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Distinct dendritic Ca(2+) spike forms produce opposing input-output transformations in rat CA3 pyramidal cells

Proper integration of different inputs targeting the dendritic tree of CA3 pyramidal cells (CA3PCs) is critical for associative learning and recall. Dendritic Ca(2+) spikes have been proposed to perform associative computations in other PC types by detecting conjunctive activation of different affer...

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Autores principales: Magó, Ádám, Kis, Noémi, Lükő, Balázs, Makara, Judit K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8612760/
https://www.ncbi.nlm.nih.gov/pubmed/34817378
http://dx.doi.org/10.7554/eLife.74493
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author Magó, Ádám
Kis, Noémi
Lükő, Balázs
Makara, Judit K
author_facet Magó, Ádám
Kis, Noémi
Lükő, Balázs
Makara, Judit K
author_sort Magó, Ádám
collection PubMed
description Proper integration of different inputs targeting the dendritic tree of CA3 pyramidal cells (CA3PCs) is critical for associative learning and recall. Dendritic Ca(2+) spikes have been proposed to perform associative computations in other PC types by detecting conjunctive activation of different afferent input pathways, initiating afterdepolarization (ADP), and triggering burst firing. Implementation of such operations fundamentally depends on the actual biophysical properties of dendritic Ca(2+) spikes; yet little is known about these properties in dendrites of CA3PCs. Using dendritic patch-clamp recordings and two-photon Ca(2+) imaging in acute slices from male rats, we report that, unlike CA1PCs, distal apical trunk dendrites of CA3PCs exhibit distinct forms of dendritic Ca(2+) spikes. Besides ADP-type global Ca(2+) spikes, a majority of dendrites expresses a novel, fast Ca(2+) spike type that is initiated locally without bAPs, can recruit additional Na(+) currents, and is compartmentalized to the activated dendritic subtree. Occurrence of the different Ca(2+) spike types correlates with dendritic structure, indicating morpho-functional heterogeneity among CA3PCs. Importantly, ADPs and dendritically initiated spikes produce opposing somatic output: bursts versus strictly single-action potentials, respectively. The uncovered variability of dendritic Ca(2+) spikes may underlie heterogeneous input-output transformation and bursting properties of CA3PCs, and might specifically contribute to key associative and non-associative computations performed by the CA3 network.
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spelling pubmed-86127602021-11-26 Distinct dendritic Ca(2+) spike forms produce opposing input-output transformations in rat CA3 pyramidal cells Magó, Ádám Kis, Noémi Lükő, Balázs Makara, Judit K eLife Neuroscience Proper integration of different inputs targeting the dendritic tree of CA3 pyramidal cells (CA3PCs) is critical for associative learning and recall. Dendritic Ca(2+) spikes have been proposed to perform associative computations in other PC types by detecting conjunctive activation of different afferent input pathways, initiating afterdepolarization (ADP), and triggering burst firing. Implementation of such operations fundamentally depends on the actual biophysical properties of dendritic Ca(2+) spikes; yet little is known about these properties in dendrites of CA3PCs. Using dendritic patch-clamp recordings and two-photon Ca(2+) imaging in acute slices from male rats, we report that, unlike CA1PCs, distal apical trunk dendrites of CA3PCs exhibit distinct forms of dendritic Ca(2+) spikes. Besides ADP-type global Ca(2+) spikes, a majority of dendrites expresses a novel, fast Ca(2+) spike type that is initiated locally without bAPs, can recruit additional Na(+) currents, and is compartmentalized to the activated dendritic subtree. Occurrence of the different Ca(2+) spike types correlates with dendritic structure, indicating morpho-functional heterogeneity among CA3PCs. Importantly, ADPs and dendritically initiated spikes produce opposing somatic output: bursts versus strictly single-action potentials, respectively. The uncovered variability of dendritic Ca(2+) spikes may underlie heterogeneous input-output transformation and bursting properties of CA3PCs, and might specifically contribute to key associative and non-associative computations performed by the CA3 network. eLife Sciences Publications, Ltd 2021-11-24 /pmc/articles/PMC8612760/ /pubmed/34817378 http://dx.doi.org/10.7554/eLife.74493 Text en © 2021, Magó et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Magó, Ádám
Kis, Noémi
Lükő, Balázs
Makara, Judit K
Distinct dendritic Ca(2+) spike forms produce opposing input-output transformations in rat CA3 pyramidal cells
title Distinct dendritic Ca(2+) spike forms produce opposing input-output transformations in rat CA3 pyramidal cells
title_full Distinct dendritic Ca(2+) spike forms produce opposing input-output transformations in rat CA3 pyramidal cells
title_fullStr Distinct dendritic Ca(2+) spike forms produce opposing input-output transformations in rat CA3 pyramidal cells
title_full_unstemmed Distinct dendritic Ca(2+) spike forms produce opposing input-output transformations in rat CA3 pyramidal cells
title_short Distinct dendritic Ca(2+) spike forms produce opposing input-output transformations in rat CA3 pyramidal cells
title_sort distinct dendritic ca(2+) spike forms produce opposing input-output transformations in rat ca3 pyramidal cells
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8612760/
https://www.ncbi.nlm.nih.gov/pubmed/34817378
http://dx.doi.org/10.7554/eLife.74493
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