Epitachophoresis is a novel versatile total nucleic acid extraction method

Epitachophoresis is a novel next generation extraction system capable of isolating DNA and RNA simultaneously from clinically relevant samples. Here we build on the versatility of Epitachophoresis by extracting diverse nucleic acids ranging in lengths (20 nt–290 Kbp). The quality of extracted miRNA,...

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Autores principales: Datinska, Vladimira, Gheibi, Pantea, Jefferson, Keynttisha, Yang, Jaeyoung, Paladugu, Sri, Dallett, Carolina, Voracova, Ivona, Foret, Frantisek, Astier, Yann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611068/
https://www.ncbi.nlm.nih.gov/pubmed/34815497
http://dx.doi.org/10.1038/s41598-021-02214-1
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author Datinska, Vladimira
Gheibi, Pantea
Jefferson, Keynttisha
Yang, Jaeyoung
Paladugu, Sri
Dallett, Carolina
Voracova, Ivona
Foret, Frantisek
Astier, Yann
author_facet Datinska, Vladimira
Gheibi, Pantea
Jefferson, Keynttisha
Yang, Jaeyoung
Paladugu, Sri
Dallett, Carolina
Voracova, Ivona
Foret, Frantisek
Astier, Yann
author_sort Datinska, Vladimira
collection PubMed
description Epitachophoresis is a novel next generation extraction system capable of isolating DNA and RNA simultaneously from clinically relevant samples. Here we build on the versatility of Epitachophoresis by extracting diverse nucleic acids ranging in lengths (20 nt–290 Kbp). The quality of extracted miRNA, mRNA and gDNA was assessed by downstream Next-Generation Sequencing.
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spelling pubmed-86110682021-11-26 Epitachophoresis is a novel versatile total nucleic acid extraction method Datinska, Vladimira Gheibi, Pantea Jefferson, Keynttisha Yang, Jaeyoung Paladugu, Sri Dallett, Carolina Voracova, Ivona Foret, Frantisek Astier, Yann Sci Rep Article Epitachophoresis is a novel next generation extraction system capable of isolating DNA and RNA simultaneously from clinically relevant samples. Here we build on the versatility of Epitachophoresis by extracting diverse nucleic acids ranging in lengths (20 nt–290 Kbp). The quality of extracted miRNA, mRNA and gDNA was assessed by downstream Next-Generation Sequencing. Nature Publishing Group UK 2021-11-23 /pmc/articles/PMC8611068/ /pubmed/34815497 http://dx.doi.org/10.1038/s41598-021-02214-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Datinska, Vladimira
Gheibi, Pantea
Jefferson, Keynttisha
Yang, Jaeyoung
Paladugu, Sri
Dallett, Carolina
Voracova, Ivona
Foret, Frantisek
Astier, Yann
Epitachophoresis is a novel versatile total nucleic acid extraction method
title Epitachophoresis is a novel versatile total nucleic acid extraction method
title_full Epitachophoresis is a novel versatile total nucleic acid extraction method
title_fullStr Epitachophoresis is a novel versatile total nucleic acid extraction method
title_full_unstemmed Epitachophoresis is a novel versatile total nucleic acid extraction method
title_short Epitachophoresis is a novel versatile total nucleic acid extraction method
title_sort epitachophoresis is a novel versatile total nucleic acid extraction method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611068/
https://www.ncbi.nlm.nih.gov/pubmed/34815497
http://dx.doi.org/10.1038/s41598-021-02214-1
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