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Evaluation of SEPT2 and SEPT4 transcript contents in spermatozoa from men with asthenozoospermia and teratozoospermia

BACKGROUND AND AIMS: Motility and morphological defects of spermatozoa can cause male infertility. Sperm RNAs are related to sperm quality. They are considered to have clinical values as a biomarker for assessing sperm quality and fertility potential. The annulus, located in the mammalian sperm tail...

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Autores principales: Mazaheri Moghaddam, Madiheh, Mazaheri Moghaddam, Marziyeh, Amini, Mohammad, Bahramzadeh, Behzad, Baghbanzadeh, Amir, Biglari, Alireza, Sakhinia, Ebrahim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611181/
https://www.ncbi.nlm.nih.gov/pubmed/34849407
http://dx.doi.org/10.1002/hsr2.436
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author Mazaheri Moghaddam, Madiheh
Mazaheri Moghaddam, Marziyeh
Amini, Mohammad
Bahramzadeh, Behzad
Baghbanzadeh, Amir
Biglari, Alireza
Sakhinia, Ebrahim
author_facet Mazaheri Moghaddam, Madiheh
Mazaheri Moghaddam, Marziyeh
Amini, Mohammad
Bahramzadeh, Behzad
Baghbanzadeh, Amir
Biglari, Alireza
Sakhinia, Ebrahim
author_sort Mazaheri Moghaddam, Madiheh
collection PubMed
description BACKGROUND AND AIMS: Motility and morphological defects of spermatozoa can cause male infertility. Sperm RNAs are related to sperm quality. They are considered to have clinical values as a biomarker for assessing sperm quality and fertility potential. The annulus, located in the mammalian sperm tail, is required for motility and terminal differentiation of the spermatozoa. SEPT2, 4, 6, 7, and 12 proteins are the main components of the annulus in the sperm tail. The study aimed to evaluate SEPT2 and SEPT4 mRNA contents in the spermatozoa of patients with asthenozoospermia and teratozoospermia. METHODS: We evaluated transcript levels of SEPT2 and SEPT4 in the sperm samples of 20 asthenozoospermic, 20 teratozoospermic, and 20 normozoospermic samples using quantitative PCR. RESULTS: The SEPT2 transcript level was significantly decreased in the asthenozoospermia samples compared with the normal group (P = .013). However, SEPT4 was not significantly different between these two groups. The transcript levels of SEPT2 and SEPT4 were not statistically different between teratozoospermic and normozoospermic groups. CONCLUSION: In conclusion, downregulation of SEPT2 in patients with asthenozoospermia appears to be associated with poor sperm motility.
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spelling pubmed-86111812021-11-29 Evaluation of SEPT2 and SEPT4 transcript contents in spermatozoa from men with asthenozoospermia and teratozoospermia Mazaheri Moghaddam, Madiheh Mazaheri Moghaddam, Marziyeh Amini, Mohammad Bahramzadeh, Behzad Baghbanzadeh, Amir Biglari, Alireza Sakhinia, Ebrahim Health Sci Rep Research Articles BACKGROUND AND AIMS: Motility and morphological defects of spermatozoa can cause male infertility. Sperm RNAs are related to sperm quality. They are considered to have clinical values as a biomarker for assessing sperm quality and fertility potential. The annulus, located in the mammalian sperm tail, is required for motility and terminal differentiation of the spermatozoa. SEPT2, 4, 6, 7, and 12 proteins are the main components of the annulus in the sperm tail. The study aimed to evaluate SEPT2 and SEPT4 mRNA contents in the spermatozoa of patients with asthenozoospermia and teratozoospermia. METHODS: We evaluated transcript levels of SEPT2 and SEPT4 in the sperm samples of 20 asthenozoospermic, 20 teratozoospermic, and 20 normozoospermic samples using quantitative PCR. RESULTS: The SEPT2 transcript level was significantly decreased in the asthenozoospermia samples compared with the normal group (P = .013). However, SEPT4 was not significantly different between these two groups. The transcript levels of SEPT2 and SEPT4 were not statistically different between teratozoospermic and normozoospermic groups. CONCLUSION: In conclusion, downregulation of SEPT2 in patients with asthenozoospermia appears to be associated with poor sperm motility. John Wiley and Sons Inc. 2021-11-23 /pmc/articles/PMC8611181/ /pubmed/34849407 http://dx.doi.org/10.1002/hsr2.436 Text en © 2021 The Authors. Health Science Reports published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Mazaheri Moghaddam, Madiheh
Mazaheri Moghaddam, Marziyeh
Amini, Mohammad
Bahramzadeh, Behzad
Baghbanzadeh, Amir
Biglari, Alireza
Sakhinia, Ebrahim
Evaluation of SEPT2 and SEPT4 transcript contents in spermatozoa from men with asthenozoospermia and teratozoospermia
title Evaluation of SEPT2 and SEPT4 transcript contents in spermatozoa from men with asthenozoospermia and teratozoospermia
title_full Evaluation of SEPT2 and SEPT4 transcript contents in spermatozoa from men with asthenozoospermia and teratozoospermia
title_fullStr Evaluation of SEPT2 and SEPT4 transcript contents in spermatozoa from men with asthenozoospermia and teratozoospermia
title_full_unstemmed Evaluation of SEPT2 and SEPT4 transcript contents in spermatozoa from men with asthenozoospermia and teratozoospermia
title_short Evaluation of SEPT2 and SEPT4 transcript contents in spermatozoa from men with asthenozoospermia and teratozoospermia
title_sort evaluation of sept2 and sept4 transcript contents in spermatozoa from men with asthenozoospermia and teratozoospermia
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611181/
https://www.ncbi.nlm.nih.gov/pubmed/34849407
http://dx.doi.org/10.1002/hsr2.436
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