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Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae

Bacteria orchestrate collective behaviors using the cell-cell communication process called quorum sensing (QS). QS relies on the synthesis, release, and group-wide detection of small molecules called autoinducers. In Vibrio cholerae, a multicellular community aggregation program occurs in liquid, du...

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Detalles Bibliográficos
Autores principales: Jemielita, Matthew, Mashruwala, Ameya A., Valastyan, Julie S., Wingreen, Ned S., Bassler, Bonnie L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8609355/
https://www.ncbi.nlm.nih.gov/pubmed/34809464
http://dx.doi.org/10.1128/mBio.01518-21
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author Jemielita, Matthew
Mashruwala, Ameya A.
Valastyan, Julie S.
Wingreen, Ned S.
Bassler, Bonnie L.
author_facet Jemielita, Matthew
Mashruwala, Ameya A.
Valastyan, Julie S.
Wingreen, Ned S.
Bassler, Bonnie L.
author_sort Jemielita, Matthew
collection PubMed
description Bacteria orchestrate collective behaviors using the cell-cell communication process called quorum sensing (QS). QS relies on the synthesis, release, and group-wide detection of small molecules called autoinducers. In Vibrio cholerae, a multicellular community aggregation program occurs in liquid, during the stationary phase, and in the high-cell-density QS state. Here, we demonstrate that this aggregation program consists of two subprograms. In one subprogram, which we call void formation, structures form that contain few cells but provide a scaffold within which cells can embed. The other subprogram relies on flagellar machinery and enables cells to enter voids. A genetic screen for factors contributing to void formation, coupled with companion molecular analyses, showed that four extracellular proteases, Vca0812, Vca0813, HapA, and PrtV, control the onset timing of both void formation and aggregation; moreover, proteolytic activity is required. These proteases, or their downstream products, can be shared between void-producing and non-void-forming cells and can elicit aggregation in a normally nonaggregating V. cholerae strain. Employing multiple proteases to control void formation and aggregation timing could provide a redundant and irreversible path to commitment to this community lifestyle.
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spelling pubmed-86093552021-12-02 Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae Jemielita, Matthew Mashruwala, Ameya A. Valastyan, Julie S. Wingreen, Ned S. Bassler, Bonnie L. mBio Research Article Bacteria orchestrate collective behaviors using the cell-cell communication process called quorum sensing (QS). QS relies on the synthesis, release, and group-wide detection of small molecules called autoinducers. In Vibrio cholerae, a multicellular community aggregation program occurs in liquid, during the stationary phase, and in the high-cell-density QS state. Here, we demonstrate that this aggregation program consists of two subprograms. In one subprogram, which we call void formation, structures form that contain few cells but provide a scaffold within which cells can embed. The other subprogram relies on flagellar machinery and enables cells to enter voids. A genetic screen for factors contributing to void formation, coupled with companion molecular analyses, showed that four extracellular proteases, Vca0812, Vca0813, HapA, and PrtV, control the onset timing of both void formation and aggregation; moreover, proteolytic activity is required. These proteases, or their downstream products, can be shared between void-producing and non-void-forming cells and can elicit aggregation in a normally nonaggregating V. cholerae strain. Employing multiple proteases to control void formation and aggregation timing could provide a redundant and irreversible path to commitment to this community lifestyle. American Society for Microbiology 2021-11-23 /pmc/articles/PMC8609355/ /pubmed/34809464 http://dx.doi.org/10.1128/mBio.01518-21 Text en Copyright © 2021 Jemielita et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Jemielita, Matthew
Mashruwala, Ameya A.
Valastyan, Julie S.
Wingreen, Ned S.
Bassler, Bonnie L.
Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae
title Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae
title_full Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae
title_fullStr Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae
title_full_unstemmed Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae
title_short Secreted Proteases Control the Timing of Aggregative Community Formation in Vibrio cholerae
title_sort secreted proteases control the timing of aggregative community formation in vibrio cholerae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8609355/
https://www.ncbi.nlm.nih.gov/pubmed/34809464
http://dx.doi.org/10.1128/mBio.01518-21
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