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Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates

Gut microbiota are influenced by factors such as diet, habitat, and social contact, which directly affect the host's health. Studies related to gut microbiota in non-human primates are increasing worldwide. However, little remains known about the gut bacterial composition in wild Brazilian monk...

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Autores principales: Grassotti, Tiela Trapp, Kothe, Caroline Isabel, Prichula, Janira, Mohellibi, Nacer, Mann, Michele Bertoni, Wagner, Paulo Guilherme Carniel, Campos, Fabricio Souza, Campos, Aline Alves Scarpellini, Frazzon, Jeverson, Frazzon, Ana Paula Guedes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8610302/
https://www.ncbi.nlm.nih.gov/pubmed/34841339
http://dx.doi.org/10.1016/j.crmicr.2021.100048
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author Grassotti, Tiela Trapp
Kothe, Caroline Isabel
Prichula, Janira
Mohellibi, Nacer
Mann, Michele Bertoni
Wagner, Paulo Guilherme Carniel
Campos, Fabricio Souza
Campos, Aline Alves Scarpellini
Frazzon, Jeverson
Frazzon, Ana Paula Guedes
author_facet Grassotti, Tiela Trapp
Kothe, Caroline Isabel
Prichula, Janira
Mohellibi, Nacer
Mann, Michele Bertoni
Wagner, Paulo Guilherme Carniel
Campos, Fabricio Souza
Campos, Aline Alves Scarpellini
Frazzon, Jeverson
Frazzon, Ana Paula Guedes
author_sort Grassotti, Tiela Trapp
collection PubMed
description Gut microbiota are influenced by factors such as diet, habitat, and social contact, which directly affect the host's health. Studies related to gut microbiota in non-human primates are increasing worldwide. However, little remains known about the gut bacterial composition in wild Brazilian monkeys. Therefore, we studied the fecal microbiota composition of wild black capuchin monkey (Sapajus nigritus) (n=10) populations from two different Atlantic Forest biome fragments (five individuals per fragment) in south Brazil. The bacterial community was identified via the high-throughput sequencing and partial amplification of the 16S rRNA gene (V4 region) using an Ion Personal Genome Machine (PGM(TM)) System. In contrast to other studies involving monkey microbiota, which have generally reported the phyla Firmicutes and Bacteroidetes as predominant, black capuchin monkeys showed a high relative abundance of Proteobacteria ([Formula: see text] = 80.54%), followed by Firmicutes ([Formula: see text] = 12.14%), Actinobacteria ([Formula: see text] = 4.60%), and Bacteriodetes ([Formula: see text] = 1.31%). This observed particularity may have been influenced by anthropogenic actions related to the wild habitat and/or diet specific to the Brazilian biome's characteristics and/or monkey foraging behavior. Comparisons of species richness (Chao1) and diversity indices (Simpson and InvSimpson) showed no significant differences between the two groups of monkeys. Interestingly, PICRUSt2 analysis revealed that metabolic pathways present in the bacterial communities were associated with xenobiotic biodegradation and the biosynthesis of secondary metabolites, which may suggest positive effects on monkey health and conservation in this anthropogenic habitat. Infectious disease-associated microorganisms were also observed in the samples. The present study provides information about the bacterial population and metabolic functions present in fecal microbiota, which may contribute to a better understanding of the ecology and biology of black capuchin monkeys living in forest fragments within the Atlantic Forest biome in southern Brazil. Additionally, the present study demonstrates that the fecal bacterial communities of wild black capuchin monkeys in this area are divergent from those of other wild non-human primates.
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spelling pubmed-86103022021-11-26 Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates Grassotti, Tiela Trapp Kothe, Caroline Isabel Prichula, Janira Mohellibi, Nacer Mann, Michele Bertoni Wagner, Paulo Guilherme Carniel Campos, Fabricio Souza Campos, Aline Alves Scarpellini Frazzon, Jeverson Frazzon, Ana Paula Guedes Curr Res Microb Sci Research Paper Gut microbiota are influenced by factors such as diet, habitat, and social contact, which directly affect the host's health. Studies related to gut microbiota in non-human primates are increasing worldwide. However, little remains known about the gut bacterial composition in wild Brazilian monkeys. Therefore, we studied the fecal microbiota composition of wild black capuchin monkey (Sapajus nigritus) (n=10) populations from two different Atlantic Forest biome fragments (five individuals per fragment) in south Brazil. The bacterial community was identified via the high-throughput sequencing and partial amplification of the 16S rRNA gene (V4 region) using an Ion Personal Genome Machine (PGM(TM)) System. In contrast to other studies involving monkey microbiota, which have generally reported the phyla Firmicutes and Bacteroidetes as predominant, black capuchin monkeys showed a high relative abundance of Proteobacteria ([Formula: see text] = 80.54%), followed by Firmicutes ([Formula: see text] = 12.14%), Actinobacteria ([Formula: see text] = 4.60%), and Bacteriodetes ([Formula: see text] = 1.31%). This observed particularity may have been influenced by anthropogenic actions related to the wild habitat and/or diet specific to the Brazilian biome's characteristics and/or monkey foraging behavior. Comparisons of species richness (Chao1) and diversity indices (Simpson and InvSimpson) showed no significant differences between the two groups of monkeys. Interestingly, PICRUSt2 analysis revealed that metabolic pathways present in the bacterial communities were associated with xenobiotic biodegradation and the biosynthesis of secondary metabolites, which may suggest positive effects on monkey health and conservation in this anthropogenic habitat. Infectious disease-associated microorganisms were also observed in the samples. The present study provides information about the bacterial population and metabolic functions present in fecal microbiota, which may contribute to a better understanding of the ecology and biology of black capuchin monkeys living in forest fragments within the Atlantic Forest biome in southern Brazil. Additionally, the present study demonstrates that the fecal bacterial communities of wild black capuchin monkeys in this area are divergent from those of other wild non-human primates. Elsevier 2021-07-10 /pmc/articles/PMC8610302/ /pubmed/34841339 http://dx.doi.org/10.1016/j.crmicr.2021.100048 Text en © 2021 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Grassotti, Tiela Trapp
Kothe, Caroline Isabel
Prichula, Janira
Mohellibi, Nacer
Mann, Michele Bertoni
Wagner, Paulo Guilherme Carniel
Campos, Fabricio Souza
Campos, Aline Alves Scarpellini
Frazzon, Jeverson
Frazzon, Ana Paula Guedes
Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates
title Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates
title_full Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates
title_fullStr Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates
title_full_unstemmed Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates
title_short Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates
title_sort fecal bacterial communities of wild black capuchin monkeys (sapajus nigritus) from the atlantic forest biome in southern brazil are divergent from those of other non-human primates
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8610302/
https://www.ncbi.nlm.nih.gov/pubmed/34841339
http://dx.doi.org/10.1016/j.crmicr.2021.100048
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