Cargando…
Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing
The bone marrow microenvironment is composed primarily of immune and stromal cells that play important roles in fracture healing. Although immune cells have been identified in mouse bone marrow, variations in their numbers and type during the fracture healing process remain poorly defined. In this s...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606664/ https://www.ncbi.nlm.nih.gov/pubmed/34819916 http://dx.doi.org/10.3389/fendo.2021.666140 |
_version_ | 1784602380975734784 |
---|---|
author | Zhang, Hao Wang, Renkai Wang, Guangchao Zhang, Bo Wang, Chao Li, Di Ding, Chen Wei, Qiang Fan, Zhenyu Tang, Hao Ji, Fang |
author_facet | Zhang, Hao Wang, Renkai Wang, Guangchao Zhang, Bo Wang, Chao Li, Di Ding, Chen Wei, Qiang Fan, Zhenyu Tang, Hao Ji, Fang |
author_sort | Zhang, Hao |
collection | PubMed |
description | The bone marrow microenvironment is composed primarily of immune and stromal cells that play important roles in fracture healing. Although immune cells have been identified in mouse bone marrow, variations in their numbers and type during the fracture healing process remain poorly defined. In this study, single-cell RNA sequencing was used to identify immune cells in fracture tissues, including neutrophils, monocytes, T cells, B cells, and plasma cells. The number of B cells decreased significantly in the early stage of fracture healing. Furthermore, B cells in mice fracture models decreased significantly during the epiphyseal phase and then gradually returned to normal during the epiphyseal transformation phase of fracture healing. The B-cell pattern was opposite to that of bone formation and resorption activities. Notably, B-cell–derived exosomes inhibited bone homeostasis in fracture healing. In humans, a decrease in the number of B cells during the epiphyseal phase stimulated fracture healing. Then, as the numbers of osteoblasts increased during the callus reconstruction stage, the number of B cells gradually recovered, which reduced additional bone regeneration. Thus, B cells are key regulators of fracture healing and inhibit excessive bone regeneration by producing multiple osteoblast inhibitors. |
format | Online Article Text |
id | pubmed-8606664 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-86066642021-11-23 Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing Zhang, Hao Wang, Renkai Wang, Guangchao Zhang, Bo Wang, Chao Li, Di Ding, Chen Wei, Qiang Fan, Zhenyu Tang, Hao Ji, Fang Front Endocrinol (Lausanne) Endocrinology The bone marrow microenvironment is composed primarily of immune and stromal cells that play important roles in fracture healing. Although immune cells have been identified in mouse bone marrow, variations in their numbers and type during the fracture healing process remain poorly defined. In this study, single-cell RNA sequencing was used to identify immune cells in fracture tissues, including neutrophils, monocytes, T cells, B cells, and plasma cells. The number of B cells decreased significantly in the early stage of fracture healing. Furthermore, B cells in mice fracture models decreased significantly during the epiphyseal phase and then gradually returned to normal during the epiphyseal transformation phase of fracture healing. The B-cell pattern was opposite to that of bone formation and resorption activities. Notably, B-cell–derived exosomes inhibited bone homeostasis in fracture healing. In humans, a decrease in the number of B cells during the epiphyseal phase stimulated fracture healing. Then, as the numbers of osteoblasts increased during the callus reconstruction stage, the number of B cells gradually recovered, which reduced additional bone regeneration. Thus, B cells are key regulators of fracture healing and inhibit excessive bone regeneration by producing multiple osteoblast inhibitors. Frontiers Media S.A. 2021-11-08 /pmc/articles/PMC8606664/ /pubmed/34819916 http://dx.doi.org/10.3389/fendo.2021.666140 Text en Copyright © 2021 Zhang, Wang, Wang, Zhang, Wang, Li, Ding, Wei, Fan, Tang and Ji https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Endocrinology Zhang, Hao Wang, Renkai Wang, Guangchao Zhang, Bo Wang, Chao Li, Di Ding, Chen Wei, Qiang Fan, Zhenyu Tang, Hao Ji, Fang Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing |
title | Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing |
title_full | Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing |
title_fullStr | Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing |
title_full_unstemmed | Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing |
title_short | Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing |
title_sort | single-cell rna sequencing reveals b cells are important regulators in fracture healing |
topic | Endocrinology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606664/ https://www.ncbi.nlm.nih.gov/pubmed/34819916 http://dx.doi.org/10.3389/fendo.2021.666140 |
work_keys_str_mv | AT zhanghao singlecellrnasequencingrevealsbcellsareimportantregulatorsinfracturehealing AT wangrenkai singlecellrnasequencingrevealsbcellsareimportantregulatorsinfracturehealing AT wangguangchao singlecellrnasequencingrevealsbcellsareimportantregulatorsinfracturehealing AT zhangbo singlecellrnasequencingrevealsbcellsareimportantregulatorsinfracturehealing AT wangchao singlecellrnasequencingrevealsbcellsareimportantregulatorsinfracturehealing AT lidi singlecellrnasequencingrevealsbcellsareimportantregulatorsinfracturehealing AT dingchen singlecellrnasequencingrevealsbcellsareimportantregulatorsinfracturehealing AT weiqiang singlecellrnasequencingrevealsbcellsareimportantregulatorsinfracturehealing AT fanzhenyu singlecellrnasequencingrevealsbcellsareimportantregulatorsinfracturehealing AT tanghao singlecellrnasequencingrevealsbcellsareimportantregulatorsinfracturehealing AT jifang singlecellrnasequencingrevealsbcellsareimportantregulatorsinfracturehealing |