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Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes
Osteoarthritis (OA) impacts hundreds of millions of people worldwide, with those affected incurring significant physical and financial burdens. Injuries such as focal defects to the articular surface are a major contributing risk factor for the development of OA. Current cartilage repair strategies...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611001/ https://www.ncbi.nlm.nih.gov/pubmed/34815400 http://dx.doi.org/10.1038/s41536-021-00187-3 |
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author | Petrigliano, Frank A. Liu, Nancy Q. Lee, Siyoung Tassey, Jade Sarkar, Arijita Lin, Yucheng Li, Liangliang Yu, Yifan Geng, Dawei Zhang, Jiankang Shkhyan, Ruzanna Bogdanov, Jacob Van Handel, Ben Ferguson, Gabriel B. Lee, Youngjoo Hinderer, Svenja Tseng, Kuo-Chang Kavanaugh, Aaron Crump, J. Gage Pyle, April D. Schenke-Layland, Katja Billi, Fabrizio Wang, Liming Lieberman, Jay Hurtig, Mark Evseenko, Denis |
author_facet | Petrigliano, Frank A. Liu, Nancy Q. Lee, Siyoung Tassey, Jade Sarkar, Arijita Lin, Yucheng Li, Liangliang Yu, Yifan Geng, Dawei Zhang, Jiankang Shkhyan, Ruzanna Bogdanov, Jacob Van Handel, Ben Ferguson, Gabriel B. Lee, Youngjoo Hinderer, Svenja Tseng, Kuo-Chang Kavanaugh, Aaron Crump, J. Gage Pyle, April D. Schenke-Layland, Katja Billi, Fabrizio Wang, Liming Lieberman, Jay Hurtig, Mark Evseenko, Denis |
author_sort | Petrigliano, Frank A. |
collection | PubMed |
description | Osteoarthritis (OA) impacts hundreds of millions of people worldwide, with those affected incurring significant physical and financial burdens. Injuries such as focal defects to the articular surface are a major contributing risk factor for the development of OA. Current cartilage repair strategies are moderately effective at reducing pain but often replace damaged tissue with biomechanically inferior fibrocartilage. Here we describe the development, transcriptomic ontogenetic characterization and quality assessment at the single cell level, as well as the scaled manufacturing of an allogeneic human pluripotent stem cell-derived articular chondrocyte formulation that exhibits long-term functional repair of porcine articular cartilage. These results define a new potential clinical paradigm for articular cartilage repair and mitigation of the associated risk of OA. |
format | Online Article Text |
id | pubmed-8611001 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-86110012021-12-01 Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes Petrigliano, Frank A. Liu, Nancy Q. Lee, Siyoung Tassey, Jade Sarkar, Arijita Lin, Yucheng Li, Liangliang Yu, Yifan Geng, Dawei Zhang, Jiankang Shkhyan, Ruzanna Bogdanov, Jacob Van Handel, Ben Ferguson, Gabriel B. Lee, Youngjoo Hinderer, Svenja Tseng, Kuo-Chang Kavanaugh, Aaron Crump, J. Gage Pyle, April D. Schenke-Layland, Katja Billi, Fabrizio Wang, Liming Lieberman, Jay Hurtig, Mark Evseenko, Denis NPJ Regen Med Article Osteoarthritis (OA) impacts hundreds of millions of people worldwide, with those affected incurring significant physical and financial burdens. Injuries such as focal defects to the articular surface are a major contributing risk factor for the development of OA. Current cartilage repair strategies are moderately effective at reducing pain but often replace damaged tissue with biomechanically inferior fibrocartilage. Here we describe the development, transcriptomic ontogenetic characterization and quality assessment at the single cell level, as well as the scaled manufacturing of an allogeneic human pluripotent stem cell-derived articular chondrocyte formulation that exhibits long-term functional repair of porcine articular cartilage. These results define a new potential clinical paradigm for articular cartilage repair and mitigation of the associated risk of OA. Nature Publishing Group UK 2021-11-23 /pmc/articles/PMC8611001/ /pubmed/34815400 http://dx.doi.org/10.1038/s41536-021-00187-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Petrigliano, Frank A. Liu, Nancy Q. Lee, Siyoung Tassey, Jade Sarkar, Arijita Lin, Yucheng Li, Liangliang Yu, Yifan Geng, Dawei Zhang, Jiankang Shkhyan, Ruzanna Bogdanov, Jacob Van Handel, Ben Ferguson, Gabriel B. Lee, Youngjoo Hinderer, Svenja Tseng, Kuo-Chang Kavanaugh, Aaron Crump, J. Gage Pyle, April D. Schenke-Layland, Katja Billi, Fabrizio Wang, Liming Lieberman, Jay Hurtig, Mark Evseenko, Denis Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes |
title | Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes |
title_full | Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes |
title_fullStr | Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes |
title_full_unstemmed | Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes |
title_short | Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes |
title_sort | long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611001/ https://www.ncbi.nlm.nih.gov/pubmed/34815400 http://dx.doi.org/10.1038/s41536-021-00187-3 |
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