Polyphenols from Blumea laciniata Extended the Lifespan and Enhanced Resistance to Stress in Caenorhabditis elegans via the Insulin Signaling Pathway

Blumea laciniata is widely used as a folk medicine in Asia, but relevant literature on it is rarely reported. We confirmed that polyphenol extract (containing chlorogenic acid, rutin, and luteolin-4-O-glucoside) from B. laciniata (EBL) showed strong antioxidant ability in vitro. Hence, in this work,...

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Autores principales: Chen, Tao, Luo, Siyuan, Wang, Xiaoju, Zhou, Yiling, Dai, Yali, Zhou, Lijun, Feng, Shiling, Yuan, Ming, Ding, Chunbang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614712/
https://www.ncbi.nlm.nih.gov/pubmed/34829615
http://dx.doi.org/10.3390/antiox10111744
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author Chen, Tao
Luo, Siyuan
Wang, Xiaoju
Zhou, Yiling
Dai, Yali
Zhou, Lijun
Feng, Shiling
Yuan, Ming
Ding, Chunbang
author_facet Chen, Tao
Luo, Siyuan
Wang, Xiaoju
Zhou, Yiling
Dai, Yali
Zhou, Lijun
Feng, Shiling
Yuan, Ming
Ding, Chunbang
author_sort Chen, Tao
collection PubMed
description Blumea laciniata is widely used as a folk medicine in Asia, but relevant literature on it is rarely reported. We confirmed that polyphenol extract (containing chlorogenic acid, rutin, and luteolin-4-O-glucoside) from B. laciniata (EBL) showed strong antioxidant ability in vitro. Hence, in this work, we applied Caenorhabditis elegans to further investigate the antioxidant and anti-ageing abilities of EBL in vivo. The results showed that EBL enhanced the survival of C. elegans under thermal stress by 12.62% and sharply reduced the reactive oxygen species level as well as the content of malonaldehyde. Moreover, EBL increased the activities of antioxidant enzymes such as catalase and superoxide dismutase. Additionally, EBL promoted DAF-16, a transcription factor, into the nucleus. Besides, EBL extended the lifespan of C. elegans by 17.39%, showing an anti-ageing effect. Different mutants indicated that the insulin/IGF-1 signaling pathway participated in the antioxidant and anti-ageing effect of EBL on C. elegans.
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spelling pubmed-86147122021-11-26 Polyphenols from Blumea laciniata Extended the Lifespan and Enhanced Resistance to Stress in Caenorhabditis elegans via the Insulin Signaling Pathway Chen, Tao Luo, Siyuan Wang, Xiaoju Zhou, Yiling Dai, Yali Zhou, Lijun Feng, Shiling Yuan, Ming Ding, Chunbang Antioxidants (Basel) Article Blumea laciniata is widely used as a folk medicine in Asia, but relevant literature on it is rarely reported. We confirmed that polyphenol extract (containing chlorogenic acid, rutin, and luteolin-4-O-glucoside) from B. laciniata (EBL) showed strong antioxidant ability in vitro. Hence, in this work, we applied Caenorhabditis elegans to further investigate the antioxidant and anti-ageing abilities of EBL in vivo. The results showed that EBL enhanced the survival of C. elegans under thermal stress by 12.62% and sharply reduced the reactive oxygen species level as well as the content of malonaldehyde. Moreover, EBL increased the activities of antioxidant enzymes such as catalase and superoxide dismutase. Additionally, EBL promoted DAF-16, a transcription factor, into the nucleus. Besides, EBL extended the lifespan of C. elegans by 17.39%, showing an anti-ageing effect. Different mutants indicated that the insulin/IGF-1 signaling pathway participated in the antioxidant and anti-ageing effect of EBL on C. elegans. MDPI 2021-10-30 /pmc/articles/PMC8614712/ /pubmed/34829615 http://dx.doi.org/10.3390/antiox10111744 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Tao
Luo, Siyuan
Wang, Xiaoju
Zhou, Yiling
Dai, Yali
Zhou, Lijun
Feng, Shiling
Yuan, Ming
Ding, Chunbang
Polyphenols from Blumea laciniata Extended the Lifespan and Enhanced Resistance to Stress in Caenorhabditis elegans via the Insulin Signaling Pathway
title Polyphenols from Blumea laciniata Extended the Lifespan and Enhanced Resistance to Stress in Caenorhabditis elegans via the Insulin Signaling Pathway
title_full Polyphenols from Blumea laciniata Extended the Lifespan and Enhanced Resistance to Stress in Caenorhabditis elegans via the Insulin Signaling Pathway
title_fullStr Polyphenols from Blumea laciniata Extended the Lifespan and Enhanced Resistance to Stress in Caenorhabditis elegans via the Insulin Signaling Pathway
title_full_unstemmed Polyphenols from Blumea laciniata Extended the Lifespan and Enhanced Resistance to Stress in Caenorhabditis elegans via the Insulin Signaling Pathway
title_short Polyphenols from Blumea laciniata Extended the Lifespan and Enhanced Resistance to Stress in Caenorhabditis elegans via the Insulin Signaling Pathway
title_sort polyphenols from blumea laciniata extended the lifespan and enhanced resistance to stress in caenorhabditis elegans via the insulin signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614712/
https://www.ncbi.nlm.nih.gov/pubmed/34829615
http://dx.doi.org/10.3390/antiox10111744
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