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Genetically Encoded Fluorescent Biosensors for Biomedical Applications

One of the challenges of modern biology and medicine is to visualize biomolecules in their natural environment, in real-time and in a non-invasive fashion, so as to gain insight into their physiological behavior and highlight alterations in pathological settings, which will enable to devise appropri...

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Autores principales: Ovechkina, Vera S., Zakian, Suren M., Medvedev, Sergey P., Valetdinova, Kamila R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615007/
https://www.ncbi.nlm.nih.gov/pubmed/34829757
http://dx.doi.org/10.3390/biomedicines9111528
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author Ovechkina, Vera S.
Zakian, Suren M.
Medvedev, Sergey P.
Valetdinova, Kamila R.
author_facet Ovechkina, Vera S.
Zakian, Suren M.
Medvedev, Sergey P.
Valetdinova, Kamila R.
author_sort Ovechkina, Vera S.
collection PubMed
description One of the challenges of modern biology and medicine is to visualize biomolecules in their natural environment, in real-time and in a non-invasive fashion, so as to gain insight into their physiological behavior and highlight alterations in pathological settings, which will enable to devise appropriate therapeutic strategies. Genetically encoded fluorescent biosensors constitute a class of imaging agents that enable visualization of biological processes and events directly in situ, preserving the native biological context and providing detailed insight into their localization and dynamics in cells. Real-time monitoring of drug action in a specific cellular compartment, organ, or tissue type; the ability to screen at the single-cell resolution; and the elimination of false-positive results caused by low drug bioavailability that is not detected by in vitro testing methods are a few of the obvious benefits of using genetically encoded fluorescent biosensors in drug screening. This review summarizes results of the studies that have been conducted in the last years toward the fabrication of genetically encoded fluorescent biosensors for biomedical applications with a comprehensive discussion on the challenges, future trends, and potential inputs needed for improving them.
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spelling pubmed-86150072021-11-26 Genetically Encoded Fluorescent Biosensors for Biomedical Applications Ovechkina, Vera S. Zakian, Suren M. Medvedev, Sergey P. Valetdinova, Kamila R. Biomedicines Review One of the challenges of modern biology and medicine is to visualize biomolecules in their natural environment, in real-time and in a non-invasive fashion, so as to gain insight into their physiological behavior and highlight alterations in pathological settings, which will enable to devise appropriate therapeutic strategies. Genetically encoded fluorescent biosensors constitute a class of imaging agents that enable visualization of biological processes and events directly in situ, preserving the native biological context and providing detailed insight into their localization and dynamics in cells. Real-time monitoring of drug action in a specific cellular compartment, organ, or tissue type; the ability to screen at the single-cell resolution; and the elimination of false-positive results caused by low drug bioavailability that is not detected by in vitro testing methods are a few of the obvious benefits of using genetically encoded fluorescent biosensors in drug screening. This review summarizes results of the studies that have been conducted in the last years toward the fabrication of genetically encoded fluorescent biosensors for biomedical applications with a comprehensive discussion on the challenges, future trends, and potential inputs needed for improving them. MDPI 2021-10-24 /pmc/articles/PMC8615007/ /pubmed/34829757 http://dx.doi.org/10.3390/biomedicines9111528 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Ovechkina, Vera S.
Zakian, Suren M.
Medvedev, Sergey P.
Valetdinova, Kamila R.
Genetically Encoded Fluorescent Biosensors for Biomedical Applications
title Genetically Encoded Fluorescent Biosensors for Biomedical Applications
title_full Genetically Encoded Fluorescent Biosensors for Biomedical Applications
title_fullStr Genetically Encoded Fluorescent Biosensors for Biomedical Applications
title_full_unstemmed Genetically Encoded Fluorescent Biosensors for Biomedical Applications
title_short Genetically Encoded Fluorescent Biosensors for Biomedical Applications
title_sort genetically encoded fluorescent biosensors for biomedical applications
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615007/
https://www.ncbi.nlm.nih.gov/pubmed/34829757
http://dx.doi.org/10.3390/biomedicines9111528
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