proteins associated with viral species or serotypes. However, the epitopes of Hexon protein remain largely unknown. In this study, a monoclonal antibody (mAb) specific to Hexon protein of FAdV-4, designated as 3G8, was generated. Subsequently, the linear peptide recognized by 3G8 was mapped and identified as (213)AYGAYVK(219) using a series of overlapping peptides generated from Hexon protein. Amino acid sequence analysis revealed that the epitope recognized by 3G8 was highly conserved across all the FAdVs. The epitope was immunogenic and could be recognized by FAdV-4 positive chicken serum samples. These findings will enrich our knowledge regarding the epitope on Hexon and provide valuable information for further characterization of the antigenicity of Hexon protein. |
format | Online Article Text |
id | pubmed-8605946 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-86059462021-11-24 Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4 Liu, Jingyi Mei, Nan Wang, Yaling Shi, Xinjin Chen, Hongjun AMB Express Original Article Fowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), distributed widely in the poultry farms in China. Hexon is one of the major capsid proteins associated with viral species or serotypes. However, the epitopes of Hexon protein remain largely unknown. In this study, a monoclonal antibody (mAb) specific to Hexon protein of FAdV-4, designated as 3G8, was generated. Subsequently, the linear peptide recognized by 3G8 was mapped and identified as (213)AYGAYVK(219) using a series of overlapping peptides generated from Hexon protein. Amino acid sequence analysis revealed that the epitope recognized by 3G8 was highly conserved across all the FAdVs. The epitope was immunogenic and could be recognized by FAdV-4 positive chicken serum samples. These findings will enrich our knowledge regarding the epitope on Hexon and provide valuable information for further characterization of the antigenicity of Hexon protein. Springer Berlin Heidelberg 2021-11-20 /pmc/articles/PMC8605946/ /pubmed/34800173 http://dx.doi.org/10.1186/s13568-021-01309-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Liu, Jingyi Mei, Nan Wang, Yaling Shi, Xinjin Chen, Hongjun Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4 |
title | Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4 |
title_full | Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4 |
title_fullStr | Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4 |
title_full_unstemmed | Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4 |
title_short | Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4 |
title_sort | identification of a novel immunological epitope on hexon of fowl adenovirus serotype 4 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605946/ https://www.ncbi.nlm.nih.gov/pubmed/34800173 http://dx.doi.org/10.1186/s13568-021-01309-2 |
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