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Pitfalls of Restriction Enzyme Mapping Following Generation of CRISPR Constructs

BACKGROUND: The PX330 and the related PX459 plasmids are widely used for Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas9-mediated genome editing. Screening for plasmids containing the correct sgRNA template insertion is one of the most important steps in this system. Different...

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Detalles Bibliográficos
Autores principales:	Hassani, Mehdi, Hesami, Sara, Maroofi, Nahal, Banan, Mehdi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Avicenna Research Institute 2021
Materias:	Short Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606105/ https://www.ncbi.nlm.nih.gov/pubmed/34900150

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