Cargando…

Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis

Liquid crystals (LCs) are appealing biomaterials for applications in bone regenerative medicine due to their tunable physical properties and anisotropic viscoelastic behavior. This study reports a novel composite poly (L-lactide) (PLLA) scaffold that is manufactured by a simple electrospinning and b...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhan, Yi, Deng, Bing, Wu, Huixian, Xu, Changpeng, Wang, Ruiying, Li, Wenqiang, Pan, Zhixiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606401/
https://www.ncbi.nlm.nih.gov/pubmed/34819856
http://dx.doi.org/10.3389/fphar.2021.736301
_version_ 1784602330386137088
author Zhan, Yi
Deng, Bing
Wu, Huixian
Xu, Changpeng
Wang, Ruiying
Li, Wenqiang
Pan, Zhixiong
author_facet Zhan, Yi
Deng, Bing
Wu, Huixian
Xu, Changpeng
Wang, Ruiying
Li, Wenqiang
Pan, Zhixiong
author_sort Zhan, Yi
collection PubMed
description Liquid crystals (LCs) are appealing biomaterials for applications in bone regenerative medicine due to their tunable physical properties and anisotropic viscoelastic behavior. This study reports a novel composite poly (L-lactide) (PLLA) scaffold that is manufactured by a simple electrospinning and biomineralization technique that precisely controls the fibrous structure in liquid LC phase. The enriched-LC composites have superior mineralization ability than neat PLLA; furthermore BMSC cells were inoculated onto the HAP-PLLA/LC with hydroxyapatite (HAP) composite scaffold to test the capability for osteogenesis in vitro. The results show that the PLLA/LC with HAP produced by mineralization leads to better cell compatibility, which is beneficial to cell proliferation, osteogenic differentiation, and expression of the angiogenic CD31 gene. Moreover, in vivo studies showed that the HAP-PLLA/LC scaffold with a bone-like environment significantly accelerates new and mature lamellar bone formation by development of a microenvironment for vascularized bone regeneration. Thus, this bionic composite scaffold in an LC state combining osteogenesis with vascularized activities is a promising biomaterial for bone regeneration in defective areas.
format Online
Article
Text
id pubmed-8606401
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-86064012021-11-23 Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis Zhan, Yi Deng, Bing Wu, Huixian Xu, Changpeng Wang, Ruiying Li, Wenqiang Pan, Zhixiong Front Pharmacol Pharmacology Liquid crystals (LCs) are appealing biomaterials for applications in bone regenerative medicine due to their tunable physical properties and anisotropic viscoelastic behavior. This study reports a novel composite poly (L-lactide) (PLLA) scaffold that is manufactured by a simple electrospinning and biomineralization technique that precisely controls the fibrous structure in liquid LC phase. The enriched-LC composites have superior mineralization ability than neat PLLA; furthermore BMSC cells were inoculated onto the HAP-PLLA/LC with hydroxyapatite (HAP) composite scaffold to test the capability for osteogenesis in vitro. The results show that the PLLA/LC with HAP produced by mineralization leads to better cell compatibility, which is beneficial to cell proliferation, osteogenic differentiation, and expression of the angiogenic CD31 gene. Moreover, in vivo studies showed that the HAP-PLLA/LC scaffold with a bone-like environment significantly accelerates new and mature lamellar bone formation by development of a microenvironment for vascularized bone regeneration. Thus, this bionic composite scaffold in an LC state combining osteogenesis with vascularized activities is a promising biomaterial for bone regeneration in defective areas. Frontiers Media S.A. 2021-11-08 /pmc/articles/PMC8606401/ /pubmed/34819856 http://dx.doi.org/10.3389/fphar.2021.736301 Text en Copyright © 2021 Zhan, Deng, Wu, Xu, Wang, Li and Pan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Zhan, Yi
Deng, Bing
Wu, Huixian
Xu, Changpeng
Wang, Ruiying
Li, Wenqiang
Pan, Zhixiong
Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis
title Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis
title_full Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis
title_fullStr Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis
title_full_unstemmed Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis
title_short Biomineralized Composite Liquid Crystal Fiber Scaffold Promotes Bone Regeneration by Enhancement of Osteogenesis and Angiogenesis
title_sort biomineralized composite liquid crystal fiber scaffold promotes bone regeneration by enhancement of osteogenesis and angiogenesis
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606401/
https://www.ncbi.nlm.nih.gov/pubmed/34819856
http://dx.doi.org/10.3389/fphar.2021.736301
work_keys_str_mv AT zhanyi biomineralizedcompositeliquidcrystalfiberscaffoldpromotesboneregenerationbyenhancementofosteogenesisandangiogenesis
AT dengbing biomineralizedcompositeliquidcrystalfiberscaffoldpromotesboneregenerationbyenhancementofosteogenesisandangiogenesis
AT wuhuixian biomineralizedcompositeliquidcrystalfiberscaffoldpromotesboneregenerationbyenhancementofosteogenesisandangiogenesis
AT xuchangpeng biomineralizedcompositeliquidcrystalfiberscaffoldpromotesboneregenerationbyenhancementofosteogenesisandangiogenesis
AT wangruiying biomineralizedcompositeliquidcrystalfiberscaffoldpromotesboneregenerationbyenhancementofosteogenesisandangiogenesis
AT liwenqiang biomineralizedcompositeliquidcrystalfiberscaffoldpromotesboneregenerationbyenhancementofosteogenesisandangiogenesis
AT panzhixiong biomineralizedcompositeliquidcrystalfiberscaffoldpromotesboneregenerationbyenhancementofosteogenesisandangiogenesis