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Cellulosic ethanol production by consortia of Scheffersomyces stipitis and engineered Zymomonas mobilis

BACKGROUND: As one of the clean and sustainable energies, lignocellulosic ethanol has achieved much attention around the world. The production of lignocellulosic ethanol does not compete with people for food, while the consumption of ethanol could contribute to the carbon dioxide emission reduction....

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Autores principales: Sun, Lingling, Wu, Bo, Zhang, Zengqin, Yan, Jing, Liu, Panting, Song, Chao, Shabbir, Samina, Zhu, Qili, Yang, Shihui, Peng, Nan, He, Mingxiong, Tan, Furong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8613960/
https://www.ncbi.nlm.nih.gov/pubmed/34823583
http://dx.doi.org/10.1186/s13068-021-02069-8
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author Sun, Lingling
Wu, Bo
Zhang, Zengqin
Yan, Jing
Liu, Panting
Song, Chao
Shabbir, Samina
Zhu, Qili
Yang, Shihui
Peng, Nan
He, Mingxiong
Tan, Furong
author_facet Sun, Lingling
Wu, Bo
Zhang, Zengqin
Yan, Jing
Liu, Panting
Song, Chao
Shabbir, Samina
Zhu, Qili
Yang, Shihui
Peng, Nan
He, Mingxiong
Tan, Furong
author_sort Sun, Lingling
collection PubMed
description BACKGROUND: As one of the clean and sustainable energies, lignocellulosic ethanol has achieved much attention around the world. The production of lignocellulosic ethanol does not compete with people for food, while the consumption of ethanol could contribute to the carbon dioxide emission reduction. However, the simultaneous transformation of glucose and xylose to ethanol is one of the key technologies for attaining cost-efficient lignocellulosic ethanol production at an industrial scale. Genetic modification of strains and constructing consortia were two approaches to resolve this issue. Compared with strain improvement, the synergistic interaction of consortia in metabolic pathways should be more useful than using each one separately. RESULTS: In this study, the consortia consisting of suspended Scheffersomyces stipitis CICC1960 and Zymomonas mobilis 8b were cultivated to successfully depress carbon catabolite repression (CCR) in artificially simulated 80G40XRM. With this strategy, a 5.52% more xylose consumption and a 6.52% higher ethanol titer were achieved by the consortium, in which the inoculation ratio between S. stipitis and Z. mobilis was 1:3, compared with the Z. mobilis 8b mono-fermentation. Subsequently, one copy of the xylose metabolic genes was inserted into the Z. mobilis 8b genome to construct Z. mobilis FR2, leading to the xylose final-consumption amount and ethanol titer improvement by 15.36% and 6.81%, respectively. Finally, various corn stover hydrolysates with different sugar concentrations (glucose and xylose 60, 90, 120 g/L), were used to evaluate the fermentation performance of the consortium consisting of S. stipitis CICC1960 and Z. mobilis FR2. Fermentation results showed that a 1.56–4.59% higher ethanol titer was achieved by the consortium compared with the Z. mobilis FR2 mono-fermentation, and a 46.12–102.14% higher ethanol titer was observed in the consortium fermentation when compared with the S. stipitis CICC1960 mono-fermentation. Furthermore, qRT-PCR analysis of xylose/glucose transporter and other genes responsible for CCR explained the reason why the initial ratio inoculation of 1:3 in artificially simulated 80G40XRM had the best fermentation performance in the consortium. CONCLUSIONS: The fermentation strategy used in this study, i.e., using a genetically modified consortium, had a superior performance in ethanol production, as compared with the S. stipitis CICC1960 mono-fermentation and the Z. mobilis FR2 mono-fermentation alone. This result showed that this strategy has potential for future lignocellulosic ethanol production. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13068-021-02069-8.
