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Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots

Metalloid contamination, such as arsenic poisoning, poses a significant environmental problem, reducing plant productivity and putting human health at risk. Phytohormones are known to regulate arsenic stress; however, the function of strigolactones (SLs) in arsenic stress tolerance in rice is rarely...

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Autores principales: Mostofa, Mohammad Golam, Ha, Chien Van, Rahman, Md. Mezanur, Nguyen, Kien Huu, Keya, Sanjida Sultana, Watanabe, Yasuko, Itouga, Misao, Hashem, Abeer, Abd_Allah, Elsayed Fathi, Fujita, Masayuki, Tran, Lam-Son Phan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614715/
https://www.ncbi.nlm.nih.gov/pubmed/34829686
http://dx.doi.org/10.3390/antiox10111815
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author Mostofa, Mohammad Golam
Ha, Chien Van
Rahman, Md. Mezanur
Nguyen, Kien Huu
Keya, Sanjida Sultana
Watanabe, Yasuko
Itouga, Misao
Hashem, Abeer
Abd_Allah, Elsayed Fathi
Fujita, Masayuki
Tran, Lam-Son Phan
author_facet Mostofa, Mohammad Golam
Ha, Chien Van
Rahman, Md. Mezanur
Nguyen, Kien Huu
Keya, Sanjida Sultana
Watanabe, Yasuko
Itouga, Misao
Hashem, Abeer
Abd_Allah, Elsayed Fathi
Fujita, Masayuki
Tran, Lam-Son Phan
author_sort Mostofa, Mohammad Golam
collection PubMed
description Metalloid contamination, such as arsenic poisoning, poses a significant environmental problem, reducing plant productivity and putting human health at risk. Phytohormones are known to regulate arsenic stress; however, the function of strigolactones (SLs) in arsenic stress tolerance in rice is rarely investigated. Here, we investigated shoot responses of wild-type (WT) and SL-deficient d10 and d17 rice mutants under arsenate stress to elucidate SLs’ roles in rice adaptation to arsenic. Under arsenate stress, the d10 and d17 mutants displayed severe growth abnormalities, including phenotypic aberrations, chlorosis and biomass loss, relative to WT. Arsenate stress activated the SL-biosynthetic pathway by enhancing the expression of SL-biosynthetic genes D10 and D17 in WT shoots. No differences in arsenic levels between WT and SL-biosynthetic mutants were found from Inductively Coupled Plasma-Mass Spectrometry analysis, demonstrating that the greater growth defects of mutant plants did not result from accumulated arsenic in shoots. The d10 and d17 plants had higher levels of reactive oxygen species, water loss, electrolyte leakage and membrane damage but lower activities of superoxide dismutase, ascorbate peroxidase, glutathione peroxidase and glutathione S-transferase than did the WT, implying that arsenate caused substantial oxidative stress in the SL mutants. Furthermore, WT plants had higher glutathione (GSH) contents and transcript levels of OsGSH1, OsGSH2, OsPCS1 and OsABCC1 in their shoots, indicating an upregulation of GSH-assisted arsenic sequestration into vacuoles. We conclude that arsenate stress activated SL biosynthesis, which led to enhanced arsenate tolerance through the stimulation of cellular antioxidant defense systems and vacuolar sequestration of arsenic, suggesting a novel role for SLs in rice adaptation to arsenic stress. Our findings have significant implications in the development of arsenic-resistant rice varieties for safe and sustainable rice production in arsenic-polluted soils.
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spelling pubmed-86147152021-11-26 Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots Mostofa, Mohammad Golam Ha, Chien Van Rahman, Md. Mezanur Nguyen, Kien Huu Keya, Sanjida Sultana Watanabe, Yasuko Itouga, Misao Hashem, Abeer Abd_Allah, Elsayed Fathi Fujita, Masayuki Tran, Lam-Son Phan Antioxidants (Basel) Article Metalloid contamination, such as arsenic poisoning, poses a significant environmental problem, reducing plant productivity and putting human health at risk. Phytohormones are known to regulate arsenic stress; however, the function of strigolactones (SLs) in arsenic stress tolerance in rice is rarely investigated. Here, we investigated shoot responses of wild-type (WT) and SL-deficient d10 and d17 rice mutants under arsenate stress to elucidate SLs’ roles in rice adaptation to arsenic. Under arsenate stress, the d10 and d17 mutants displayed severe growth abnormalities, including phenotypic aberrations, chlorosis and biomass loss, relative to WT. Arsenate stress activated the SL-biosynthetic pathway by enhancing the expression of SL-biosynthetic genes D10 and D17 in WT shoots. No differences in arsenic levels between WT and SL-biosynthetic mutants were found from Inductively Coupled Plasma-Mass Spectrometry analysis, demonstrating that the greater growth defects of mutant plants did not result from accumulated arsenic in shoots. The d10 and d17 plants had higher levels of reactive oxygen species, water loss, electrolyte leakage and membrane damage but lower activities of superoxide dismutase, ascorbate peroxidase, glutathione peroxidase and glutathione S-transferase than did the WT, implying that arsenate caused substantial oxidative stress in the SL mutants. Furthermore, WT plants had higher glutathione (GSH) contents and transcript levels of OsGSH1, OsGSH2, OsPCS1 and OsABCC1 in their shoots, indicating an upregulation of GSH-assisted arsenic sequestration into vacuoles. We conclude that arsenate stress activated SL biosynthesis, which led to enhanced arsenate tolerance through the stimulation of cellular antioxidant defense systems and vacuolar sequestration of arsenic, suggesting a novel role for SLs in rice adaptation to arsenic stress. Our findings have significant implications in the development of arsenic-resistant rice varieties for safe and sustainable rice production in arsenic-polluted soils. MDPI 2021-11-15 /pmc/articles/PMC8614715/ /pubmed/34829686 http://dx.doi.org/10.3390/antiox10111815 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mostofa, Mohammad Golam
Ha, Chien Van
Rahman, Md. Mezanur
Nguyen, Kien Huu
Keya, Sanjida Sultana
Watanabe, Yasuko
Itouga, Misao
Hashem, Abeer
Abd_Allah, Elsayed Fathi
Fujita, Masayuki
Tran, Lam-Son Phan
Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots
title Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots
title_full Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots
title_fullStr Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots
title_full_unstemmed Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots
title_short Strigolactones Modulate Cellular Antioxidant Defense Mechanisms to Mitigate Arsenate Toxicity in Rice Shoots
title_sort strigolactones modulate cellular antioxidant defense mechanisms to mitigate arsenate toxicity in rice shoots
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614715/
https://www.ncbi.nlm.nih.gov/pubmed/34829686
http://dx.doi.org/10.3390/antiox10111815
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