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Temporal phase evolution OCT for measurement of tissue deformation in the human retina in-vivo

We demonstrate the use of temporal phase evolution (TPE-) OCT methods to evaluate retinal tissue deformation in-vivo over time periods of several seconds. A custom built spectral domain (SD)-OCT system with an integrated retinal tracker, ensuring stable imaging with sub-speckle precision, was used f...

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Autores principales: Desissaire, Sylvia, Schwarzhans, Florian, Steiner, Stefan, Vass, Clemens, Fischer, Georg, Pircher, Michael, Hitzenberger, Christoph K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606136/
https://www.ncbi.nlm.nih.gov/pubmed/34858702
http://dx.doi.org/10.1364/BOE.440893
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author Desissaire, Sylvia
Schwarzhans, Florian
Steiner, Stefan
Vass, Clemens
Fischer, Georg
Pircher, Michael
Hitzenberger, Christoph K.
author_facet Desissaire, Sylvia
Schwarzhans, Florian
Steiner, Stefan
Vass, Clemens
Fischer, Georg
Pircher, Michael
Hitzenberger, Christoph K.
author_sort Desissaire, Sylvia
collection PubMed
description We demonstrate the use of temporal phase evolution (TPE-) OCT methods to evaluate retinal tissue deformation in-vivo over time periods of several seconds. A custom built spectral domain (SD)-OCT system with an integrated retinal tracker, ensuring stable imaging with sub-speckle precision, was used for imaging. TPE-OCT measures and images phase differences between an initial reference B-scan and each of the subsequent B-scans of the evaluated temporal sequence. In order to demonstrate the precision and repeatability of the measurements, retinal nerve fiber (RNF) tissue deformations induced by retinal vessels pulsating with the heartbeat were analyzed in several healthy subjects. We show TPE maps (M-scans of phase evolution as a function of position along B-scan trace vs. time) of wrapped phase data and corresponding deformation maps in selected regions of the RNF layer (RNFL) over the course of several cardiac cycles. A reproducible phase pattern is seen at each heartbeat cycle for all imaged volunteers. RNF tissue deformations near arteries and veins up to ∼ 1.6 µm were obtained with an average precision for a single pixel of about 30 nm. Differences of motion induced by arteries and veins are also investigated.
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spelling pubmed-86061362021-12-01 Temporal phase evolution OCT for measurement of tissue deformation in the human retina in-vivo Desissaire, Sylvia Schwarzhans, Florian Steiner, Stefan Vass, Clemens Fischer, Georg Pircher, Michael Hitzenberger, Christoph K. Biomed Opt Express Article We demonstrate the use of temporal phase evolution (TPE-) OCT methods to evaluate retinal tissue deformation in-vivo over time periods of several seconds. A custom built spectral domain (SD)-OCT system with an integrated retinal tracker, ensuring stable imaging with sub-speckle precision, was used for imaging. TPE-OCT measures and images phase differences between an initial reference B-scan and each of the subsequent B-scans of the evaluated temporal sequence. In order to demonstrate the precision and repeatability of the measurements, retinal nerve fiber (RNF) tissue deformations induced by retinal vessels pulsating with the heartbeat were analyzed in several healthy subjects. We show TPE maps (M-scans of phase evolution as a function of position along B-scan trace vs. time) of wrapped phase data and corresponding deformation maps in selected regions of the RNF layer (RNFL) over the course of several cardiac cycles. A reproducible phase pattern is seen at each heartbeat cycle for all imaged volunteers. RNF tissue deformations near arteries and veins up to ∼ 1.6 µm were obtained with an average precision for a single pixel of about 30 nm. Differences of motion induced by arteries and veins are also investigated. Optical Society of America 2021-10-25 /pmc/articles/PMC8606136/ /pubmed/34858702 http://dx.doi.org/10.1364/BOE.440893 Text en Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Desissaire, Sylvia
Schwarzhans, Florian
Steiner, Stefan
Vass, Clemens
Fischer, Georg
Pircher, Michael
Hitzenberger, Christoph K.
Temporal phase evolution OCT for measurement of tissue deformation in the human retina in-vivo
title Temporal phase evolution OCT for measurement of tissue deformation in the human retina in-vivo
title_full Temporal phase evolution OCT for measurement of tissue deformation in the human retina in-vivo
title_fullStr Temporal phase evolution OCT for measurement of tissue deformation in the human retina in-vivo
title_full_unstemmed Temporal phase evolution OCT for measurement of tissue deformation in the human retina in-vivo
title_short Temporal phase evolution OCT for measurement of tissue deformation in the human retina in-vivo
title_sort temporal phase evolution oct for measurement of tissue deformation in the human retina in-vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606136/
https://www.ncbi.nlm.nih.gov/pubmed/34858702
http://dx.doi.org/10.1364/BOE.440893
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