The Role of Birds of the Family Corvidae in Transmitting Sarcocystis Protozoan Parasites

SIMPLE SUMMARY: Members of the genus Sarcocystis are protozoan parasites that infect mammals, birds, and reptiles. Sarcocystis spp. have an obligatory two-host prey-predator life cycle. Sarcocysts form in the muscles and central nervous system of the intermediate host, while oocysts and sporocysts develop in the small intestine of the definitive host. There is a lack of studies on omnivorous birds of family Corvidae as potential definitive hosts of Sarcocystis spp. Until now, only S. ovalis has been confirmed to be transmitted via corvids. In the current study, 91 small intestine samples from six corvid species from Lithuania were examined for the presence of Sarcocystis spp. that use birds, carnivorous mammals, and cervids as intermediate hosts. Oocysts of Sarcocystis spp. were observed in 43 samples (47.3%) using a light microscope. Based on molecular methods, 11 Sarcocystis spp., (S. columbae, S. cornixi, S. halieti, S. kutkienae, S. lari, S. turdusi, S. wobeseri, S. arctica, S. lutrae, S. ovalis, and S. oviformis) were identified. These results indicate that corvids may transmit some species of Sarcocystis that use birds and mammals as intermediate hosts. ABSTRACT: Members of the family Corvidae are ecologically flexible omnivorous birds, particularly adaptive to urban habitats, and living in proximity to humans; these birds may serve as definitive hosts (DH) for Sarcocystis spp., but research about this is lacking. In the present study, intestinal samples from 91 corvids collected in Lithuania were molecularly tested by species-specific PCR targeting the ITS1 and cox1 genes and subsequently sequenced for the presence of Sarcocystis spp. Under a light microscope, oocysts of Sarcocystis spp. were observed in 43 samples (47.3%), while molecular methods, detected Sarcocystis spp. in 77 birds (84.6%). Eleven Sarcocystis spp. (S. columbae, S. cornixi, potentially pathogenic S. halieti, S. kutkienae, S. lari, S. turdusi, S. wobeseri, S. arctica, S. lutrae, S. ovalis, and S. oviformis) were identified in the intestinal samples from six corvid species from Lithuania. Infections with multiple Sarcocystis spp. were detected in 79.2% of the infected corvid birds. Three of the identified Sarcocystis spp. use corvids as intermediate hosts (IH); therefore, corvids may serve as IH and DH of the same Sarcocystis species. Based on molecular results and on corvid diet, omnivorous corvids may play an important role in transmitting Sarcocystis spp.