Fibropapillomatosis and Chelonid Alphaherpesvirus 5 Infection in Kemp’s Ridley Sea Turtles (Lepidochelys kempii)

SIMPLE SUMMARY: The Kemp’s ridley sea turtle is an endangered species that is susceptible to a tumor disease called fibropapillomatosis (FP) and its associated virus, chelonid alphaherpesvirus 5 (ChHV5). The goal of our study was to describe FP in Kemp’s ridley turtles, including estimated disease prevalence and pathologyg, and case demographics and outcomes, to better understand the risk posed by FP to Kemp’s ridley population recovery. During 2006–2020, we identified 22 cases of Kemp’s ridley turtles with FP, including 12 adult turtles, a reproductively valuable age class. Molecular diagnostics were used to identify ChHV5 DNA in blood (7.8%) and tumor (91.7%) samples collected from free-ranging Kemp’s ridley turtles. Genomic sequencing was conducted to identify ChHV5 variants in tumor samples collected from Kemp’s ridley turtles with FP. Along with case data, phylogenetic analysis of resultant sequences suggests increasing, spatiotemporal spread of ChHV5 infections and FP among Kemp’s ridley turtles in coastal areas, including the Gulf of Mexico and the southwestern Atlantic Ocean, where they share habitat with green sea turtles (in which FP is enzootic). This is concerning because FP has an uncertain pathogenesis, is potentially related to anthropogenic environmental degradation, and can cause suffering and/or death in severely afflicted turtles. ABSTRACT: Fibropapillomatosis (FP), a debilitating, infectious neoplastic disease, is rarely reported in endangered Kemp’s ridley sea turtles (Lepidochelys kempii). With this study, we describe FP and the associated chelonid alphaherpesvirus 5 (ChHV5) in Kemp’s ridley turtles encountered in the United States during 2006–2020. Analysis of 22 case reports of Kemp’s ridley turtles with FP revealed that while the disease was mild in most cases, 54.5% were adult turtles, a reproductively valuable age class whose survival is a priority for population recovery. Of 51 blood samples from tumor-free turtles and 12 tumor samples from turtles with FP, 7.8% and 91.7%, respectively, tested positive for ChHV5 DNA via quantitative polymerase chain reaction (qPCR). Viral genome shotgun sequencing and phylogenetic analysis of six tumor samples show that ChHV5 sequences in Kemp’s ridley turtles encountered in the Gulf of Mexico and northwestern Atlantic cluster with ChHV5 sequences identified in green (Chelonia mydas) and loggerhead (Caretta caretta) sea turtles from Hawaii, the southwestern Atlantic Ocean, and the Caribbean. Results suggest an interspecific, spatiotemporal spread of FP among Kemp’s ridley turtles in regions where the disease is enzootic. Although FP is currently uncommon in this species, it remains a health concern due to its uncertain pathogenesis and potential relationship with habitat degradation.