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Generation of auxin inducible degron (AID) knock-in cell lines for targeted protein degradation in mammalian cells

Targeted protein degradation using degrons, such as the mini-Auxin-inducible degron (mAID), has an advantage over genetic silencing/knockout. However, the efficiency of sgRNA, homologous recombination, tedious expansion, and screening single clones makes the process of tagging endogenous proteins lo...

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Detalles Bibliográficos
Autores principales:	Adhikari, Bikash, Narain, Ashwin, Wolf, Elmar
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8609061/ https://www.ncbi.nlm.nih.gov/pubmed/34849487 http://dx.doi.org/10.1016/j.xpro.2021.100949

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