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The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients

Glioblastoma is a devastating tumor of the central nervous system characterized by a poor survival and an extremely dark prognosis, making its diagnosis, treatment and monitoring highly challenging. Numerous studies have highlighted extracellular vesicles (EVs) as key players of tumor growth, invasi...

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Autores principales: Sabbagh, Quentin, André-Grégoire, Gwennan, Alves-Nicolau, Carolina, Dupont, Aurélien, Bidère, Nicolas, Jouglar, Emmanuel, Guével, Laëtitia, Frénel, Jean-Sébastien, Gavard, Julie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611030/
https://www.ncbi.nlm.nih.gov/pubmed/34815502
http://dx.doi.org/10.1038/s41598-021-02254-7
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author Sabbagh, Quentin
André-Grégoire, Gwennan
Alves-Nicolau, Carolina
Dupont, Aurélien
Bidère, Nicolas
Jouglar, Emmanuel
Guével, Laëtitia
Frénel, Jean-Sébastien
Gavard, Julie
author_facet Sabbagh, Quentin
André-Grégoire, Gwennan
Alves-Nicolau, Carolina
Dupont, Aurélien
Bidère, Nicolas
Jouglar, Emmanuel
Guével, Laëtitia
Frénel, Jean-Sébastien
Gavard, Julie
author_sort Sabbagh, Quentin
collection PubMed
description Glioblastoma is a devastating tumor of the central nervous system characterized by a poor survival and an extremely dark prognosis, making its diagnosis, treatment and monitoring highly challenging. Numerous studies have highlighted extracellular vesicles (EVs) as key players of tumor growth, invasiveness and resistance, as they carry and disseminate oncogenic material in the local tumor microenvironment and at distance. However, whether their quality and quantity reflect individual health status and changes in homeostasis is still not fully elucidated. Here, we separated EVs from plasma collected at different time points alongside with the clinical management of GBM patients. Our findings confirm that plasmatic EVs could be separated and characterized with standardized protocols, thereby ensuring the reliability of measuring vesiclemia, i.e. extracellular vesicle concentration in plasma. This unveils that vesiclemia is a dynamic parameter, which could be reflecting tumor burden and/or response to treatments. Further label-free liquid chromatography tandem mass spectrometry unmasks the von Willebrand Factor (VWF) as a selective protein hallmark for GBM-patient EVs. Our data thus support the notion that EVs from GBM patients showed differential protein cargos that can be further surveyed in circulating EVs, together with vesiclemia.
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spelling pubmed-86110302021-11-26 The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients Sabbagh, Quentin André-Grégoire, Gwennan Alves-Nicolau, Carolina Dupont, Aurélien Bidère, Nicolas Jouglar, Emmanuel Guével, Laëtitia Frénel, Jean-Sébastien Gavard, Julie Sci Rep Article Glioblastoma is a devastating tumor of the central nervous system characterized by a poor survival and an extremely dark prognosis, making its diagnosis, treatment and monitoring highly challenging. Numerous studies have highlighted extracellular vesicles (EVs) as key players of tumor growth, invasiveness and resistance, as they carry and disseminate oncogenic material in the local tumor microenvironment and at distance. However, whether their quality and quantity reflect individual health status and changes in homeostasis is still not fully elucidated. Here, we separated EVs from plasma collected at different time points alongside with the clinical management of GBM patients. Our findings confirm that plasmatic EVs could be separated and characterized with standardized protocols, thereby ensuring the reliability of measuring vesiclemia, i.e. extracellular vesicle concentration in plasma. This unveils that vesiclemia is a dynamic parameter, which could be reflecting tumor burden and/or response to treatments. Further label-free liquid chromatography tandem mass spectrometry unmasks the von Willebrand Factor (VWF) as a selective protein hallmark for GBM-patient EVs. Our data thus support the notion that EVs from GBM patients showed differential protein cargos that can be further surveyed in circulating EVs, together with vesiclemia. Nature Publishing Group UK 2021-11-23 /pmc/articles/PMC8611030/ /pubmed/34815502 http://dx.doi.org/10.1038/s41598-021-02254-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Sabbagh, Quentin
André-Grégoire, Gwennan
Alves-Nicolau, Carolina
Dupont, Aurélien
Bidère, Nicolas
Jouglar, Emmanuel
Guével, Laëtitia
Frénel, Jean-Sébastien
Gavard, Julie
The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients
title The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients
title_full The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients
title_fullStr The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients
title_full_unstemmed The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients
title_short The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients
title_sort von willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611030/
https://www.ncbi.nlm.nih.gov/pubmed/34815502
http://dx.doi.org/10.1038/s41598-021-02254-7
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