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Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation

Silica dioxide nanoparticles (SiONPs) have been increasingly used in various industries; however, this has raised concerns regarding their potential toxicity. SiONPs are also a major component in the Asian sand dust that causes pulmonary diseases among the general public. Melatonin exerts some inhib...

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Autores principales: Lim, Je-Oh, Lee, Se-Jin, Kim, Woong-Il, Pak, So-Won, Kim, Jong-Choon, Kim, Joong-Sun, Cho, Young-Kwon, Lee, In-Chul, Shin, In-Sik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614841/
https://www.ncbi.nlm.nih.gov/pubmed/34829636
http://dx.doi.org/10.3390/antiox10111765
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author Lim, Je-Oh
Lee, Se-Jin
Kim, Woong-Il
Pak, So-Won
Kim, Jong-Choon
Kim, Joong-Sun
Cho, Young-Kwon
Lee, In-Chul
Shin, In-Sik
author_facet Lim, Je-Oh
Lee, Se-Jin
Kim, Woong-Il
Pak, So-Won
Kim, Jong-Choon
Kim, Joong-Sun
Cho, Young-Kwon
Lee, In-Chul
Shin, In-Sik
author_sort Lim, Je-Oh
collection PubMed
description Silica dioxide nanoparticles (SiONPs) have been increasingly used in various industries; however, this has raised concerns regarding their potential toxicity. SiONPs are also a major component in the Asian sand dust that causes pulmonary diseases among the general public. Melatonin exerts some inhibitory effects against lung inflammation. In this study, we explored the therapeutic properties of melatonin against lung inflammation using an SiONPs-induced lung inflammation murine model and SiONPs-stimulated H292 cells, human airway epithelial cell line, by focusing on the involvement of thioredoxin-interacting protein (TXNIP) in the modulation of the MAPKs/AP-1 axis. We induced an inflammatory response by exposing mouse lungs and the H292 cells to SiONPs and confirmed the anti-inflammatory effect of melatonin. Melatonin inhibited the expression of various inflammatory mediators, including TNF-α, IL-6, and IL-1β, in SiONPs-exposed mice and SiONPs-stimulated H292 cells; this inhibition contributed to a decline in inflammatory cell accumulation in the lung tissues. Furthermore, melatonin treatment decreased the expression of MAPKs and AP-1 by downregulating TXNIP, eventually decreasing the production of SiONPs-induced inflammatory mediators. Overall, these data suggest that melatonin reduces SiONPs-induced lung inflammation by downregulating the TXNIP/MAPKs/AP-1 signalling pathway, thereby supporting the use of melatonin as an effective approach to control SiONPs-induced lung inflammation.
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spelling pubmed-86148412021-11-26 Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation Lim, Je-Oh Lee, Se-Jin Kim, Woong-Il Pak, So-Won Kim, Jong-Choon Kim, Joong-Sun Cho, Young-Kwon Lee, In-Chul Shin, In-Sik Antioxidants (Basel) Article Silica dioxide nanoparticles (SiONPs) have been increasingly used in various industries; however, this has raised concerns regarding their potential toxicity. SiONPs are also a major component in the Asian sand dust that causes pulmonary diseases among the general public. Melatonin exerts some inhibitory effects against lung inflammation. In this study, we explored the therapeutic properties of melatonin against lung inflammation using an SiONPs-induced lung inflammation murine model and SiONPs-stimulated H292 cells, human airway epithelial cell line, by focusing on the involvement of thioredoxin-interacting protein (TXNIP) in the modulation of the MAPKs/AP-1 axis. We induced an inflammatory response by exposing mouse lungs and the H292 cells to SiONPs and confirmed the anti-inflammatory effect of melatonin. Melatonin inhibited the expression of various inflammatory mediators, including TNF-α, IL-6, and IL-1β, in SiONPs-exposed mice and SiONPs-stimulated H292 cells; this inhibition contributed to a decline in inflammatory cell accumulation in the lung tissues. Furthermore, melatonin treatment decreased the expression of MAPKs and AP-1 by downregulating TXNIP, eventually decreasing the production of SiONPs-induced inflammatory mediators. Overall, these data suggest that melatonin reduces SiONPs-induced lung inflammation by downregulating the TXNIP/MAPKs/AP-1 signalling pathway, thereby supporting the use of melatonin as an effective approach to control SiONPs-induced lung inflammation. MDPI 2021-11-04 /pmc/articles/PMC8614841/ /pubmed/34829636 http://dx.doi.org/10.3390/antiox10111765 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lim, Je-Oh
Lee, Se-Jin
Kim, Woong-Il
Pak, So-Won
Kim, Jong-Choon
Kim, Joong-Sun
Cho, Young-Kwon
Lee, In-Chul
Shin, In-Sik
Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation
title Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation
title_full Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation
title_fullStr Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation
title_full_unstemmed Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation
title_short Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation
title_sort melatonin alleviates silica nanoparticle-induced lung inflammation via thioredoxin-interacting protein downregulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614841/
https://www.ncbi.nlm.nih.gov/pubmed/34829636
http://dx.doi.org/10.3390/antiox10111765
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