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Hydrashift 2/4 daratumumab检测消除达雷妥尤单抗对血清免疫固定电泳干扰的应用

OBJECTIVE: To investigate the interference of daratumumab on immunofixation electrophoresis after treating plasma cell diseases and methods to eliminate the interference. METHODS: Serum samples of eight patients with plasma cell diseases treated with daratumumab in Peking University People's Ho...

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Detalles Bibliográficos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Editorial office of Chinese Journal of Hematology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8607023/
https://www.ncbi.nlm.nih.gov/pubmed/34788924
http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2021.10.008
Descripción
Sumario:OBJECTIVE: To investigate the interference of daratumumab on immunofixation electrophoresis after treating plasma cell diseases and methods to eliminate the interference. METHODS: Serum samples of eight patients with plasma cell diseases treated with daratumumab in Peking University People's Hospital from April 2020 to March 2021 were collected for standard immunofixation electrophoresis and Hydrashift 2/4 daratumumab assay. RESULTS: After treatment, 81.3% (13/16) of the samples showed drug-induced monoclonal antibodies (IgG-κ). The samples without drug-induced monoclonal bands were related to individual differences, administration intervals, and immunoglobulin levels. Among the samples with IgG-κ monoclonal bands, 76.9% (10/13) could be directly identified as endogenous or exogenous monoclonal bands by immunofixation electrophoresis, and the others (3/13) could be identified by Hydrashift 2/4 daratumumab assay. CONCLUSION: Hydrashift 2/4 daratumumab assay can remove the band of daratumumab on the immunofixation electrophoresis and help with efficacy evaluation.