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High Genomic Identity between Clinical and Environmental Strains of Herbaspirillum frisingense Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs

The genus Herbaspirillum is widely studied for its ability to associate with grasses and to perform biological nitrogen fixation. However, the bacteria of the Herbaspirillum genus have frequently been isolated from clinical samples. Understanding the genomic characteristics that allow these bacteria...

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Autores principales: Oliveira, Willian Klassen, Ávila, Hugo Leonardo, Tadra, Michelle Zibeti, Cardoso, Rodrigo Luiz, Fadel-Pichet, Cyntia Maria Teles, de Souza, Emanuel Maltempi, de Oliveira Pedrosa, Fábio, Faoro, Helisson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614823/
https://www.ncbi.nlm.nih.gov/pubmed/34827347
http://dx.doi.org/10.3390/antibiotics10111409
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author Oliveira, Willian Klassen
Ávila, Hugo Leonardo
Tadra, Michelle Zibeti
Cardoso, Rodrigo Luiz
Fadel-Pichet, Cyntia Maria Teles
de Souza, Emanuel Maltempi
de Oliveira Pedrosa, Fábio
Faoro, Helisson
author_facet Oliveira, Willian Klassen
Ávila, Hugo Leonardo
Tadra, Michelle Zibeti
Cardoso, Rodrigo Luiz
Fadel-Pichet, Cyntia Maria Teles
de Souza, Emanuel Maltempi
de Oliveira Pedrosa, Fábio
Faoro, Helisson
author_sort Oliveira, Willian Klassen
collection PubMed
description The genus Herbaspirillum is widely studied for its ability to associate with grasses and to perform biological nitrogen fixation. However, the bacteria of the Herbaspirillum genus have frequently been isolated from clinical samples. Understanding the genomic characteristics that allow these bacteria to switch environments and become able to colonize human hosts is essential for monitoring emerging pathogens and predicting outbreaks. In this work, we describe the sequencing, assembly, and annotation of the genome of H. frisingense AU14559 isolated from the sputum of patients with cystic fibrosis, and its comparison with the genomes of the uropathogenic strain VT-16–41 and the environmental strains GSF30, BH-1, IAC152, and SG826. The genes responsible for biological nitrogen fixation were absent from all strains except for GSF30. On the other hand, genes encoding virulence and host interaction factors were mostly shared with environmental strains. We also identified a large set of intrinsic antibiotic resistance genes that were shared across all strains. Unlike other strains, in addition to unique genomic islands, AU14559 has a mutation that renders the biosynthesis of rhamnose and its incorporation into the exopolysaccharide unfeasible. These data suggest that H. frisingense has characteristics that provide it with the metabolic diversity needed to infect and colonize human hosts.
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spelling pubmed-86148232021-11-26 High Genomic Identity between Clinical and Environmental Strains of Herbaspirillum frisingense Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs Oliveira, Willian Klassen Ávila, Hugo Leonardo Tadra, Michelle Zibeti Cardoso, Rodrigo Luiz Fadel-Pichet, Cyntia Maria Teles de Souza, Emanuel Maltempi de Oliveira Pedrosa, Fábio Faoro, Helisson Antibiotics (Basel) Article The genus Herbaspirillum is widely studied for its ability to associate with grasses and to perform biological nitrogen fixation. However, the bacteria of the Herbaspirillum genus have frequently been isolated from clinical samples. Understanding the genomic characteristics that allow these bacteria to switch environments and become able to colonize human hosts is essential for monitoring emerging pathogens and predicting outbreaks. In this work, we describe the sequencing, assembly, and annotation of the genome of H. frisingense AU14559 isolated from the sputum of patients with cystic fibrosis, and its comparison with the genomes of the uropathogenic strain VT-16–41 and the environmental strains GSF30, BH-1, IAC152, and SG826. The genes responsible for biological nitrogen fixation were absent from all strains except for GSF30. On the other hand, genes encoding virulence and host interaction factors were mostly shared with environmental strains. We also identified a large set of intrinsic antibiotic resistance genes that were shared across all strains. Unlike other strains, in addition to unique genomic islands, AU14559 has a mutation that renders the biosynthesis of rhamnose and its incorporation into the exopolysaccharide unfeasible. These data suggest that H. frisingense has characteristics that provide it with the metabolic diversity needed to infect and colonize human hosts. MDPI 2021-11-18 /pmc/articles/PMC8614823/ /pubmed/34827347 http://dx.doi.org/10.3390/antibiotics10111409 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Oliveira, Willian Klassen
Ávila, Hugo Leonardo
Tadra, Michelle Zibeti
Cardoso, Rodrigo Luiz
Fadel-Pichet, Cyntia Maria Teles
de Souza, Emanuel Maltempi
de Oliveira Pedrosa, Fábio
Faoro, Helisson
High Genomic Identity between Clinical and Environmental Strains of Herbaspirillum frisingense Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs
title High Genomic Identity between Clinical and Environmental Strains of Herbaspirillum frisingense Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs
title_full High Genomic Identity between Clinical and Environmental Strains of Herbaspirillum frisingense Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs
title_fullStr High Genomic Identity between Clinical and Environmental Strains of Herbaspirillum frisingense Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs
title_full_unstemmed High Genomic Identity between Clinical and Environmental Strains of Herbaspirillum frisingense Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs
title_short High Genomic Identity between Clinical and Environmental Strains of Herbaspirillum frisingense Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs
title_sort high genomic identity between clinical and environmental strains of herbaspirillum frisingense suggests pre-adaptation to different hosts and intrinsic resistance to multiple drugs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614823/
https://www.ncbi.nlm.nih.gov/pubmed/34827347
http://dx.doi.org/10.3390/antibiotics10111409
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