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First molecular description of Macracanthorhynchus hirudinaceus in wild boars from Italy with pathomorphological and epidemiological insights

Macracanthorhynchus hirudinaceus is a zoonotic parasite affecting suids worldwide which are the definitive hosts for this helminth species. Macracanthorhynchus hirudinaceus is of significant economic and management concern due to its pathogenicity, causing intestinal obstruction and perforation in t...

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Autores principales: Dessì, Giorgia, Cabras, Pierangela, Mehmood, Naunain, Ahmed, Fahad, Porcu, Francesca, Veneziano, Vincenzo, Burrai, Giovanni Pietro, Tamponi, Claudia, Scala, Antonio, Varcasia, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8612822/
https://www.ncbi.nlm.nih.gov/pubmed/34820718
http://dx.doi.org/10.1007/s00436-021-07386-3
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author Dessì, Giorgia
Cabras, Pierangela
Mehmood, Naunain
Ahmed, Fahad
Porcu, Francesca
Veneziano, Vincenzo
Burrai, Giovanni Pietro
Tamponi, Claudia
Scala, Antonio
Varcasia, Antonio
author_facet Dessì, Giorgia
Cabras, Pierangela
Mehmood, Naunain
Ahmed, Fahad
Porcu, Francesca
Veneziano, Vincenzo
Burrai, Giovanni Pietro
Tamponi, Claudia
Scala, Antonio
Varcasia, Antonio
author_sort Dessì, Giorgia
collection PubMed
description Macracanthorhynchus hirudinaceus is a zoonotic parasite affecting suids worldwide which are the definitive hosts for this helminth species. Macracanthorhynchus hirudinaceus is of significant economic and management concern due to its pathogenicity, causing intestinal obstruction and perforation in the definitive hosts. Current study is the preliminary investigation from Sardinia, Italy, reporting the pathomorphological findings and molecular characterization of M. hirudinaceus in the wild boars (Sus scrofa meridionalis). A total of 59 wild boars were examined showing acanthocephalan infection in 8 (13.6%) animals. In total, 49 parasites were collected with a mean intensity of 6.1. Comparatively higher infection levels were observed for males (16.7%) and young boars (14.3%); however, these epidemiological differences were statistically non-significant. Histopathological examination revealed the presence of a variable number of nodules (∼5 mm) in the intestine of M. hirudinaceus infested animals surrounded by a hyperemic-hemorrhagic halo. Several parasites were recovered from the intestinal lumen attached by the means of characteristic hooks showing necrosis in muscle layers. A moderate number of plump reactive fibroblasts and lesser numbers of fibrocytes were embedded with and at the borders of the inflammatory nodules in a moderate amount of homogeneous intensely eosinophilic fibrillary material rupturing the cell membrane. For molecular characterization, six isolated worms were amplified for the partial mitochondrial cox1 gene showing distinct interindividual variations. This first pathological and molecular description from southern Europe provided new knowledge about the diffusion of M. hirudinaceus in wild boars, furthering the research into the origin and transmission status of M. hirudinaceus in endemic localities.
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spelling pubmed-86128222021-11-26 First molecular description of Macracanthorhynchus hirudinaceus in wild boars from Italy with pathomorphological and epidemiological insights Dessì, Giorgia Cabras, Pierangela Mehmood, Naunain Ahmed, Fahad Porcu, Francesca Veneziano, Vincenzo Burrai, Giovanni Pietro Tamponi, Claudia Scala, Antonio Varcasia, Antonio Parasitol Res Helminthology - Original Paper Macracanthorhynchus hirudinaceus is a zoonotic parasite affecting suids worldwide which are the definitive hosts for this helminth species. Macracanthorhynchus hirudinaceus is of significant economic and management concern due to its pathogenicity, causing intestinal obstruction and perforation in the definitive hosts. Current study is the preliminary investigation from Sardinia, Italy, reporting the pathomorphological findings and molecular characterization of M. hirudinaceus in the wild boars (Sus scrofa meridionalis). A total of 59 wild boars were examined showing acanthocephalan infection in 8 (13.6%) animals. In total, 49 parasites were collected with a mean intensity of 6.1. Comparatively higher infection levels were observed for males (16.7%) and young boars (14.3%); however, these epidemiological differences were statistically non-significant. Histopathological examination revealed the presence of a variable number of nodules (∼5 mm) in the intestine of M. hirudinaceus infested animals surrounded by a hyperemic-hemorrhagic halo. Several parasites were recovered from the intestinal lumen attached by the means of characteristic hooks showing necrosis in muscle layers. A moderate number of plump reactive fibroblasts and lesser numbers of fibrocytes were embedded with and at the borders of the inflammatory nodules in a moderate amount of homogeneous intensely eosinophilic fibrillary material rupturing the cell membrane. For molecular characterization, six isolated worms were amplified for the partial mitochondrial cox1 gene showing distinct interindividual variations. This first pathological and molecular description from southern Europe provided new knowledge about the diffusion of M. hirudinaceus in wild boars, furthering the research into the origin and transmission status of M. hirudinaceus in endemic localities. Springer Berlin Heidelberg 2021-11-25 2022 /pmc/articles/PMC8612822/ /pubmed/34820718 http://dx.doi.org/10.1007/s00436-021-07386-3 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Helminthology - Original Paper
Dessì, Giorgia
Cabras, Pierangela
Mehmood, Naunain
Ahmed, Fahad
Porcu, Francesca
Veneziano, Vincenzo
Burrai, Giovanni Pietro
Tamponi, Claudia
Scala, Antonio
Varcasia, Antonio
First molecular description of Macracanthorhynchus hirudinaceus in wild boars from Italy with pathomorphological and epidemiological insights
title First molecular description of Macracanthorhynchus hirudinaceus in wild boars from Italy with pathomorphological and epidemiological insights
title_full First molecular description of Macracanthorhynchus hirudinaceus in wild boars from Italy with pathomorphological and epidemiological insights
title_fullStr First molecular description of Macracanthorhynchus hirudinaceus in wild boars from Italy with pathomorphological and epidemiological insights
title_full_unstemmed First molecular description of Macracanthorhynchus hirudinaceus in wild boars from Italy with pathomorphological and epidemiological insights
title_short First molecular description of Macracanthorhynchus hirudinaceus in wild boars from Italy with pathomorphological and epidemiological insights
title_sort first molecular description of macracanthorhynchus hirudinaceus in wild boars from italy with pathomorphological and epidemiological insights
topic Helminthology - Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8612822/
https://www.ncbi.nlm.nih.gov/pubmed/34820718
http://dx.doi.org/10.1007/s00436-021-07386-3
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