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Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae
Bacteria orchestrate collective behaviors using the cell-cell communication process called quorum sensing (QS). QS relies on the synthesis, release, and group-wide detection of small molecules called autoinducers. In Vibrio cholerae, a multicellular community aggregation program occurs in liquid, du...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8609355/ https://www.ncbi.nlm.nih.gov/pubmed/34809464 http://dx.doi.org/10.1128/mBio.01518-21 |
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author | Jemielita, Matthew Mashruwala, Ameya A. Valastyan, Julie S. Wingreen, Ned S. Bassler, Bonnie L. |
author_facet | Jemielita, Matthew Mashruwala, Ameya A. Valastyan, Julie S. Wingreen, Ned S. Bassler, Bonnie L. |
author_sort | Jemielita, Matthew |
collection | PubMed |
description | Bacteria orchestrate collective behaviors using the cell-cell communication process called quorum sensing (QS). QS relies on the synthesis, release, and group-wide detection of small molecules called autoinducers. In Vibrio cholerae, a multicellular community aggregation program occurs in liquid, during the stationary phase, and in the high-cell-density QS state. Here, we demonstrate that this aggregation program consists of two subprograms. In one subprogram, which we call void formation, structures form that contain few cells but provide a scaffold within which cells can embed. The other subprogram relies on flagellar machinery and enables cells to enter voids. A genetic screen for factors contributing to void formation, coupled with companion molecular analyses, showed that four extracellular proteases, Vca0812, Vca0813, HapA, and PrtV, control the onset timing of both void formation and aggregation; moreover, proteolytic activity is required. These proteases, or their downstream products, can be shared between void-producing and non-void-forming cells and can elicit aggregation in a normally nonaggregating V. cholerae strain. Employing multiple proteases to control void formation and aggregation timing could provide a redundant and irreversible path to commitment to this community lifestyle. |
format | Online Article Text |
id | pubmed-8609355 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-86093552021-12-02 Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae Jemielita, Matthew Mashruwala, Ameya A. Valastyan, Julie S. Wingreen, Ned S. Bassler, Bonnie L. mBio Research Article Bacteria orchestrate collective behaviors using the cell-cell communication process called quorum sensing (QS). QS relies on the synthesis, release, and group-wide detection of small molecules called autoinducers. In Vibrio cholerae, a multicellular community aggregation program occurs in liquid, during the stationary phase, and in the high-cell-density QS state. Here, we demonstrate that this aggregation program consists of two subprograms. In one subprogram, which we call void formation, structures form that contain few cells but provide a scaffold within which cells can embed. The other subprogram relies on flagellar machinery and enables cells to enter voids. A genetic screen for factors contributing to void formation, coupled with companion molecular analyses, showed that four extracellular proteases, Vca0812, Vca0813, HapA, and PrtV, control the onset timing of both void formation and aggregation; moreover, proteolytic activity is required. These proteases, or their downstream products, can be shared between void-producing and non-void-forming cells and can elicit aggregation in a normally nonaggregating V. cholerae strain. Employing multiple proteases to control void formation and aggregation timing could provide a redundant and irreversible path to commitment to this community lifestyle. American Society for Microbiology 2021-11-23 /pmc/articles/PMC8609355/ /pubmed/34809464 http://dx.doi.org/10.1128/mBio.01518-21 Text en Copyright © 2021 Jemielita et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Jemielita, Matthew Mashruwala, Ameya A. Valastyan, Julie S. Wingreen, Ned S. Bassler, Bonnie L. Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae |
title | Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae |
title_full | Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae |
title_fullStr | Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae |
title_full_unstemmed | Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae |
title_short | Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae |
title_sort | secreted proteases control the timing of aggregative community formation in vibrio cholerae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8609355/ https://www.ncbi.nlm.nih.gov/pubmed/34809464 http://dx.doi.org/10.1128/mBio.01518-21 |
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