Corticosterone-Mediated Physiological Stress Alters Liver, Kidney, and Breast Muscle Metabolomic Profiles in Chickens

SIMPLE SUMMARY: Corticosterone is the major stress hormone in birds and research has shown that an increase in corticosterone can have adverse effects on bird health (e.g., predisposition to disease) production performance metrics. However, it is not currently possible to monitor commercial flocks for stress before performance is affected. A popular model of chicken stress involves administering corticosterone to chickens though their drinking water. However, corticosterone is non-polar so it must first be dissolved in ethanol, which means that the chickens are also drinking ethanol. In this study, an untargeted nuclear magnetic resonance-based metabolomics approach was used to investigate the effects of this model of stress in chickens, as well as the effects corticosterone on the chicken kidney, liver, and breast muscle metabolomes. We hypothesized that physiological stress modulates the metabolome of liver, kidney, and breast muscle due to increases in catabolism and gluconeogenesis. The administration of corticosterone altered the chicken liver, kidney, and breast muscle metabolomes. However, the ethanol carrier affected the metabolome of all three tissues, which indicated that corticosterone should be administered in an alternate fashion in future metabolomics studies to remove the confounding effects of ethanol. Furthermore, future research should focus on relating metabolite changes in tissues to non-destructive markers like blood, feces, or feathers to develop new diagnostic tools to better monitor on-farm stress during production. ABSTRACT: The impact of physiological stress on the metabolomes of liver, kidney, and breast muscle was investigated in chickens. To incite a stress response, birds were continuously administered corticosterone (CORT) in their drinking water at three doses (0, 10, and 30 mg L(−1)), and they were sampled 1, 5, and 12 days after the start of the CORT administration. To solubilize CORT, it was first dissolved in ethanol and then added to water. The administration of ethanol alone significantly altered branched chain amino acid metabolism in both the liver and the kidney, and amino acid and nitrogen metabolism in breast muscle. CORT significantly altered sugar and amino acid metabolism in all three tissues, but to a much greater degree than ethanol alone. In this regard, CORT administration significantly altered 11, 46, and 14 unique metabolites in liver, kidney, and breast muscle, respectively. Many of the metabolites that were affected by CORT administration, such as mannose and glucose, were previously linked to increases in glycosylation and gluconeogenesis in chickens under conditions of production stress. Moreover, several of these metabolites, such as dimethylglycine, galactose, and carnosine were also previously linked to reduced quality meat. In summary, the administration of CORT in chickens significantly modulated host metabolism. Moreover, results indicated that energy potentials are diverted from muscle anabolism to muscle catabolism and gluconeogenesis during periods of stress.