Cargando…
The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients
Glioblastoma is a devastating tumor of the central nervous system characterized by a poor survival and an extremely dark prognosis, making its diagnosis, treatment and monitoring highly challenging. Numerous studies have highlighted extracellular vesicles (EVs) as key players of tumor growth, invasi...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611030/ https://www.ncbi.nlm.nih.gov/pubmed/34815502 http://dx.doi.org/10.1038/s41598-021-02254-7 |
_version_ | 1784603219985432576 |
---|---|
author | Sabbagh, Quentin André-Grégoire, Gwennan Alves-Nicolau, Carolina Dupont, Aurélien Bidère, Nicolas Jouglar, Emmanuel Guével, Laëtitia Frénel, Jean-Sébastien Gavard, Julie |
author_facet | Sabbagh, Quentin André-Grégoire, Gwennan Alves-Nicolau, Carolina Dupont, Aurélien Bidère, Nicolas Jouglar, Emmanuel Guével, Laëtitia Frénel, Jean-Sébastien Gavard, Julie |
author_sort | Sabbagh, Quentin |
collection | PubMed |
description | Glioblastoma is a devastating tumor of the central nervous system characterized by a poor survival and an extremely dark prognosis, making its diagnosis, treatment and monitoring highly challenging. Numerous studies have highlighted extracellular vesicles (EVs) as key players of tumor growth, invasiveness and resistance, as they carry and disseminate oncogenic material in the local tumor microenvironment and at distance. However, whether their quality and quantity reflect individual health status and changes in homeostasis is still not fully elucidated. Here, we separated EVs from plasma collected at different time points alongside with the clinical management of GBM patients. Our findings confirm that plasmatic EVs could be separated and characterized with standardized protocols, thereby ensuring the reliability of measuring vesiclemia, i.e. extracellular vesicle concentration in plasma. This unveils that vesiclemia is a dynamic parameter, which could be reflecting tumor burden and/or response to treatments. Further label-free liquid chromatography tandem mass spectrometry unmasks the von Willebrand Factor (VWF) as a selective protein hallmark for GBM-patient EVs. Our data thus support the notion that EVs from GBM patients showed differential protein cargos that can be further surveyed in circulating EVs, together with vesiclemia. |
format | Online Article Text |
id | pubmed-8611030 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-86110302021-11-26 The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients Sabbagh, Quentin André-Grégoire, Gwennan Alves-Nicolau, Carolina Dupont, Aurélien Bidère, Nicolas Jouglar, Emmanuel Guével, Laëtitia Frénel, Jean-Sébastien Gavard, Julie Sci Rep Article Glioblastoma is a devastating tumor of the central nervous system characterized by a poor survival and an extremely dark prognosis, making its diagnosis, treatment and monitoring highly challenging. Numerous studies have highlighted extracellular vesicles (EVs) as key players of tumor growth, invasiveness and resistance, as they carry and disseminate oncogenic material in the local tumor microenvironment and at distance. However, whether their quality and quantity reflect individual health status and changes in homeostasis is still not fully elucidated. Here, we separated EVs from plasma collected at different time points alongside with the clinical management of GBM patients. Our findings confirm that plasmatic EVs could be separated and characterized with standardized protocols, thereby ensuring the reliability of measuring vesiclemia, i.e. extracellular vesicle concentration in plasma. This unveils that vesiclemia is a dynamic parameter, which could be reflecting tumor burden and/or response to treatments. Further label-free liquid chromatography tandem mass spectrometry unmasks the von Willebrand Factor (VWF) as a selective protein hallmark for GBM-patient EVs. Our data thus support the notion that EVs from GBM patients showed differential protein cargos that can be further surveyed in circulating EVs, together with vesiclemia. Nature Publishing Group UK 2021-11-23 /pmc/articles/PMC8611030/ /pubmed/34815502 http://dx.doi.org/10.1038/s41598-021-02254-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Sabbagh, Quentin André-Grégoire, Gwennan Alves-Nicolau, Carolina Dupont, Aurélien Bidère, Nicolas Jouglar, Emmanuel Guével, Laëtitia Frénel, Jean-Sébastien Gavard, Julie The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients |
title | The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients |
title_full | The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients |
title_fullStr | The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients |
title_full_unstemmed | The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients |
title_short | The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients |
title_sort | von willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611030/ https://www.ncbi.nlm.nih.gov/pubmed/34815502 http://dx.doi.org/10.1038/s41598-021-02254-7 |
work_keys_str_mv | AT sabbaghquentin thevonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT andregregoiregwennan thevonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT alvesnicolaucarolina thevonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT dupontaurelien thevonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT biderenicolas thevonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT jouglaremmanuel thevonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT guevellaetitia thevonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT freneljeansebastien thevonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT gavardjulie thevonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT sabbaghquentin vonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT andregregoiregwennan vonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT alvesnicolaucarolina vonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT dupontaurelien vonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT biderenicolas vonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT jouglaremmanuel vonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT guevellaetitia vonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT freneljeansebastien vonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients AT gavardjulie vonwillebrandfactorstampsplasmaticextracellularvesiclesfromglioblastomapatients |