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3D Bioprinting of Biomimetic Bilayered Scaffold Consisting of Decellularized Extracellular Matrix and Silk Fibroin for Osteochondral Repair

Recently, three-dimensional (3D) bioprinting technology is becoming an appealing approach for osteochondral repair. However, it is challenging to develop a bilayered scaffold with anisotropic structural properties to mimic a native osteochondral tissue. Herein, we developed a bioink consisting of de...

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Autores principales: Zhang, Xiao, Liu, Yang, Zuo, Qiang, Wang, Qingyun, Li, Zuxi, Yan, Kai, Yuan, Tao, Zhang, Yi, Shen, Kai, Xie, Rui, Fan, Weimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Whioce Publishing Pte. Ltd. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611412/
https://www.ncbi.nlm.nih.gov/pubmed/34825099
http://dx.doi.org/10.18063/ijb.v7i4.401
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author Zhang, Xiao
Liu, Yang
Zuo, Qiang
Wang, Qingyun
Li, Zuxi
Yan, Kai
Yuan, Tao
Zhang, Yi
Shen, Kai
Xie, Rui
Fan, Weimin
author_facet Zhang, Xiao
Liu, Yang
Zuo, Qiang
Wang, Qingyun
Li, Zuxi
Yan, Kai
Yuan, Tao
Zhang, Yi
Shen, Kai
Xie, Rui
Fan, Weimin
author_sort Zhang, Xiao
collection PubMed
description Recently, three-dimensional (3D) bioprinting technology is becoming an appealing approach for osteochondral repair. However, it is challenging to develop a bilayered scaffold with anisotropic structural properties to mimic a native osteochondral tissue. Herein, we developed a bioink consisting of decellularized extracellular matrix and silk fibroin to print the bilayered scaffold. The bilayered scaffold mimics the natural osteochondral tissue by controlling the composition, mechanical properties, and growth factor release in each layer of the scaffold. The in vitro results show that each layer of scaffolds had a suitable mechanical strength and degradation rate. Furthermore, the scaffolds encapsulating transforming growth factor-beta (TGF-β) and bone morphogenetic protein-2 (BMP-2) can act as a controlled release system and promote directed differentiation of bone marrow-derived mesenchymal stem cells. Furthermore, the in vivo experiments suggested that the scaffolds loaded with growth factors promoted osteochondral regeneration in the rabbit knee joint model. Consequently, the biomimetic bilayered scaffold loaded with TGF-β and BMP-2 would be a promising strategy for osteochondral repair.
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spelling pubmed-86114122021-11-24 3D Bioprinting of Biomimetic Bilayered Scaffold Consisting of Decellularized Extracellular Matrix and Silk Fibroin for Osteochondral Repair Zhang, Xiao Liu, Yang Zuo, Qiang Wang, Qingyun Li, Zuxi Yan, Kai Yuan, Tao Zhang, Yi Shen, Kai Xie, Rui Fan, Weimin Int J Bioprint Research Article Recently, three-dimensional (3D) bioprinting technology is becoming an appealing approach for osteochondral repair. However, it is challenging to develop a bilayered scaffold with anisotropic structural properties to mimic a native osteochondral tissue. Herein, we developed a bioink consisting of decellularized extracellular matrix and silk fibroin to print the bilayered scaffold. The bilayered scaffold mimics the natural osteochondral tissue by controlling the composition, mechanical properties, and growth factor release in each layer of the scaffold. The in vitro results show that each layer of scaffolds had a suitable mechanical strength and degradation rate. Furthermore, the scaffolds encapsulating transforming growth factor-beta (TGF-β) and bone morphogenetic protein-2 (BMP-2) can act as a controlled release system and promote directed differentiation of bone marrow-derived mesenchymal stem cells. Furthermore, the in vivo experiments suggested that the scaffolds loaded with growth factors promoted osteochondral regeneration in the rabbit knee joint model. Consequently, the biomimetic bilayered scaffold loaded with TGF-β and BMP-2 would be a promising strategy for osteochondral repair. Whioce Publishing Pte. Ltd. 2021-09-14 /pmc/articles/PMC8611412/ /pubmed/34825099 http://dx.doi.org/10.18063/ijb.v7i4.401 Text en Copyright: © 2021 Zhang, et al. https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Attribution-NonCommercial 4.0 International 4.0 (CC BY-NC 4.0), which permits all non-commercial use, distribution, and reproduction in any medium provided the original work is properly cited.
spellingShingle Research Article
Zhang, Xiao
Liu, Yang
Zuo, Qiang
Wang, Qingyun
Li, Zuxi
Yan, Kai
Yuan, Tao
Zhang, Yi
Shen, Kai
Xie, Rui
Fan, Weimin
3D Bioprinting of Biomimetic Bilayered Scaffold Consisting of Decellularized Extracellular Matrix and Silk Fibroin for Osteochondral Repair
title 3D Bioprinting of Biomimetic Bilayered Scaffold Consisting of Decellularized Extracellular Matrix and Silk Fibroin for Osteochondral Repair
title_full 3D Bioprinting of Biomimetic Bilayered Scaffold Consisting of Decellularized Extracellular Matrix and Silk Fibroin for Osteochondral Repair
title_fullStr 3D Bioprinting of Biomimetic Bilayered Scaffold Consisting of Decellularized Extracellular Matrix and Silk Fibroin for Osteochondral Repair
title_full_unstemmed 3D Bioprinting of Biomimetic Bilayered Scaffold Consisting of Decellularized Extracellular Matrix and Silk Fibroin for Osteochondral Repair
title_short 3D Bioprinting of Biomimetic Bilayered Scaffold Consisting of Decellularized Extracellular Matrix and Silk Fibroin for Osteochondral Repair
title_sort 3d bioprinting of biomimetic bilayered scaffold consisting of decellularized extracellular matrix and silk fibroin for osteochondral repair
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611412/
https://www.ncbi.nlm.nih.gov/pubmed/34825099
http://dx.doi.org/10.18063/ijb.v7i4.401
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