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Effect of Imipenem and Amikacin Combination against Multi-Drug Resistant Pseudomonas aeruginosa
Pseudomonas aeruginosa is an opportunistic nosocomial pathogen associated with high morbidity and mortality rates. Combination of antibiotics has been found to combat multi-drug resistant or extensively drug resistance P. aeruginosa. In this study we investigate the in vitro and in vivo effect of am...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615098/ https://www.ncbi.nlm.nih.gov/pubmed/34827367 http://dx.doi.org/10.3390/antibiotics10111429 |
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author | Farhan, Sara Mahmoud Raafat, Mohamed Abourehab, Mohammed A. S. Abd El-Baky, Rehab Mahmoud Abdalla, Salah EL-Gendy, Ahmed Osama Azmy, Ahmed Farag |
author_facet | Farhan, Sara Mahmoud Raafat, Mohamed Abourehab, Mohammed A. S. Abd El-Baky, Rehab Mahmoud Abdalla, Salah EL-Gendy, Ahmed Osama Azmy, Ahmed Farag |
author_sort | Farhan, Sara Mahmoud |
collection | PubMed |
description | Pseudomonas aeruginosa is an opportunistic nosocomial pathogen associated with high morbidity and mortality rates. Combination of antibiotics has been found to combat multi-drug resistant or extensively drug resistance P. aeruginosa. In this study we investigate the in vitro and in vivo effect of amikacin and imipenem combination against resistant P. aeruginosa. The checkerboard technique and time-killing curve have been performed for in vitro studies showed synergistic effect for combination. A peritonitis mouse model has been used for evaluation of the therapeutic efficacy of this combination which confirmed this synergistic effect. The in vitro and in vivo techniques showed synergistic interaction between tested drugs with fractional inhibitory concentration indices (FICIs) of ≤0.5. Conventional PCR and quantitative real-time PCR techniques were used in molecular detection of bla (IMP) and aac(6′)-Ib as 35.5% and 42.2% of P. aeruginosa harbored bla (IMP) and aac(6′)-Ib respectively. Drug combination viewed statistically significant reduction in bacterial counts (p value < 0.5). The lowest bla (IMP) and aac(6′)-Ib expression was observed after treatment with 0.25 MIC of imipenem + 0.5 MIC of amikacin. Morphological changes in P. aeruginosa isolates were detected by scanning electron microscope (SEM) showing cell shrinkage and disruption in the outer membrane of P. aeruginosa that were more prominent with combination therapy than with monotherapy. |
format | Online Article Text |
id | pubmed-8615098 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86150982021-11-26 Effect of Imipenem and Amikacin Combination against Multi-Drug Resistant Pseudomonas aeruginosa Farhan, Sara Mahmoud Raafat, Mohamed Abourehab, Mohammed A. S. Abd El-Baky, Rehab Mahmoud Abdalla, Salah EL-Gendy, Ahmed Osama Azmy, Ahmed Farag Antibiotics (Basel) Article Pseudomonas aeruginosa is an opportunistic nosocomial pathogen associated with high morbidity and mortality rates. Combination of antibiotics has been found to combat multi-drug resistant or extensively drug resistance P. aeruginosa. In this study we investigate the in vitro and in vivo effect of amikacin and imipenem combination against resistant P. aeruginosa. The checkerboard technique and time-killing curve have been performed for in vitro studies showed synergistic effect for combination. A peritonitis mouse model has been used for evaluation of the therapeutic efficacy of this combination which confirmed this synergistic effect. The in vitro and in vivo techniques showed synergistic interaction between tested drugs with fractional inhibitory concentration indices (FICIs) of ≤0.5. Conventional PCR and quantitative real-time PCR techniques were used in molecular detection of bla (IMP) and aac(6′)-Ib as 35.5% and 42.2% of P. aeruginosa harbored bla (IMP) and aac(6′)-Ib respectively. Drug combination viewed statistically significant reduction in bacterial counts (p value < 0.5). The lowest bla (IMP) and aac(6′)-Ib expression was observed after treatment with 0.25 MIC of imipenem + 0.5 MIC of amikacin. Morphological changes in P. aeruginosa isolates were detected by scanning electron microscope (SEM) showing cell shrinkage and disruption in the outer membrane of P. aeruginosa that were more prominent with combination therapy than with monotherapy. MDPI 2021-11-22 /pmc/articles/PMC8615098/ /pubmed/34827367 http://dx.doi.org/10.3390/antibiotics10111429 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Farhan, Sara Mahmoud Raafat, Mohamed Abourehab, Mohammed A. S. Abd El-Baky, Rehab Mahmoud Abdalla, Salah EL-Gendy, Ahmed Osama Azmy, Ahmed Farag Effect of Imipenem and Amikacin Combination against Multi-Drug Resistant Pseudomonas aeruginosa |
title | Effect of Imipenem and Amikacin Combination against Multi-Drug Resistant Pseudomonas aeruginosa |
title_full | Effect of Imipenem and Amikacin Combination against Multi-Drug Resistant Pseudomonas aeruginosa |
title_fullStr | Effect of Imipenem and Amikacin Combination against Multi-Drug Resistant Pseudomonas aeruginosa |
title_full_unstemmed | Effect of Imipenem and Amikacin Combination against Multi-Drug Resistant Pseudomonas aeruginosa |
title_short | Effect of Imipenem and Amikacin Combination against Multi-Drug Resistant Pseudomonas aeruginosa |
title_sort | effect of imipenem and amikacin combination against multi-drug resistant pseudomonas aeruginosa |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615098/ https://www.ncbi.nlm.nih.gov/pubmed/34827367 http://dx.doi.org/10.3390/antibiotics10111429 |
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