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Revealing RCOR2 as a regulatory component of nuclear speckles

BACKGROUND: Nuclear processes such as transcription and RNA maturation can be impacted by subnuclear compartmentalization in condensates and nuclear bodies. Here, we characterize the nature of nuclear granules formed by REST corepressor 2 (RCOR2), a nuclear protein essential for pluripotency mainten...

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Autores principales: Rivera, Carlos, Verbel-Vergara, Daniel, Arancibia, Duxan, Lappala, Anna, González, Marcela, Guzmán, Fabián, Merello, Gianluca, Lee, Jeannie T., Andrés, María Estela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611983/
https://www.ncbi.nlm.nih.gov/pubmed/34819154
http://dx.doi.org/10.1186/s13072-021-00425-4
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author Rivera, Carlos
Verbel-Vergara, Daniel
Arancibia, Duxan
Lappala, Anna
González, Marcela
Guzmán, Fabián
Merello, Gianluca
Lee, Jeannie T.
Andrés, María Estela
author_facet Rivera, Carlos
Verbel-Vergara, Daniel
Arancibia, Duxan
Lappala, Anna
González, Marcela
Guzmán, Fabián
Merello, Gianluca
Lee, Jeannie T.
Andrés, María Estela
author_sort Rivera, Carlos
collection PubMed
description BACKGROUND: Nuclear processes such as transcription and RNA maturation can be impacted by subnuclear compartmentalization in condensates and nuclear bodies. Here, we characterize the nature of nuclear granules formed by REST corepressor 2 (RCOR2), a nuclear protein essential for pluripotency maintenance and central nervous system development. RESULTS: Using biochemical approaches and high-resolution microscopy, we reveal that RCOR2 is localized in nuclear speckles across multiple cell types, including neurons in the brain. RCOR2 forms complexes with nuclear speckle components such as SON, SRSF7, and SRRM2. When cells are exposed to chemical stress, RCOR2 behaves as a core component of the nuclear speckle and is stabilized by RNA. In turn, nuclear speckle morphology appears to depend on RCOR2. Specifically, RCOR2 knockdown results larger nuclear speckles, whereas overexpressing RCOR2 leads to smaller and rounder nuclear speckles. CONCLUSION: Our study suggests that RCOR2 is a regulatory component of the nuclear speckle bodies, setting this co-repressor protein as a factor that controls nuclear speckles behavior. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13072-021-00425-4.
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spelling pubmed-86119832021-11-29 Revealing RCOR2 as a regulatory component of nuclear speckles Rivera, Carlos Verbel-Vergara, Daniel Arancibia, Duxan Lappala, Anna González, Marcela Guzmán, Fabián Merello, Gianluca Lee, Jeannie T. Andrés, María Estela Epigenetics Chromatin Research BACKGROUND: Nuclear processes such as transcription and RNA maturation can be impacted by subnuclear compartmentalization in condensates and nuclear bodies. Here, we characterize the nature of nuclear granules formed by REST corepressor 2 (RCOR2), a nuclear protein essential for pluripotency maintenance and central nervous system development. RESULTS: Using biochemical approaches and high-resolution microscopy, we reveal that RCOR2 is localized in nuclear speckles across multiple cell types, including neurons in the brain. RCOR2 forms complexes with nuclear speckle components such as SON, SRSF7, and SRRM2. When cells are exposed to chemical stress, RCOR2 behaves as a core component of the nuclear speckle and is stabilized by RNA. In turn, nuclear speckle morphology appears to depend on RCOR2. Specifically, RCOR2 knockdown results larger nuclear speckles, whereas overexpressing RCOR2 leads to smaller and rounder nuclear speckles. CONCLUSION: Our study suggests that RCOR2 is a regulatory component of the nuclear speckle bodies, setting this co-repressor protein as a factor that controls nuclear speckles behavior. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13072-021-00425-4. BioMed Central 2021-11-24 /pmc/articles/PMC8611983/ /pubmed/34819154 http://dx.doi.org/10.1186/s13072-021-00425-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Rivera, Carlos
Verbel-Vergara, Daniel
Arancibia, Duxan
Lappala, Anna
González, Marcela
Guzmán, Fabián
Merello, Gianluca
Lee, Jeannie T.
Andrés, María Estela
Revealing RCOR2 as a regulatory component of nuclear speckles
title Revealing RCOR2 as a regulatory component of nuclear speckles
title_full Revealing RCOR2 as a regulatory component of nuclear speckles
title_fullStr Revealing RCOR2 as a regulatory component of nuclear speckles
title_full_unstemmed Revealing RCOR2 as a regulatory component of nuclear speckles
title_short Revealing RCOR2 as a regulatory component of nuclear speckles
title_sort revealing rcor2 as a regulatory component of nuclear speckles
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8611983/
https://www.ncbi.nlm.nih.gov/pubmed/34819154
http://dx.doi.org/10.1186/s13072-021-00425-4
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