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Bacillus-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens

In this article, the supernatant of the soil-borne pathogen Bacillus mn14 was used as the catalyst for the synthesis of AgNPs. The antibacterial and antifungal activity of Bs-AgNPs was evaluated, in which S. viridans and R. solani showed susceptibility at 70 µL and 100 µL concentrations. Enzyme prop...

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Autores principales: Kabeerdass, Nivedhitha, Al Otaibi, Ahmed, Rajendran, Manikandan, Manikandan, Ayyar, Kashmery, Heba A., Rahman, Mohammed M., Madhu, P., Khan, Anish, Asiri, Abdullah M., Mathanmohun, Maghimaa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614847/
https://www.ncbi.nlm.nih.gov/pubmed/34827271
http://dx.doi.org/10.3390/antibiotics10111334
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author Kabeerdass, Nivedhitha
Al Otaibi, Ahmed
Rajendran, Manikandan
Manikandan, Ayyar
Kashmery, Heba A.
Rahman, Mohammed M.
Madhu, P.
Khan, Anish
Asiri, Abdullah M.
Mathanmohun, Maghimaa
author_facet Kabeerdass, Nivedhitha
Al Otaibi, Ahmed
Rajendran, Manikandan
Manikandan, Ayyar
Kashmery, Heba A.
Rahman, Mohammed M.
Madhu, P.
Khan, Anish
Asiri, Abdullah M.
Mathanmohun, Maghimaa
author_sort Kabeerdass, Nivedhitha
collection PubMed
description In this article, the supernatant of the soil-borne pathogen Bacillus mn14 was used as the catalyst for the synthesis of AgNPs. The antibacterial and antifungal activity of Bs-AgNPs was evaluated, in which S. viridans and R. solani showed susceptibility at 70 µL and 100 µL concentrations. Enzyme properties of the isolates, according to minimal inhibitory action and a growth-enhancing hormone–indole acetic acid (IAA) study of the isolates, were expressed in TLC as a purple color with an Rf value of 0.7. UV/Vis spectroscopy revealed the presence of small-sized AgNPs, with a surface plasmon resonance (SPR) peak at 450 nm. The particle size analyzer identified the average diameter of the particles as 40.2 nm. The X-ray diffraction study confirmed the crystalline nature and face-centered cubic type of the silver nanoparticle. Scanning electron microscopy characterized the globular, small, round shape of the silver nanoparticle. AFM revealed the two-dimensional topology of the silver nanoparticle with a characteristic size ranging around 50 nm. Confocal microscopy showed the cell-wall disruption of S. viridans treated with Bs-AgNPs. High-content screening and compound microscopy revealed the destruction of mycelia of R. solani after exposure to Bs-AgNPs. Furthermore, the Bs-AgNPs cured sheath blight disease by reducing lesion length and enhancing root and shoot length in Oryza sativa seeds. This soil-borne pathogen Bacillus-mediated synthesis approach of AgNPs appears to be cost-efficient, ecofriendly, and farmer-friendly, representing an easy way of providing valuable nutritious edibles in the future.
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spelling pubmed-86148472021-11-26 Bacillus-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens Kabeerdass, Nivedhitha Al Otaibi, Ahmed Rajendran, Manikandan Manikandan, Ayyar Kashmery, Heba A. Rahman, Mohammed M. Madhu, P. Khan, Anish Asiri, Abdullah M. Mathanmohun, Maghimaa Antibiotics (Basel) Article In this article, the supernatant of the soil-borne pathogen Bacillus mn14 was used as the catalyst for the synthesis of AgNPs. The antibacterial and antifungal activity of Bs-AgNPs was evaluated, in which S. viridans and R. solani showed susceptibility at 70 µL and 100 µL concentrations. Enzyme properties of the isolates, according to minimal inhibitory action and a growth-enhancing hormone–indole acetic acid (IAA) study of the isolates, were expressed in TLC as a purple color with an Rf value of 0.7. UV/Vis spectroscopy revealed the presence of small-sized AgNPs, with a surface plasmon resonance (SPR) peak at 450 nm. The particle size analyzer identified the average diameter of the particles as 40.2 nm. The X-ray diffraction study confirmed the crystalline nature and face-centered cubic type of the silver nanoparticle. Scanning electron microscopy characterized the globular, small, round shape of the silver nanoparticle. AFM revealed the two-dimensional topology of the silver nanoparticle with a characteristic size ranging around 50 nm. Confocal microscopy showed the cell-wall disruption of S. viridans treated with Bs-AgNPs. High-content screening and compound microscopy revealed the destruction of mycelia of R. solani after exposure to Bs-AgNPs. Furthermore, the Bs-AgNPs cured sheath blight disease by reducing lesion length and enhancing root and shoot length in Oryza sativa seeds. This soil-borne pathogen Bacillus-mediated synthesis approach of AgNPs appears to be cost-efficient, ecofriendly, and farmer-friendly, representing an easy way of providing valuable nutritious edibles in the future. MDPI 2021-11-01 /pmc/articles/PMC8614847/ /pubmed/34827271 http://dx.doi.org/10.3390/antibiotics10111334 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kabeerdass, Nivedhitha
Al Otaibi, Ahmed
Rajendran, Manikandan
Manikandan, Ayyar
Kashmery, Heba A.
Rahman, Mohammed M.
Madhu, P.
Khan, Anish
Asiri, Abdullah M.
Mathanmohun, Maghimaa
Bacillus-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens
title Bacillus-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens
title_full Bacillus-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens
title_fullStr Bacillus-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens
title_full_unstemmed Bacillus-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens
title_short Bacillus-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens
title_sort bacillus-mediated silver nanoparticle synthesis and its antagonistic activity against bacterial and fungal pathogens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614847/
https://www.ncbi.nlm.nih.gov/pubmed/34827271
http://dx.doi.org/10.3390/antibiotics10111334
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