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Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex

The wide diversity of cortical inhibitory neuron types populating the cortex allows the assembly of diverse microcircuits and endows these circuits with different computational properties. Thus, characterizing neuronal diversity is fundamental to describe the building blocks of cortical microcircuit...

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Autores principales: Nigro, Maximiliano José, Kirikae, Hinako, Kjelsberg, Kasper, Nair, Rajeevkumar Raveendran, Witter, Menno P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606682/
https://www.ncbi.nlm.nih.gov/pubmed/34819840
http://dx.doi.org/10.3389/fncir.2021.781928
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author Nigro, Maximiliano José
Kirikae, Hinako
Kjelsberg, Kasper
Nair, Rajeevkumar Raveendran
Witter, Menno P.
author_facet Nigro, Maximiliano José
Kirikae, Hinako
Kjelsberg, Kasper
Nair, Rajeevkumar Raveendran
Witter, Menno P.
author_sort Nigro, Maximiliano José
collection PubMed
description The wide diversity of cortical inhibitory neuron types populating the cortex allows the assembly of diverse microcircuits and endows these circuits with different computational properties. Thus, characterizing neuronal diversity is fundamental to describe the building blocks of cortical microcircuits and probe their function. To this purpose, the mouse has emerged as a powerful tool to genetically label and manipulate specific inhibitory cell-types in the mammalian brain. Among these cell-types, the parvalbumin-expressing interneuron type (PV-INs) is perhaps the most characterized. Several mouse lines have been generated to target PV-INs. Among these mouse lines, the PV-IRES-Cre lines is the most widely used and demonstrated a high specificity and efficiency in targeting PV-INs in different cortical areas. However, a characterization of the performance across cortical regions is still missing. Here we show that the PV-IRES-Cre mouse line labels only a fraction of PV immunoreactive neurons in perirhinal cortex and other association areas. Our results point to a yet uncharacterized diversity within the PV-INs and emphasize the need to characterize these tools in specific cortical areas.
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spelling pubmed-86066822021-11-23 Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex Nigro, Maximiliano José Kirikae, Hinako Kjelsberg, Kasper Nair, Rajeevkumar Raveendran Witter, Menno P. Front Neural Circuits Neural Circuits The wide diversity of cortical inhibitory neuron types populating the cortex allows the assembly of diverse microcircuits and endows these circuits with different computational properties. Thus, characterizing neuronal diversity is fundamental to describe the building blocks of cortical microcircuits and probe their function. To this purpose, the mouse has emerged as a powerful tool to genetically label and manipulate specific inhibitory cell-types in the mammalian brain. Among these cell-types, the parvalbumin-expressing interneuron type (PV-INs) is perhaps the most characterized. Several mouse lines have been generated to target PV-INs. Among these mouse lines, the PV-IRES-Cre lines is the most widely used and demonstrated a high specificity and efficiency in targeting PV-INs in different cortical areas. However, a characterization of the performance across cortical regions is still missing. Here we show that the PV-IRES-Cre mouse line labels only a fraction of PV immunoreactive neurons in perirhinal cortex and other association areas. Our results point to a yet uncharacterized diversity within the PV-INs and emphasize the need to characterize these tools in specific cortical areas. Frontiers Media S.A. 2021-11-08 /pmc/articles/PMC8606682/ /pubmed/34819840 http://dx.doi.org/10.3389/fncir.2021.781928 Text en Copyright © 2021 Nigro, Kirikae, Kjelsberg, Nair and Witter. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neural Circuits
Nigro, Maximiliano José
Kirikae, Hinako
Kjelsberg, Kasper
Nair, Rajeevkumar Raveendran
Witter, Menno P.
Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex
title Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex
title_full Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex
title_fullStr Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex
title_full_unstemmed Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex
title_short Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows Low Efficiency of Labeling of Parvalbumin Interneurons in the Perirhinal Cortex
title_sort not all that is gold glitters: pv-ires-cre mouse line shows low efficiency of labeling of parvalbumin interneurons in the perirhinal cortex
topic Neural Circuits
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606682/
https://www.ncbi.nlm.nih.gov/pubmed/34819840
http://dx.doi.org/10.3389/fncir.2021.781928
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