Protective effects of coenzyme Q(10) on cell damage induced by hydrogen peroxides in cultured skin fibroblasts

Cellular senescence is an intricate and multifactorial phenomenon, which is characterized by an irreversible cellular growth arrest, it is caused in response to irretrievably DNA damage, telomere shorting, activation of oncogene, and oxidative stress. Human diploid fibroblasts are a well-established experimental model for premature senescence-related studies, and exposure of fibroblasts to H(2)O(2) is widely used as a SIPS model. Recently, it has been reported many studies of CoQ(10) as to anti-aging effects, however the effect of CoQ(10) on H(2)O(2)-induced SIPS model of human skin fibroblasts has not been understood. So that, we investigated that human skin fibroblasts were used to investigate the prevention effect of CoQ(10) against H(2)O(2)-induced SIPS model. We created SIPS model fibroblasts with treatment of 100 μM H(2)O(2) for 2 h. In this study, CoQ(10) also increased cell viability and mRNA levels of type I, IV collagen and protein level of type I collagen. Moreover, it is shown that CoQ(10) suppressed oxidative stress, degradation of collagen by increasing MMP expression, and decreasing senescence-associated phenotypes (e.g. SA-βgal positive staining and SASP) for preventing skin aging via H(2)O(2)-induced SIPS model. These results suggested that CoQ(10) has possibility to be contributory for extension of healthy life expectancy in Japan.