Cargando…
Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections
BACKGROUND: Serologic cross-reactivity between hantaviruses often complicates the interpretation of the results. AIM: To analyze the diagnostic value of indirect immunofluorescence assay (IFA) and western blot (WB) in the diagnosis of hantavirus infections. METHODS: One hundred eighty-eight serum sa...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Baishideng Publishing Group Inc
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8613714/ https://www.ncbi.nlm.nih.gov/pubmed/34888182 http://dx.doi.org/10.5662/wjm.v11.i6.294 |
_version_ | 1784603693214072832 |
---|---|
author | Vilibic-Cavlek, Tatjana Barbic, Ljubo Stevanovic, Vladimir Savic, Vladimir Mrzljak, Anna Bogdanic, Maja Tabain, Irena |
author_facet | Vilibic-Cavlek, Tatjana Barbic, Ljubo Stevanovic, Vladimir Savic, Vladimir Mrzljak, Anna Bogdanic, Maja Tabain, Irena |
author_sort | Vilibic-Cavlek, Tatjana |
collection | PubMed |
description | BACKGROUND: Serologic cross-reactivity between hantaviruses often complicates the interpretation of the results. AIM: To analyze the diagnostic value of indirect immunofluorescence assay (IFA) and western blot (WB) in the diagnosis of hantavirus infections. METHODS: One hundred eighty-eight serum samples from Puumala (PUUV) and Dobrava (DOBV) orthohantavirus infected patients were analyzed. Serology was performed using commercial tests (Euroimmun, Lübeck, Germany). RESULTS: Using IFA, 49.5% of acute-phase samples showed a monotypic response to PUUV, while 50.5% cross-reacted with other hantaviruses. The overall cross-reactivity was higher for immunoglobulin G (IgG) (50.0%) than for immunoglobulin M (IgM) (25.5%). PUUV IgM/IgG antibodies showed low/moderate reactivity with orthohantaviruses Hantaan (12.3%/31.5%), Seoul (7.5%/17.8%), DOBV (5.4%/ 28.1%), and Saaremaa (4.8%/15.7%). Both DOBV IgM and IgG antibodies were broadly reactive with Hantaan (76.2%/95.2%), Saaremaa (80.9%/83.3%), and Seoul (78.6%/85.7%) and moderate with PUUV (28.5%/38.1%). Using a WB, serotyping was successful in most cross-reactive samples (89.5%). CONCLUSION: The presented results indicate that WB is more specific than IFA in the diagnosis of hantavirus infections, confirming serotype in most IFA cross-reactive samples. |
format | Online Article Text |
id | pubmed-8613714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Baishideng Publishing Group Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-86137142021-12-08 Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections Vilibic-Cavlek, Tatjana Barbic, Ljubo Stevanovic, Vladimir Savic, Vladimir Mrzljak, Anna Bogdanic, Maja Tabain, Irena World J Methodol Retrospective Study BACKGROUND: Serologic cross-reactivity between hantaviruses often complicates the interpretation of the results. AIM: To analyze the diagnostic value of indirect immunofluorescence assay (IFA) and western blot (WB) in the diagnosis of hantavirus infections. METHODS: One hundred eighty-eight serum samples from Puumala (PUUV) and Dobrava (DOBV) orthohantavirus infected patients were analyzed. Serology was performed using commercial tests (Euroimmun, Lübeck, Germany). RESULTS: Using IFA, 49.5% of acute-phase samples showed a monotypic response to PUUV, while 50.5% cross-reacted with other hantaviruses. The overall cross-reactivity was higher for immunoglobulin G (IgG) (50.0%) than for immunoglobulin M (IgM) (25.5%). PUUV IgM/IgG antibodies showed low/moderate reactivity with orthohantaviruses Hantaan (12.3%/31.5%), Seoul (7.5%/17.8%), DOBV (5.4%/ 28.1%), and Saaremaa (4.8%/15.7%). Both DOBV IgM and IgG antibodies were broadly reactive with Hantaan (76.2%/95.2%), Saaremaa (80.9%/83.3%), and Seoul (78.6%/85.7%) and moderate with PUUV (28.5%/38.1%). Using a WB, serotyping was successful in most cross-reactive samples (89.5%). CONCLUSION: The presented results indicate that WB is more specific than IFA in the diagnosis of hantavirus infections, confirming serotype in most IFA cross-reactive samples. Baishideng Publishing Group Inc 2021-11-20 /pmc/articles/PMC8613714/ /pubmed/34888182 http://dx.doi.org/10.5662/wjm.v11.i6.294 Text en ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved. https://creativecommons.org/licenses/by-nc/4.0/This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
spellingShingle | Retrospective Study Vilibic-Cavlek, Tatjana Barbic, Ljubo Stevanovic, Vladimir Savic, Vladimir Mrzljak, Anna Bogdanic, Maja Tabain, Irena Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections |
title | Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections |
title_full | Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections |
title_fullStr | Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections |
title_full_unstemmed | Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections |
title_short | Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections |
title_sort | comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections |
topic | Retrospective Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8613714/ https://www.ncbi.nlm.nih.gov/pubmed/34888182 http://dx.doi.org/10.5662/wjm.v11.i6.294 |
work_keys_str_mv | AT vilibiccavlektatjana comparisonofindirectimmunofluorescenceandwesternblotmethodinthediagnosisofhantavirusinfections AT barbicljubo comparisonofindirectimmunofluorescenceandwesternblotmethodinthediagnosisofhantavirusinfections AT stevanovicvladimir comparisonofindirectimmunofluorescenceandwesternblotmethodinthediagnosisofhantavirusinfections AT savicvladimir comparisonofindirectimmunofluorescenceandwesternblotmethodinthediagnosisofhantavirusinfections AT mrzljakanna comparisonofindirectimmunofluorescenceandwesternblotmethodinthediagnosisofhantavirusinfections AT bogdanicmaja comparisonofindirectimmunofluorescenceandwesternblotmethodinthediagnosisofhantavirusinfections AT tabainirena comparisonofindirectimmunofluorescenceandwesternblotmethodinthediagnosisofhantavirusinfections |