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Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy

At the end of the first larval stage, the nematode Caenorhabditis elegans developing in harsh environmental conditions is able to choose an alternative developmental path called the dauer diapause. Dauer larvae exhibit different physiology and behaviors from non-dauer larvae. Using focused ion beam-...

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Autores principales: Britz, Sebastian, Markert, Sebastian Matthias, Witvliet, Daniel, Steyer, Anna Maria, Tröger, Sarah, Mulcahy, Ben, Kollmannsberger, Philip, Schwab, Yannick, Zhen, Mei, Stigloher, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8607169/
https://www.ncbi.nlm.nih.gov/pubmed/34819841
http://dx.doi.org/10.3389/fnana.2021.732520
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author Britz, Sebastian
Markert, Sebastian Matthias
Witvliet, Daniel
Steyer, Anna Maria
Tröger, Sarah
Mulcahy, Ben
Kollmannsberger, Philip
Schwab, Yannick
Zhen, Mei
Stigloher, Christian
author_facet Britz, Sebastian
Markert, Sebastian Matthias
Witvliet, Daniel
Steyer, Anna Maria
Tröger, Sarah
Mulcahy, Ben
Kollmannsberger, Philip
Schwab, Yannick
Zhen, Mei
Stigloher, Christian
author_sort Britz, Sebastian
collection PubMed
description At the end of the first larval stage, the nematode Caenorhabditis elegans developing in harsh environmental conditions is able to choose an alternative developmental path called the dauer diapause. Dauer larvae exhibit different physiology and behaviors from non-dauer larvae. Using focused ion beam-scanning electron microscopy (FIB-SEM), we volumetrically reconstructed the anterior sensory apparatus of C. elegans dauer larvae with unprecedented precision. We provide a detailed description of some neurons, focusing on structural details that were unknown or unresolved by previously published studies. They include the following: (1) dauer-specific branches of the IL2 sensory neurons project into the periphery of anterior sensilla and motor or putative sensory neurons at the sub-lateral cords; (2) ciliated endings of URX sensory neurons are supported by both ILso and AMso socket cells near the amphid openings; (3) variability in amphid sensory dendrites among dauers; and (4) somatic RIP interneurons maintain their projection into the pharyngeal nervous system. Our results support the notion that dauer larvae structurally expand their sensory system to facilitate searching for more favorable environments.
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spelling pubmed-86071692021-11-23 Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy Britz, Sebastian Markert, Sebastian Matthias Witvliet, Daniel Steyer, Anna Maria Tröger, Sarah Mulcahy, Ben Kollmannsberger, Philip Schwab, Yannick Zhen, Mei Stigloher, Christian Front Neuroanat Neuroscience At the end of the first larval stage, the nematode Caenorhabditis elegans developing in harsh environmental conditions is able to choose an alternative developmental path called the dauer diapause. Dauer larvae exhibit different physiology and behaviors from non-dauer larvae. Using focused ion beam-scanning electron microscopy (FIB-SEM), we volumetrically reconstructed the anterior sensory apparatus of C. elegans dauer larvae with unprecedented precision. We provide a detailed description of some neurons, focusing on structural details that were unknown or unresolved by previously published studies. They include the following: (1) dauer-specific branches of the IL2 sensory neurons project into the periphery of anterior sensilla and motor or putative sensory neurons at the sub-lateral cords; (2) ciliated endings of URX sensory neurons are supported by both ILso and AMso socket cells near the amphid openings; (3) variability in amphid sensory dendrites among dauers; and (4) somatic RIP interneurons maintain their projection into the pharyngeal nervous system. Our results support the notion that dauer larvae structurally expand their sensory system to facilitate searching for more favorable environments. Frontiers Media S.A. 2021-11-05 /pmc/articles/PMC8607169/ /pubmed/34819841 http://dx.doi.org/10.3389/fnana.2021.732520 Text en Copyright © 2021 Britz, Markert, Witvliet, Steyer, Tröger, Mulcahy, Kollmannsberger, Schwab, Zhen and Stigloher. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Britz, Sebastian
Markert, Sebastian Matthias
Witvliet, Daniel
Steyer, Anna Maria
Tröger, Sarah
Mulcahy, Ben
Kollmannsberger, Philip
Schwab, Yannick
Zhen, Mei
Stigloher, Christian
Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy
title Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy
title_full Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy
title_fullStr Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy
title_full_unstemmed Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy
title_short Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy
title_sort structural analysis of the caenorhabditis elegans dauer larval anterior sensilla by focused ion beam-scanning electron microscopy
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8607169/
https://www.ncbi.nlm.nih.gov/pubmed/34819841
http://dx.doi.org/10.3389/fnana.2021.732520
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