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Fermented Corn–Soybean Meal Mixed Feed Modulates Intestinal Morphology, Barrier Functions and Cecal Microbiota in Laying Hens

SIMPLE SUMMARY: Fermented feed has been of wide concern in livestock and poultry production because of its many advantages. In this study, the nutritional quality of the feed before and after fermentation was assessed, and four supplemental levels of fermented feed were used to replace unfermented f...

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Autores principales: Liu, Yinglu, Feng, Jia, Wang, Yamin, Lv, Jing, Li, Jinghe, Guo, Lijuan, Min, Yuna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614397/
https://www.ncbi.nlm.nih.gov/pubmed/34827791
http://dx.doi.org/10.3390/ani11113059
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author Liu, Yinglu
Feng, Jia
Wang, Yamin
Lv, Jing
Li, Jinghe
Guo, Lijuan
Min, Yuna
author_facet Liu, Yinglu
Feng, Jia
Wang, Yamin
Lv, Jing
Li, Jinghe
Guo, Lijuan
Min, Yuna
author_sort Liu, Yinglu
collection PubMed
description SIMPLE SUMMARY: Fermented feed has been of wide concern in livestock and poultry production because of its many advantages. In this study, the nutritional quality of the feed before and after fermentation was assessed, and four supplemental levels of fermented feed were used to replace unfermented feed to study the influence of fermented feed on the gut health of the laying hens during the laying peak period. The results suggest that fermented feed can improve the intestinal morphology and barrier functions of laying hens, possibly by altering the cecal microbiome. ABSTRACT: This study aimed to evaluate the effects of fermented corn–soybean meal mixed feed on intestinal barrier function and cecal microbiota in laying hens. A total of 360 Jingfen No.6 laying hens (22 wk-old) were assigned to 4 dietary treatments, which were offered basal diets (without antibiotics) containing 0, 4, 6 and 8% of fermented mixed feed respectively. The results showed that the pH value and anti-nutritional factor concentrations in fermented mixed feed were lower than those in unfermented feed (p < 0.05). Moreover, fermentation in the feed significantly increased the crude protein content (p < 0.05). Supplementation with fermented feed significantly reduced the crypt depth and increased the villi height:crypt depth ratio of duodenum and jejunum (p < 0.05). Meanwhile, fermented feed increased the secretory immunoglobulin A content and MUC2 mRNA expression of jejunum (p < 0.05). These beneficial effects were exhibited at the addition level ≥6% and microbial composition of caeca in the control, and so 6% fermented feed groups were analyzed. The structure of the gut microbiota was remarkably altered by additions, characterized by increased abundances of some health-promoting bacteria, such as Parasutterella, Butyricicoccus and Erysipelotrichaceae (p < 0.05). In summary, fermented mixed feed modulated cecal flora, subsequently contributing to improvements in intestinal morphology and barrier functions in laying hens.
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spelling pubmed-86143972021-11-26 Fermented Corn–Soybean Meal Mixed Feed Modulates Intestinal Morphology, Barrier Functions and Cecal Microbiota in Laying Hens Liu, Yinglu Feng, Jia Wang, Yamin Lv, Jing Li, Jinghe Guo, Lijuan Min, Yuna Animals (Basel) Article SIMPLE SUMMARY: Fermented feed has been of wide concern in livestock and poultry production because of its many advantages. In this study, the nutritional quality of the feed before and after fermentation was assessed, and four supplemental levels of fermented feed were used to replace unfermented feed to study the influence of fermented feed on the gut health of the laying hens during the laying peak period. The results suggest that fermented feed can improve the intestinal morphology and barrier functions of laying hens, possibly by altering the cecal microbiome. ABSTRACT: This study aimed to evaluate the effects of fermented corn–soybean meal mixed feed on intestinal barrier function and cecal microbiota in laying hens. A total of 360 Jingfen No.6 laying hens (22 wk-old) were assigned to 4 dietary treatments, which were offered basal diets (without antibiotics) containing 0, 4, 6 and 8% of fermented mixed feed respectively. The results showed that the pH value and anti-nutritional factor concentrations in fermented mixed feed were lower than those in unfermented feed (p < 0.05). Moreover, fermentation in the feed significantly increased the crude protein content (p < 0.05). Supplementation with fermented feed significantly reduced the crypt depth and increased the villi height:crypt depth ratio of duodenum and jejunum (p < 0.05). Meanwhile, fermented feed increased the secretory immunoglobulin A content and MUC2 mRNA expression of jejunum (p < 0.05). These beneficial effects were exhibited at the addition level ≥6% and microbial composition of caeca in the control, and so 6% fermented feed groups were analyzed. The structure of the gut microbiota was remarkably altered by additions, characterized by increased abundances of some health-promoting bacteria, such as Parasutterella, Butyricicoccus and Erysipelotrichaceae (p < 0.05). In summary, fermented mixed feed modulated cecal flora, subsequently contributing to improvements in intestinal morphology and barrier functions in laying hens. MDPI 2021-10-26 /pmc/articles/PMC8614397/ /pubmed/34827791 http://dx.doi.org/10.3390/ani11113059 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Yinglu
Feng, Jia
Wang, Yamin
Lv, Jing
Li, Jinghe
Guo, Lijuan
Min, Yuna
Fermented Corn–Soybean Meal Mixed Feed Modulates Intestinal Morphology, Barrier Functions and Cecal Microbiota in Laying Hens
title Fermented Corn–Soybean Meal Mixed Feed Modulates Intestinal Morphology, Barrier Functions and Cecal Microbiota in Laying Hens
title_full Fermented Corn–Soybean Meal Mixed Feed Modulates Intestinal Morphology, Barrier Functions and Cecal Microbiota in Laying Hens
title_fullStr Fermented Corn–Soybean Meal Mixed Feed Modulates Intestinal Morphology, Barrier Functions and Cecal Microbiota in Laying Hens
title_full_unstemmed Fermented Corn–Soybean Meal Mixed Feed Modulates Intestinal Morphology, Barrier Functions and Cecal Microbiota in Laying Hens
title_short Fermented Corn–Soybean Meal Mixed Feed Modulates Intestinal Morphology, Barrier Functions and Cecal Microbiota in Laying Hens
title_sort fermented corn–soybean meal mixed feed modulates intestinal morphology, barrier functions and cecal microbiota in laying hens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614397/
https://www.ncbi.nlm.nih.gov/pubmed/34827791
http://dx.doi.org/10.3390/ani11113059
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