Cargando…
Identification of colonies of cultured shellfish-associated Arcobacter species by Elastic Light Scatter Analysis
An increasing number of Arcobacter species (including several regarded as emerging human foodborne pathogens) have been isolated from shellfish, an important food commodity. A method to distinguish these species and render viable isolates for further analysis would benefit epidemiological and ecolog...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8610310/ https://www.ncbi.nlm.nih.gov/pubmed/34841324 http://dx.doi.org/10.1016/j.crmicr.2021.100033 |
_version_ | 1784603085884096512 |
---|---|
author | On, Stephen L.W. Miller, William G. Yee, Emma Sturgis, Jennifer Patsekin, Valery Lindsay, James A. Robinson, J. Paul |
author_facet | On, Stephen L.W. Miller, William G. Yee, Emma Sturgis, Jennifer Patsekin, Valery Lindsay, James A. Robinson, J. Paul |
author_sort | On, Stephen L.W. |
collection | PubMed |
description | An increasing number of Arcobacter species (including several regarded as emerging human foodborne pathogens) have been isolated from shellfish, an important food commodity. A method to distinguish these species and render viable isolates for further analysis would benefit epidemiological and ecological studies. We describe a method based on Elastic Light Scatter analysis (ELSA) for the detection and discrimination of eleven shellfish-associated Arcobacter species. Although substantive differences in the growth rates of some taxa were seen, ELSA was able to differentiate all the species studied, apart from some strains of A. butzleri and A. cryaerophilus, which were nonetheless distinguished from all other species examined. ELSA appears to be a promising new approach for the detection and identification of Arcobacter species in shellfish and may also be applicable for studies in other foods and matrices. |
format | Online Article Text |
id | pubmed-8610310 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-86103102021-11-26 Identification of colonies of cultured shellfish-associated Arcobacter species by Elastic Light Scatter Analysis On, Stephen L.W. Miller, William G. Yee, Emma Sturgis, Jennifer Patsekin, Valery Lindsay, James A. Robinson, J. Paul Curr Res Microb Sci Research Paper An increasing number of Arcobacter species (including several regarded as emerging human foodborne pathogens) have been isolated from shellfish, an important food commodity. A method to distinguish these species and render viable isolates for further analysis would benefit epidemiological and ecological studies. We describe a method based on Elastic Light Scatter analysis (ELSA) for the detection and discrimination of eleven shellfish-associated Arcobacter species. Although substantive differences in the growth rates of some taxa were seen, ELSA was able to differentiate all the species studied, apart from some strains of A. butzleri and A. cryaerophilus, which were nonetheless distinguished from all other species examined. ELSA appears to be a promising new approach for the detection and identification of Arcobacter species in shellfish and may also be applicable for studies in other foods and matrices. Elsevier 2021-04-20 /pmc/articles/PMC8610310/ /pubmed/34841324 http://dx.doi.org/10.1016/j.crmicr.2021.100033 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Paper On, Stephen L.W. Miller, William G. Yee, Emma Sturgis, Jennifer Patsekin, Valery Lindsay, James A. Robinson, J. Paul Identification of colonies of cultured shellfish-associated Arcobacter species by Elastic Light Scatter Analysis |
title | Identification of colonies of cultured shellfish-associated Arcobacter species by Elastic Light Scatter Analysis |
title_full | Identification of colonies of cultured shellfish-associated Arcobacter species by Elastic Light Scatter Analysis |
title_fullStr | Identification of colonies of cultured shellfish-associated Arcobacter species by Elastic Light Scatter Analysis |
title_full_unstemmed | Identification of colonies of cultured shellfish-associated Arcobacter species by Elastic Light Scatter Analysis |
title_short | Identification of colonies of cultured shellfish-associated Arcobacter species by Elastic Light Scatter Analysis |
title_sort | identification of colonies of cultured shellfish-associated arcobacter species by elastic light scatter analysis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8610310/ https://www.ncbi.nlm.nih.gov/pubmed/34841324 http://dx.doi.org/10.1016/j.crmicr.2021.100033 |
work_keys_str_mv | AT onstephenlw identificationofcoloniesofculturedshellfishassociatedarcobacterspeciesbyelasticlightscatteranalysis AT millerwilliamg identificationofcoloniesofculturedshellfishassociatedarcobacterspeciesbyelasticlightscatteranalysis AT yeeemma identificationofcoloniesofculturedshellfishassociatedarcobacterspeciesbyelasticlightscatteranalysis AT sturgisjennifer identificationofcoloniesofculturedshellfishassociatedarcobacterspeciesbyelasticlightscatteranalysis AT patsekinvalery identificationofcoloniesofculturedshellfishassociatedarcobacterspeciesbyelasticlightscatteranalysis AT lindsayjamesa identificationofcoloniesofculturedshellfishassociatedarcobacterspeciesbyelasticlightscatteranalysis AT robinsonjpaul identificationofcoloniesofculturedshellfishassociatedarcobacterspeciesbyelasticlightscatteranalysis |