Cargando…
FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot
To survive, cells must constantly resist mechanical stress. In plants, this involves the reinforcement of cell walls, notably through microtubule-dependent cellulose deposition. How wall sensing might contribute to this response is unknown. Here, we tested whether the microtubule response to stress...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8612563/ https://www.ncbi.nlm.nih.gov/pubmed/34767544 http://dx.doi.org/10.1371/journal.pbio.3001454 |
_version_ | 1784603473903353856 |
---|---|
author | Malivert, Alice Erguvan, Özer Chevallier, Antoine Dehem, Antoine Friaud, Rodrigue Liu, Mengying Martin, Marjolaine Peyraud, Théophile Hamant, Olivier Verger, Stéphane |
author_facet | Malivert, Alice Erguvan, Özer Chevallier, Antoine Dehem, Antoine Friaud, Rodrigue Liu, Mengying Martin, Marjolaine Peyraud, Théophile Hamant, Olivier Verger, Stéphane |
author_sort | Malivert, Alice |
collection | PubMed |
description | To survive, cells must constantly resist mechanical stress. In plants, this involves the reinforcement of cell walls, notably through microtubule-dependent cellulose deposition. How wall sensing might contribute to this response is unknown. Here, we tested whether the microtubule response to stress acts downstream of known wall sensors. Using a multistep screen with 11 mutant lines, we identify FERONIA (FER) as the primary candidate for the cell’s response to stress in the shoot. However, this does not imply that FER acts upstream of the microtubule response to stress. In fact, when performing mechanical perturbations, we instead show that the expected microtubule response to stress does not require FER. We reveal that the feronia phenotype can be partially rescued by reducing tensile stress levels. Conversely, in the absence of both microtubules and FER, cells appear to swell and burst. Altogether, this shows that the microtubule response to stress acts as an independent pathway to resist stress, in parallel to FER. We propose that both pathways are required to maintain the mechanical integrity of plant cells. |
format | Online Article Text |
id | pubmed-8612563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-86125632021-11-25 FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot Malivert, Alice Erguvan, Özer Chevallier, Antoine Dehem, Antoine Friaud, Rodrigue Liu, Mengying Martin, Marjolaine Peyraud, Théophile Hamant, Olivier Verger, Stéphane PLoS Biol Research Article To survive, cells must constantly resist mechanical stress. In plants, this involves the reinforcement of cell walls, notably through microtubule-dependent cellulose deposition. How wall sensing might contribute to this response is unknown. Here, we tested whether the microtubule response to stress acts downstream of known wall sensors. Using a multistep screen with 11 mutant lines, we identify FERONIA (FER) as the primary candidate for the cell’s response to stress in the shoot. However, this does not imply that FER acts upstream of the microtubule response to stress. In fact, when performing mechanical perturbations, we instead show that the expected microtubule response to stress does not require FER. We reveal that the feronia phenotype can be partially rescued by reducing tensile stress levels. Conversely, in the absence of both microtubules and FER, cells appear to swell and burst. Altogether, this shows that the microtubule response to stress acts as an independent pathway to resist stress, in parallel to FER. We propose that both pathways are required to maintain the mechanical integrity of plant cells. Public Library of Science 2021-11-12 /pmc/articles/PMC8612563/ /pubmed/34767544 http://dx.doi.org/10.1371/journal.pbio.3001454 Text en © 2021 Malivert et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Malivert, Alice Erguvan, Özer Chevallier, Antoine Dehem, Antoine Friaud, Rodrigue Liu, Mengying Martin, Marjolaine Peyraud, Théophile Hamant, Olivier Verger, Stéphane FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot |
title | FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot |
title_full | FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot |
title_fullStr | FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot |
title_full_unstemmed | FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot |
title_short | FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot |
title_sort | feronia and microtubules independently contribute to mechanical integrity in the arabidopsis shoot |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8612563/ https://www.ncbi.nlm.nih.gov/pubmed/34767544 http://dx.doi.org/10.1371/journal.pbio.3001454 |
work_keys_str_mv | AT malivertalice feroniaandmicrotubulesindependentlycontributetomechanicalintegrityinthearabidopsisshoot AT erguvanozer feroniaandmicrotubulesindependentlycontributetomechanicalintegrityinthearabidopsisshoot AT chevallierantoine feroniaandmicrotubulesindependentlycontributetomechanicalintegrityinthearabidopsisshoot AT dehemantoine feroniaandmicrotubulesindependentlycontributetomechanicalintegrityinthearabidopsisshoot AT friaudrodrigue feroniaandmicrotubulesindependentlycontributetomechanicalintegrityinthearabidopsisshoot AT liumengying feroniaandmicrotubulesindependentlycontributetomechanicalintegrityinthearabidopsisshoot AT martinmarjolaine feroniaandmicrotubulesindependentlycontributetomechanicalintegrityinthearabidopsisshoot AT peyraudtheophile feroniaandmicrotubulesindependentlycontributetomechanicalintegrityinthearabidopsisshoot AT hamantolivier feroniaandmicrotubulesindependentlycontributetomechanicalintegrityinthearabidopsisshoot AT vergerstephane feroniaandmicrotubulesindependentlycontributetomechanicalintegrityinthearabidopsisshoot |