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Temporal phase evolution OCT for measurement of tissue deformation in the human retina in-vivo
We demonstrate the use of temporal phase evolution (TPE-) OCT methods to evaluate retinal tissue deformation in-vivo over time periods of several seconds. A custom built spectral domain (SD)-OCT system with an integrated retinal tracker, ensuring stable imaging with sub-speckle precision, was used f...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606136/ https://www.ncbi.nlm.nih.gov/pubmed/34858702 http://dx.doi.org/10.1364/BOE.440893 |
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author | Desissaire, Sylvia Schwarzhans, Florian Steiner, Stefan Vass, Clemens Fischer, Georg Pircher, Michael Hitzenberger, Christoph K. |
author_facet | Desissaire, Sylvia Schwarzhans, Florian Steiner, Stefan Vass, Clemens Fischer, Georg Pircher, Michael Hitzenberger, Christoph K. |
author_sort | Desissaire, Sylvia |
collection | PubMed |
description | We demonstrate the use of temporal phase evolution (TPE-) OCT methods to evaluate retinal tissue deformation in-vivo over time periods of several seconds. A custom built spectral domain (SD)-OCT system with an integrated retinal tracker, ensuring stable imaging with sub-speckle precision, was used for imaging. TPE-OCT measures and images phase differences between an initial reference B-scan and each of the subsequent B-scans of the evaluated temporal sequence. In order to demonstrate the precision and repeatability of the measurements, retinal nerve fiber (RNF) tissue deformations induced by retinal vessels pulsating with the heartbeat were analyzed in several healthy subjects. We show TPE maps (M-scans of phase evolution as a function of position along B-scan trace vs. time) of wrapped phase data and corresponding deformation maps in selected regions of the RNF layer (RNFL) over the course of several cardiac cycles. A reproducible phase pattern is seen at each heartbeat cycle for all imaged volunteers. RNF tissue deformations near arteries and veins up to ∼ 1.6 µm were obtained with an average precision for a single pixel of about 30 nm. Differences of motion induced by arteries and veins are also investigated. |
format | Online Article Text |
id | pubmed-8606136 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-86061362021-12-01 Temporal phase evolution OCT for measurement of tissue deformation in the human retina in-vivo Desissaire, Sylvia Schwarzhans, Florian Steiner, Stefan Vass, Clemens Fischer, Georg Pircher, Michael Hitzenberger, Christoph K. Biomed Opt Express Article We demonstrate the use of temporal phase evolution (TPE-) OCT methods to evaluate retinal tissue deformation in-vivo over time periods of several seconds. A custom built spectral domain (SD)-OCT system with an integrated retinal tracker, ensuring stable imaging with sub-speckle precision, was used for imaging. TPE-OCT measures and images phase differences between an initial reference B-scan and each of the subsequent B-scans of the evaluated temporal sequence. In order to demonstrate the precision and repeatability of the measurements, retinal nerve fiber (RNF) tissue deformations induced by retinal vessels pulsating with the heartbeat were analyzed in several healthy subjects. We show TPE maps (M-scans of phase evolution as a function of position along B-scan trace vs. time) of wrapped phase data and corresponding deformation maps in selected regions of the RNF layer (RNFL) over the course of several cardiac cycles. A reproducible phase pattern is seen at each heartbeat cycle for all imaged volunteers. RNF tissue deformations near arteries and veins up to ∼ 1.6 µm were obtained with an average precision for a single pixel of about 30 nm. Differences of motion induced by arteries and veins are also investigated. Optical Society of America 2021-10-25 /pmc/articles/PMC8606136/ /pubmed/34858702 http://dx.doi.org/10.1364/BOE.440893 Text en Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Desissaire, Sylvia Schwarzhans, Florian Steiner, Stefan Vass, Clemens Fischer, Georg Pircher, Michael Hitzenberger, Christoph K. Temporal phase evolution OCT for measurement of tissue deformation in the human retina in-vivo |
title | Temporal phase evolution OCT for measurement of tissue
deformation in the human retina
in-vivo |
title_full | Temporal phase evolution OCT for measurement of tissue
deformation in the human retina
in-vivo |
title_fullStr | Temporal phase evolution OCT for measurement of tissue
deformation in the human retina
in-vivo |
title_full_unstemmed | Temporal phase evolution OCT for measurement of tissue
deformation in the human retina
in-vivo |
title_short | Temporal phase evolution OCT for measurement of tissue
deformation in the human retina
in-vivo |
title_sort | temporal phase evolution oct for measurement of tissue
deformation in the human retina
in-vivo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606136/ https://www.ncbi.nlm.nih.gov/pubmed/34858702 http://dx.doi.org/10.1364/BOE.440893 |
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