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Application of Bacteriophages on Shiga Toxin-Producing Escherichia coli (STEC) Biofilm

Shiga toxin-producing Escherichia coli are pathogenic bacteria able to form biofilms both on abiotic surfaces and on food, thus increasing risks for food consumers. Moreover, biofilms are difficult to remove and more resistant to antimicrobial agents compared to planktonic cells. Bacteriophages, nat...

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Detalles Bibliográficos
Autores principales: Mangieri, Nicola, Foschino, Roberto, Picozzi, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614735/
https://www.ncbi.nlm.nih.gov/pubmed/34827361
http://dx.doi.org/10.3390/antibiotics10111423
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author Mangieri, Nicola
Foschino, Roberto
Picozzi, Claudia
author_facet Mangieri, Nicola
Foschino, Roberto
Picozzi, Claudia
author_sort Mangieri, Nicola
collection PubMed
description Shiga toxin-producing Escherichia coli are pathogenic bacteria able to form biofilms both on abiotic surfaces and on food, thus increasing risks for food consumers. Moreover, biofilms are difficult to remove and more resistant to antimicrobial agents compared to planktonic cells. Bacteriophages, natural predators of bacteria, can be used as an alternative to prevent biofilm formation or to remove pre-formed biofilm. In this work, four STEC able to produce biofilm were selected among 31 different strains and tested against single bacteriophages and two-phage cocktails. Results showed that our phages were able to reduce biofilm formation by 43.46% both when used as single phage preparation and as a cocktail formulation. Since one of the two cocktails had a slightly better performance, it was used to remove pre-existing biofilms. In this case, the phages were unable to destroy the biofilms and reduce the number of bacterial cells. Our data confirm that preventing biofilm formation in a food plant is better than trying to remove a preformed biofilm and the continuous presence of bacteriophages in the process environment could reduce the number of bacteria able to form biofilms and therefore improve the food safety.
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spelling pubmed-86147352021-11-26 Application of Bacteriophages on Shiga Toxin-Producing Escherichia coli (STEC) Biofilm Mangieri, Nicola Foschino, Roberto Picozzi, Claudia Antibiotics (Basel) Article Shiga toxin-producing Escherichia coli are pathogenic bacteria able to form biofilms both on abiotic surfaces and on food, thus increasing risks for food consumers. Moreover, biofilms are difficult to remove and more resistant to antimicrobial agents compared to planktonic cells. Bacteriophages, natural predators of bacteria, can be used as an alternative to prevent biofilm formation or to remove pre-formed biofilm. In this work, four STEC able to produce biofilm were selected among 31 different strains and tested against single bacteriophages and two-phage cocktails. Results showed that our phages were able to reduce biofilm formation by 43.46% both when used as single phage preparation and as a cocktail formulation. Since one of the two cocktails had a slightly better performance, it was used to remove pre-existing biofilms. In this case, the phages were unable to destroy the biofilms and reduce the number of bacterial cells. Our data confirm that preventing biofilm formation in a food plant is better than trying to remove a preformed biofilm and the continuous presence of bacteriophages in the process environment could reduce the number of bacteria able to form biofilms and therefore improve the food safety. MDPI 2021-11-20 /pmc/articles/PMC8614735/ /pubmed/34827361 http://dx.doi.org/10.3390/antibiotics10111423 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mangieri, Nicola
Foschino, Roberto
Picozzi, Claudia
Application of Bacteriophages on Shiga Toxin-Producing Escherichia coli (STEC) Biofilm
title Application of Bacteriophages on Shiga Toxin-Producing Escherichia coli (STEC) Biofilm
title_full Application of Bacteriophages on Shiga Toxin-Producing Escherichia coli (STEC) Biofilm
title_fullStr Application of Bacteriophages on Shiga Toxin-Producing Escherichia coli (STEC) Biofilm
title_full_unstemmed Application of Bacteriophages on Shiga Toxin-Producing Escherichia coli (STEC) Biofilm
title_short Application of Bacteriophages on Shiga Toxin-Producing Escherichia coli (STEC) Biofilm
title_sort application of bacteriophages on shiga toxin-producing escherichia coli (stec) biofilm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614735/
https://www.ncbi.nlm.nih.gov/pubmed/34827361
http://dx.doi.org/10.3390/antibiotics10111423
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