Cargando…

Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections

BACKGROUND: Serologic cross-reactivity between hantaviruses often complicates the interpretation of the results. AIM: To analyze the diagnostic value of indirect immunofluorescence assay (IFA) and western blot (WB) in the diagnosis of hantavirus infections. METHODS: One hundred eighty-eight serum sa...

Descripción completa

Detalles Bibliográficos
Autores principales: Vilibic-Cavlek, Tatjana, Barbic, Ljubo, Stevanovic, Vladimir, Savic, Vladimir, Mrzljak, Anna, Bogdanic, Maja, Tabain, Irena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8613714/
https://www.ncbi.nlm.nih.gov/pubmed/34888182
http://dx.doi.org/10.5662/wjm.v11.i6.294
_version_ 1784603693214072832
author Vilibic-Cavlek, Tatjana
Barbic, Ljubo
Stevanovic, Vladimir
Savic, Vladimir
Mrzljak, Anna
Bogdanic, Maja
Tabain, Irena
author_facet Vilibic-Cavlek, Tatjana
Barbic, Ljubo
Stevanovic, Vladimir
Savic, Vladimir
Mrzljak, Anna
Bogdanic, Maja
Tabain, Irena
author_sort Vilibic-Cavlek, Tatjana
collection PubMed
description BACKGROUND: Serologic cross-reactivity between hantaviruses often complicates the interpretation of the results. AIM: To analyze the diagnostic value of indirect immunofluorescence assay (IFA) and western blot (WB) in the diagnosis of hantavirus infections. METHODS: One hundred eighty-eight serum samples from Puumala (PUUV) and Dobrava (DOBV) orthohantavirus infected patients were analyzed. Serology was performed using commercial tests (Euroimmun, Lübeck, Germany). RESULTS: Using IFA, 49.5% of acute-phase samples showed a monotypic response to PUUV, while 50.5% cross-reacted with other hantaviruses. The overall cross-reactivity was higher for immunoglobulin G (IgG) (50.0%) than for immunoglobulin M (IgM) (25.5%). PUUV IgM/IgG antibodies showed low/moderate reactivity with orthohantaviruses Hantaan (12.3%/31.5%), Seoul (7.5%/17.8%), DOBV (5.4%/ 28.1%), and Saaremaa (4.8%/15.7%). Both DOBV IgM and IgG antibodies were broadly reactive with Hantaan (76.2%/95.2%), Saaremaa (80.9%/83.3%), and Seoul (78.6%/85.7%) and moderate with PUUV (28.5%/38.1%). Using a WB, serotyping was successful in most cross-reactive samples (89.5%). CONCLUSION: The presented results indicate that WB is more specific than IFA in the diagnosis of hantavirus infections, confirming serotype in most IFA cross-reactive samples.
format Online
Article
Text
id pubmed-8613714
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Baishideng Publishing Group Inc
record_format MEDLINE/PubMed
spelling pubmed-86137142021-12-08 Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections Vilibic-Cavlek, Tatjana Barbic, Ljubo Stevanovic, Vladimir Savic, Vladimir Mrzljak, Anna Bogdanic, Maja Tabain, Irena World J Methodol Retrospective Study BACKGROUND: Serologic cross-reactivity between hantaviruses often complicates the interpretation of the results. AIM: To analyze the diagnostic value of indirect immunofluorescence assay (IFA) and western blot (WB) in the diagnosis of hantavirus infections. METHODS: One hundred eighty-eight serum samples from Puumala (PUUV) and Dobrava (DOBV) orthohantavirus infected patients were analyzed. Serology was performed using commercial tests (Euroimmun, Lübeck, Germany). RESULTS: Using IFA, 49.5% of acute-phase samples showed a monotypic response to PUUV, while 50.5% cross-reacted with other hantaviruses. The overall cross-reactivity was higher for immunoglobulin G (IgG) (50.0%) than for immunoglobulin M (IgM) (25.5%). PUUV IgM/IgG antibodies showed low/moderate reactivity with orthohantaviruses Hantaan (12.3%/31.5%), Seoul (7.5%/17.8%), DOBV (5.4%/ 28.1%), and Saaremaa (4.8%/15.7%). Both DOBV IgM and IgG antibodies were broadly reactive with Hantaan (76.2%/95.2%), Saaremaa (80.9%/83.3%), and Seoul (78.6%/85.7%) and moderate with PUUV (28.5%/38.1%). Using a WB, serotyping was successful in most cross-reactive samples (89.5%). CONCLUSION: The presented results indicate that WB is more specific than IFA in the diagnosis of hantavirus infections, confirming serotype in most IFA cross-reactive samples. Baishideng Publishing Group Inc 2021-11-20 /pmc/articles/PMC8613714/ /pubmed/34888182 http://dx.doi.org/10.5662/wjm.v11.i6.294 Text en ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved. https://creativecommons.org/licenses/by-nc/4.0/This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
spellingShingle Retrospective Study
Vilibic-Cavlek, Tatjana
Barbic, Ljubo
Stevanovic, Vladimir
Savic, Vladimir
Mrzljak, Anna
Bogdanic, Maja
Tabain, Irena
Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections
title Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections
title_full Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections
title_fullStr Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections
title_full_unstemmed Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections
title_short Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections
title_sort comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections
topic Retrospective Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8613714/
https://www.ncbi.nlm.nih.gov/pubmed/34888182
http://dx.doi.org/10.5662/wjm.v11.i6.294
work_keys_str_mv AT vilibiccavlektatjana comparisonofindirectimmunofluorescenceandwesternblotmethodinthediagnosisofhantavirusinfections
AT barbicljubo comparisonofindirectimmunofluorescenceandwesternblotmethodinthediagnosisofhantavirusinfections
AT stevanovicvladimir comparisonofindirectimmunofluorescenceandwesternblotmethodinthediagnosisofhantavirusinfections
AT savicvladimir comparisonofindirectimmunofluorescenceandwesternblotmethodinthediagnosisofhantavirusinfections
AT mrzljakanna comparisonofindirectimmunofluorescenceandwesternblotmethodinthediagnosisofhantavirusinfections
AT bogdanicmaja comparisonofindirectimmunofluorescenceandwesternblotmethodinthediagnosisofhantavirusinfections
AT tabainirena comparisonofindirectimmunofluorescenceandwesternblotmethodinthediagnosisofhantavirusinfections