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Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice

Inhibin (INH) and anti-Müllerian hormone (AMH) are essential in ovarian folliculogenesis and play an inhibitory role in mammalian fertility. However, the interactive effect of INH and AMH on the animal reproduction remains unknown. This study aimed to determine the possible interaction and synergy b...

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Autores principales: Yu, Xue, Qiao, Tong, Hua, Liping, Liu, Shuanghang, Zhao, Xinzhe, Lv, Ce, Zhao, Xuhong, Wang, Jing, Han, Li, Yang, Liguo, Liang, Aixin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8607300/
https://www.ncbi.nlm.nih.gov/pubmed/34820437
http://dx.doi.org/10.3389/fvets.2021.747619
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author Yu, Xue
Qiao, Tong
Hua, Liping
Liu, Shuanghang
Zhao, Xinzhe
Lv, Ce
Zhao, Xuhong
Wang, Jing
Han, Li
Yang, Liguo
Liang, Aixin
author_facet Yu, Xue
Qiao, Tong
Hua, Liping
Liu, Shuanghang
Zhao, Xinzhe
Lv, Ce
Zhao, Xuhong
Wang, Jing
Han, Li
Yang, Liguo
Liang, Aixin
author_sort Yu, Xue
collection PubMed
description Inhibin (INH) and anti-Müllerian hormone (AMH) are essential in ovarian folliculogenesis and play an inhibitory role in mammalian fertility. However, the interactive effect of INH and AMH on the animal reproduction remains unknown. This study aimed to determine the possible interaction and synergy between INH and AMH in steroidogenesis by primary granulosa cells, and investigate their synergistic effect on fertility in mice. In in vitro granulosa cell culture system, we found that the treatment of either INHA or AMH had no significant effect on basal estradiol and progesterone production, whereas both significantly attenuated FSH-induced steroid hormone secretion. Importantly, combined treatment with INHA and AMH showed additive inhibitory effect on FSH-induced estradiol and progesterone production, accompanying a significant downregulation in the expression of FSH-stimulated CYP19A1, HSD3B, CYP11A1, StAR transcripts. The interrelationship of INH and AMH combinations was further investigated through active immune neutralization strategy. Female mice were immunized against INH and AMH eukaryotic expression plasmids, and the litter size was recorded after successfully mating. We observed that both INH and AMH plasmids were able to induce either anti-AMH or anti-INH antibodies in the immunized mice. In comparison with the control group, co-immunization with INH and AMH plasmids induced higher levels of estradiol, resulting in more litter size. Moreover, there was no significant difference on the offspring's weight between each group. Collectively, the results of the present study suggest that INH and AMH have synergistic effect in regulating steroidogenesis and the litter size in mice.
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spelling pubmed-86073002021-11-23 Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice Yu, Xue Qiao, Tong Hua, Liping Liu, Shuanghang Zhao, Xinzhe Lv, Ce Zhao, Xuhong Wang, Jing Han, Li Yang, Liguo Liang, Aixin Front Vet Sci Veterinary Science Inhibin (INH) and anti-Müllerian hormone (AMH) are essential in ovarian folliculogenesis and play an inhibitory role in mammalian fertility. However, the interactive effect of INH and AMH on the animal reproduction remains unknown. This study aimed to determine the possible interaction and synergy between INH and AMH in steroidogenesis by primary granulosa cells, and investigate their synergistic effect on fertility in mice. In in vitro granulosa cell culture system, we found that the treatment of either INHA or AMH had no significant effect on basal estradiol and progesterone production, whereas both significantly attenuated FSH-induced steroid hormone secretion. Importantly, combined treatment with INHA and AMH showed additive inhibitory effect on FSH-induced estradiol and progesterone production, accompanying a significant downregulation in the expression of FSH-stimulated CYP19A1, HSD3B, CYP11A1, StAR transcripts. The interrelationship of INH and AMH combinations was further investigated through active immune neutralization strategy. Female mice were immunized against INH and AMH eukaryotic expression plasmids, and the litter size was recorded after successfully mating. We observed that both INH and AMH plasmids were able to induce either anti-AMH or anti-INH antibodies in the immunized mice. In comparison with the control group, co-immunization with INH and AMH plasmids induced higher levels of estradiol, resulting in more litter size. Moreover, there was no significant difference on the offspring's weight between each group. Collectively, the results of the present study suggest that INH and AMH have synergistic effect in regulating steroidogenesis and the litter size in mice. Frontiers Media S.A. 2021-10-28 /pmc/articles/PMC8607300/ /pubmed/34820437 http://dx.doi.org/10.3389/fvets.2021.747619 Text en Copyright © 2021 Yu, Qiao, Hua, Liu, Zhao, Lv, Zhao, Wang, Han, Yang and Liang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Yu, Xue
Qiao, Tong
Hua, Liping
Liu, Shuanghang
Zhao, Xinzhe
Lv, Ce
Zhao, Xuhong
Wang, Jing
Han, Li
Yang, Liguo
Liang, Aixin
Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice
title Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice
title_full Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice
title_fullStr Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice
title_full_unstemmed Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice
title_short Synergistic Regulatory Effect of Inhibin and Anti-Müllerian Hormone on Fertility of Mice
title_sort synergistic regulatory effect of inhibin and anti-müllerian hormone on fertility of mice
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8607300/
https://www.ncbi.nlm.nih.gov/pubmed/34820437
http://dx.doi.org/10.3389/fvets.2021.747619
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