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Hydrashift 2/4 daratumumab检测消除达雷妥尤单抗对血清免疫固定电泳干扰的应用
OBJECTIVE: To investigate the interference of daratumumab on immunofixation electrophoresis after treating plasma cell diseases and methods to eliminate the interference. METHODS: Serum samples of eight patients with plasma cell diseases treated with daratumumab in Peking University People's Ho...
Formato: | Online Artículo Texto |
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Lenguaje: | English |
Publicado: |
Editorial office of Chinese Journal of Hematology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8607023/ https://www.ncbi.nlm.nih.gov/pubmed/34788924 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2021.10.008 |
Sumario: | OBJECTIVE: To investigate the interference of daratumumab on immunofixation electrophoresis after treating plasma cell diseases and methods to eliminate the interference. METHODS: Serum samples of eight patients with plasma cell diseases treated with daratumumab in Peking University People's Hospital from April 2020 to March 2021 were collected for standard immunofixation electrophoresis and Hydrashift 2/4 daratumumab assay. RESULTS: After treatment, 81.3% (13/16) of the samples showed drug-induced monoclonal antibodies (IgG-κ). The samples without drug-induced monoclonal bands were related to individual differences, administration intervals, and immunoglobulin levels. Among the samples with IgG-κ monoclonal bands, 76.9% (10/13) could be directly identified as endogenous or exogenous monoclonal bands by immunofixation electrophoresis, and the others (3/13) could be identified by Hydrashift 2/4 daratumumab assay. CONCLUSION: Hydrashift 2/4 daratumumab assay can remove the band of daratumumab on the immunofixation electrophoresis and help with efficacy evaluation. |
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