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Photofungizides Based on Curcumin and Derivates Thereof against Candida albicans and Aspergillus niger

Fungal infections in humans, contamination of food and structural damage to buildings by fungi are associated with high costs for the general public. In addition, the increase in antifungal resistance towards conventional treatment raises the demand for new fungicidal methods. Here, we present the a...

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Autores principales: Schamberger, Barbara, Plaetzer, Kristjan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614998/
https://www.ncbi.nlm.nih.gov/pubmed/34827253
http://dx.doi.org/10.3390/antibiotics10111315
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author Schamberger, Barbara
Plaetzer, Kristjan
author_facet Schamberger, Barbara
Plaetzer, Kristjan
author_sort Schamberger, Barbara
collection PubMed
description Fungal infections in humans, contamination of food and structural damage to buildings by fungi are associated with high costs for the general public. In addition, the increase in antifungal resistance towards conventional treatment raises the demand for new fungicidal methods. Here, we present the antifungal use of Photodynamic Inactivation (PDI) based on the natural photosensitizer curcumin and a water-soluble positively charged derivative thereof (SA-CUR 12a) against two different model organisms; Candida albicans grown in a liquid culture and photo treated with a 435 nm LED light followed by counting of the colony-forming units and photoinactivation of tissue-like hyphal spheres of Aspergillus niger (diameter ~5 mm) with subsequent monitoring of colony growth. Curcumin (50 µM, no incubation period, i.p.) supplemented with 10% or 0.5% DMSO as well as SA-CUR 12a (50 µM no i.p or 5 min i.p.) triggered a photoantifungal effect of >4 log units towards C. albicans. At 100 µM, SA-CUR 12a (0 min or 5 min i.p.) achieved a reduction of >6 log units. Colonies of A. niger shrunk significantly during PDI treatment. Photoinactivation with 50 µM or 100 µM curcumin (+0.5% DMSO) resulted in complete growth inhibition. PDI using 20, 50 or 100 µM SA-CUR 12a (with or without 10% DMSO) also showed a significant reduction in colony area compared to the control after 48 h, although less pronounced compared to curcumin. In summary, PDI using curcumin or SA-CUR 12a against C. albicans or A. niger is a promising alternative to currently used fungicides, with the advantage of being very unlikely to induce resistance.
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spelling pubmed-86149982021-11-26 Photofungizides Based on Curcumin and Derivates Thereof against Candida albicans and Aspergillus niger Schamberger, Barbara Plaetzer, Kristjan Antibiotics (Basel) Article Fungal infections in humans, contamination of food and structural damage to buildings by fungi are associated with high costs for the general public. In addition, the increase in antifungal resistance towards conventional treatment raises the demand for new fungicidal methods. Here, we present the antifungal use of Photodynamic Inactivation (PDI) based on the natural photosensitizer curcumin and a water-soluble positively charged derivative thereof (SA-CUR 12a) against two different model organisms; Candida albicans grown in a liquid culture and photo treated with a 435 nm LED light followed by counting of the colony-forming units and photoinactivation of tissue-like hyphal spheres of Aspergillus niger (diameter ~5 mm) with subsequent monitoring of colony growth. Curcumin (50 µM, no incubation period, i.p.) supplemented with 10% or 0.5% DMSO as well as SA-CUR 12a (50 µM no i.p or 5 min i.p.) triggered a photoantifungal effect of >4 log units towards C. albicans. At 100 µM, SA-CUR 12a (0 min or 5 min i.p.) achieved a reduction of >6 log units. Colonies of A. niger shrunk significantly during PDI treatment. Photoinactivation with 50 µM or 100 µM curcumin (+0.5% DMSO) resulted in complete growth inhibition. PDI using 20, 50 or 100 µM SA-CUR 12a (with or without 10% DMSO) also showed a significant reduction in colony area compared to the control after 48 h, although less pronounced compared to curcumin. In summary, PDI using curcumin or SA-CUR 12a against C. albicans or A. niger is a promising alternative to currently used fungicides, with the advantage of being very unlikely to induce resistance. MDPI 2021-10-28 /pmc/articles/PMC8614998/ /pubmed/34827253 http://dx.doi.org/10.3390/antibiotics10111315 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schamberger, Barbara
Plaetzer, Kristjan
Photofungizides Based on Curcumin and Derivates Thereof against Candida albicans and Aspergillus niger
title Photofungizides Based on Curcumin and Derivates Thereof against Candida albicans and Aspergillus niger
title_full Photofungizides Based on Curcumin and Derivates Thereof against Candida albicans and Aspergillus niger
title_fullStr Photofungizides Based on Curcumin and Derivates Thereof against Candida albicans and Aspergillus niger
title_full_unstemmed Photofungizides Based on Curcumin and Derivates Thereof against Candida albicans and Aspergillus niger
title_short Photofungizides Based on Curcumin and Derivates Thereof against Candida albicans and Aspergillus niger
title_sort photofungizides based on curcumin and derivates thereof against candida albicans and aspergillus niger
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614998/
https://www.ncbi.nlm.nih.gov/pubmed/34827253
http://dx.doi.org/10.3390/antibiotics10111315
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