Production and use of encapsidated RNA mimics as positive control reagents for SARS-CoV-2 RT-qPCR diagnostics

The current gold standard technique for SARS-CoV-2 diagnostics is hydrolysis probe-based RT-qPCR. Reliable testing requires reliable control reagents to monitor the efficiency of RNA extraction, reverse transcription and PCR amplification. Here we describe a custom RNA packaging system from the plan...

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Detalles Bibliográficos
Autores principales: Peyret, Hadrien, Groppelli, Elisabetta, Clark, David, Eckersley, Nicholas, Planche, Tim, Ma, Julian, Lomonossoff, George P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier/North-Holland Biomedical Press 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611828/
https://www.ncbi.nlm.nih.gov/pubmed/34838537
http://dx.doi.org/10.1016/j.jviromet.2021.114372

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