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MicroRNA-337-3p impedes breast cancer progression by targeting cyclin-dependent kinase 1
MicroRNAs (miRNAs/miRs) function as key regulators in breast cancer (BC). The present study aimed to verify the function and molecular regulation of miR-337-3p in BC cells. Bioinformatics analysis was performed to screen key genes and miRNAs associated with BC. Reverse transcription-quantitative PCR...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8607341/ https://www.ncbi.nlm.nih.gov/pubmed/34820014 http://dx.doi.org/10.3892/ol.2021.13133 |
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author | Kong, Shuxin Liu, Jianyang Zhang, Bin Lv, Feng Yu, Yang Qin, Tao |
author_facet | Kong, Shuxin Liu, Jianyang Zhang, Bin Lv, Feng Yu, Yang Qin, Tao |
author_sort | Kong, Shuxin |
collection | PubMed |
description | MicroRNAs (miRNAs/miRs) function as key regulators in breast cancer (BC). The present study aimed to verify the function and molecular regulation of miR-337-3p in BC cells. Bioinformatics analysis was performed to screen key genes and miRNAs associated with BC. Reverse transcription-quantitative PCR and western blot analyses were performed to detect RNA and protein expression levels. Cell Counting Kit-8, BrdU and cell adhesion assays, and flow cytometric analysis were performed to assess the biological behaviors of BC cells. The dual-luciferase reporter, RNA pull-down assays, and Pearson's correlation analysis were performed to determine the association between miRNAs and mRNAs. Bioinformatics analysis revealed that miR-337-3p and cyclin-dependent kinase 1 (CDK1) acted as key regulators in BC. In addition, miR-337-3p was expressed at low levels in BC cells and tissues, which suppressed BC progression. CDK1 expression was upregulated in BC cells and tissues, which was associated with increased cell proliferation and adhesion, as well as decreased apoptosis in BC. Notably, miR-337-3p targeted CDK1 to inhibit BC cell progression. Taken together, the results of the present study suggest that miR-337-3p plays a tumor-suppressive role in BC by targeting CDK1. |
format | Online Article Text |
id | pubmed-8607341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-86073412021-11-23 MicroRNA-337-3p impedes breast cancer progression by targeting cyclin-dependent kinase 1 Kong, Shuxin Liu, Jianyang Zhang, Bin Lv, Feng Yu, Yang Qin, Tao Oncol Lett Articles MicroRNAs (miRNAs/miRs) function as key regulators in breast cancer (BC). The present study aimed to verify the function and molecular regulation of miR-337-3p in BC cells. Bioinformatics analysis was performed to screen key genes and miRNAs associated with BC. Reverse transcription-quantitative PCR and western blot analyses were performed to detect RNA and protein expression levels. Cell Counting Kit-8, BrdU and cell adhesion assays, and flow cytometric analysis were performed to assess the biological behaviors of BC cells. The dual-luciferase reporter, RNA pull-down assays, and Pearson's correlation analysis were performed to determine the association between miRNAs and mRNAs. Bioinformatics analysis revealed that miR-337-3p and cyclin-dependent kinase 1 (CDK1) acted as key regulators in BC. In addition, miR-337-3p was expressed at low levels in BC cells and tissues, which suppressed BC progression. CDK1 expression was upregulated in BC cells and tissues, which was associated with increased cell proliferation and adhesion, as well as decreased apoptosis in BC. Notably, miR-337-3p targeted CDK1 to inhibit BC cell progression. Taken together, the results of the present study suggest that miR-337-3p plays a tumor-suppressive role in BC by targeting CDK1. D.A. Spandidos 2022-01 2021-11-12 /pmc/articles/PMC8607341/ /pubmed/34820014 http://dx.doi.org/10.3892/ol.2021.13133 Text en Copyright: © Kong et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Kong, Shuxin Liu, Jianyang Zhang, Bin Lv, Feng Yu, Yang Qin, Tao MicroRNA-337-3p impedes breast cancer progression by targeting cyclin-dependent kinase 1 |
title | MicroRNA-337-3p impedes breast cancer progression by targeting cyclin-dependent kinase 1 |
title_full | MicroRNA-337-3p impedes breast cancer progression by targeting cyclin-dependent kinase 1 |
title_fullStr | MicroRNA-337-3p impedes breast cancer progression by targeting cyclin-dependent kinase 1 |
title_full_unstemmed | MicroRNA-337-3p impedes breast cancer progression by targeting cyclin-dependent kinase 1 |
title_short | MicroRNA-337-3p impedes breast cancer progression by targeting cyclin-dependent kinase 1 |
title_sort | microrna-337-3p impedes breast cancer progression by targeting cyclin-dependent kinase 1 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8607341/ https://www.ncbi.nlm.nih.gov/pubmed/34820014 http://dx.doi.org/10.3892/ol.2021.13133 |
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