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Isolation and identification of high fluoride resistant bacteria from water samples of Dindigul district, Tamil Nadu, South India

Fluoride (F(−)) pollution is one of the major issues in India and worldwide. Water samples were collected, and analyse the physicochemical characteristics. From the results, acceptable limit pH ranges, low and high electrical conductivity (EC) values, high-level of TDS (total dissolved solids) and c...

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Autores principales: Chellaiah, Edward Raja, Ravi, Pandeeswari, Uthandakalaipandian, Ramesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8610349/
https://www.ncbi.nlm.nih.gov/pubmed/34841329
http://dx.doi.org/10.1016/j.crmicr.2021.100038
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author Chellaiah, Edward Raja
Ravi, Pandeeswari
Uthandakalaipandian, Ramesh
author_facet Chellaiah, Edward Raja
Ravi, Pandeeswari
Uthandakalaipandian, Ramesh
author_sort Chellaiah, Edward Raja
collection PubMed
description Fluoride (F(−)) pollution is one of the major issues in India and worldwide. Water samples were collected, and analyse the physicochemical characteristics. From the results, acceptable limit pH ranges, low and high electrical conductivity (EC) values, high-level of TDS (total dissolved solids) and chloride (Cl(−)) values, less than desirable and higher than permissible F(−) concentrations (4.7 and 11 ppm) were found. At first, ninety-three colonies were screened using rapid hicoliform agar plates. After that, sixty-six F(−) resistant colonies were picked up from 50  mM NaF (Sodium fluoride) containing LB agar plates. Finally, eight isolates were showing a high degree of F(−) resistance (200–-300 mM NaF) and selected for further studies. High F(−) resistance isolates exhibited β and γ-haemolytic activities were determined in blood agar plates. F(−) resistant isolates showed their salt tolerances ranged from 4% to 7% NaCl and resistant to multiple antibiotics. Biochemical and 16S rRNA sequencing results showed that the F(−) resistant isolates were identified as Enterobacter cloacae strain 3, E. hormaechei strain 14, Enterobacter sp. strain 21, E. hormaechei strain 22, E. coli strain S2-9, Aeromonas caviae strain 31, A. caviae strain 32, A. caviae strain 34. All strains were submitted in the NCBI database with the accession numbers (MW131637, MW131639; MW131650-655). The F(−) resistant gene ‘crcB’ gene was successfully amplified from the resistant isolates using gene-specific primers. These results have demonstrated that fluoride resistant bacteria would be useful for bacterial fluoride bioremediation near future.
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spelling pubmed-86103492021-11-26 Isolation and identification of high fluoride resistant bacteria from water samples of Dindigul district, Tamil Nadu, South India Chellaiah, Edward Raja Ravi, Pandeeswari Uthandakalaipandian, Ramesh Curr Res Microb Sci Research Paper Fluoride (F(−)) pollution is one of the major issues in India and worldwide. Water samples were collected, and analyse the physicochemical characteristics. From the results, acceptable limit pH ranges, low and high electrical conductivity (EC) values, high-level of TDS (total dissolved solids) and chloride (Cl(−)) values, less than desirable and higher than permissible F(−) concentrations (4.7 and 11 ppm) were found. At first, ninety-three colonies were screened using rapid hicoliform agar plates. After that, sixty-six F(−) resistant colonies were picked up from 50  mM NaF (Sodium fluoride) containing LB agar plates. Finally, eight isolates were showing a high degree of F(−) resistance (200–-300 mM NaF) and selected for further studies. High F(−) resistance isolates exhibited β and γ-haemolytic activities were determined in blood agar plates. F(−) resistant isolates showed their salt tolerances ranged from 4% to 7% NaCl and resistant to multiple antibiotics. Biochemical and 16S rRNA sequencing results showed that the F(−) resistant isolates were identified as Enterobacter cloacae strain 3, E. hormaechei strain 14, Enterobacter sp. strain 21, E. hormaechei strain 22, E. coli strain S2-9, Aeromonas caviae strain 31, A. caviae strain 32, A. caviae strain 34. All strains were submitted in the NCBI database with the accession numbers (MW131637, MW131639; MW131650-655). The F(−) resistant gene ‘crcB’ gene was successfully amplified from the resistant isolates using gene-specific primers. These results have demonstrated that fluoride resistant bacteria would be useful for bacterial fluoride bioremediation near future. Elsevier 2021-05-12 /pmc/articles/PMC8610349/ /pubmed/34841329 http://dx.doi.org/10.1016/j.crmicr.2021.100038 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Chellaiah, Edward Raja
Ravi, Pandeeswari
Uthandakalaipandian, Ramesh
Isolation and identification of high fluoride resistant bacteria from water samples of Dindigul district, Tamil Nadu, South India
title Isolation and identification of high fluoride resistant bacteria from water samples of Dindigul district, Tamil Nadu, South India
title_full Isolation and identification of high fluoride resistant bacteria from water samples of Dindigul district, Tamil Nadu, South India
title_fullStr Isolation and identification of high fluoride resistant bacteria from water samples of Dindigul district, Tamil Nadu, South India
title_full_unstemmed Isolation and identification of high fluoride resistant bacteria from water samples of Dindigul district, Tamil Nadu, South India
title_short Isolation and identification of high fluoride resistant bacteria from water samples of Dindigul district, Tamil Nadu, South India
title_sort isolation and identification of high fluoride resistant bacteria from water samples of dindigul district, tamil nadu, south india
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8610349/
https://www.ncbi.nlm.nih.gov/pubmed/34841329
http://dx.doi.org/10.1016/j.crmicr.2021.100038
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