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spelling pubmed-86139602021-11-29 Cellulosic ethanol production by consortia of Scheffersomyces stipitis and engineered Zymomonas mobilis Sun, Lingling Wu, Bo Zhang, Zengqin Yan, Jing Liu, Panting Song, Chao Shabbir, Samina Zhu, Qili Yang, Shihui Peng, Nan He, Mingxiong Tan, Furong Biotechnol Biofuels Research BACKGROUND: As one of the clean and sustainable energies, lignocellulosic ethanol has achieved much attention around the world. The production of lignocellulosic ethanol does not compete with people for food, while the consumption of ethanol could contribute to the carbon dioxide emission reduction. However, the simultaneous transformation of glucose and xylose to ethanol is one of the key technologies for attaining cost-efficient lignocellulosic ethanol production at an industrial scale. Genetic modification of strains and constructing consortia were two approaches to resolve this issue. Compared with strain improvement, the synergistic interaction of consortia in metabolic pathways should be more useful than using each one separately. RESULTS: In this study, the consortia consisting of suspended Scheffersomyces stipitis CICC1960 and Zymomonas mobilis 8b were cultivated to successfully depress carbon catabolite repression (CCR) in artificially simulated 80G40XRM. With this strategy, a 5.52% more xylose consumption and a 6.52% higher ethanol titer were achieved by the consortium, in which the inoculation ratio between S. stipitis and Z. mobilis was 1:3, compared with the Z. mobilis 8b mono-fermentation. Subsequently, one copy of the xylose metabolic genes was inserted into the Z. mobilis 8b genome to construct Z. mobilis FR2, leading to the xylose final-consumption amount and ethanol titer improvement by 15.36% and 6.81%, respectively. Finally, various corn stover hydrolysates with different sugar concentrations (glucose and xylose 60, 90, 120 g/L), were used to evaluate the fermentation performance of the consortium consisting of S. stipitis CICC1960 and Z. mobilis FR2. Fermentation results showed that a 1.56–4.59% higher ethanol titer was achieved by the consortium compared with the Z. mobilis FR2 mono-fermentation, and a 46.12–102.14% higher ethanol titer was observed in the consortium fermentation when compared with the S. stipitis CICC1960 mono-fermentation. Furthermore, qRT-PCR analysis of xylose/glucose transporter and other genes responsible for CCR explained the reason why the initial ratio inoculation of 1:3 in artificially simulated 80G40XRM had the best fermentation performance in the consortium. CONCLUSIONS: The fermentation strategy used in this study, i.e., using a genetically modified consortium, had a superior performance in ethanol production, as compared with the S. stipitis CICC1960 mono-fermentation and the Z. mobilis FR2 mono-fermentation alone. This result showed that this strategy has potential for future lignocellulosic ethanol production. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13068-021-02069-8. BioMed Central 2021-11-25 /pmc/articles/PMC8613960/ /pubmed/34823583 http://dx.doi.org/10.1186/s13068-021-02069-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Sun, Lingling
Wu, Bo
Zhang, Zengqin
Yan, Jing
Liu, Panting
Song, Chao
Shabbir, Samina
Zhu, Qili
Yang, Shihui
Peng, Nan
He, Mingxiong
Tan, Furong
Cellulosic ethanol production by consortia of Scheffersomyces stipitis and engineered Zymomonas mobilis
title Cellulosic ethanol production by consortia of Scheffersomyces stipitis and engineered Zymomonas mobilis
title_full Cellulosic ethanol production by consortia of Scheffersomyces stipitis and engineered Zymomonas mobilis
title_fullStr Cellulosic ethanol production by consortia of Scheffersomyces stipitis and engineered Zymomonas mobilis
title_full_unstemmed Cellulosic ethanol production by consortia of Scheffersomyces stipitis and engineered Zymomonas mobilis
title_short Cellulosic ethanol production by consortia of Scheffersomyces stipitis and engineered Zymomonas mobilis
title_sort cellulosic ethanol production by consortia of scheffersomyces stipitis and engineered zymomonas mobilis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8613960/
https://www.ncbi.nlm.nih.gov/pubmed/34823583
http://dx.doi.org/10.1186/s13068-021-02069-8
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