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Highly improved cloning efficiency for plasmid-based CRISPR knock-in in C. elegans

Plasmid-based CRISPR knock-in is a streamlined, scalable, and versatile approach for generating fluorescent protein tags in C. elegans (Dickinson et al. 2015; Schwartz and Jorgensen 2016). However, compared to more recent protocols that utilize commercially available Cas9/RNP products and linear DNA...

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Detalles Bibliográficos
Autores principales:	DeMott, Ella, Dickinson, Daniel J, Doonan, Ryan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Caltech Library 2021
Materias:	New Methods
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606032/ https://www.ncbi.nlm.nih.gov/pubmed/34816097 http://dx.doi.org/10.17912/micropub.biology.000499

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